Phytotherapy for Attention Deficit Hyperactivity Disorder (ADHD): A Systematic Review and Meta-analysis

Attention deficit hyperactivity disorder (ADHD) is commonly a neurodevelopmental behavioural disorder in children and adolescents. Mainly characterized by symptoms like lack of attention, hyperactivity, and impulsiveness, it can impact the overall mental development of the one affected. Several factors, both genetic and non-genetic, can be responsible for this disorder. Although several traditional treatment methods involve medication and other counselling techniques, they also come with different side effects. Hence, the choice is now shifting to alternative treatment techniques. Herbal treatments are considered one of the most popular complementary and alternative medicine (CAM) administered. However, issues related to the safety and efficacy of herbal remedies for the treatment of ADHD need to be investigated further. This study aims to find out the recent advancement in evidence-based use of herbal remedies for ADHD by a comprehensive and systematic review that depicts the results of the published works on herbal therapy for the disorder. The electronic databases and the references retrieved from the included studies present related randomized controlled trials (RCTs) and open-label studies. Seven RCTs involving children and adolescents diagnosed with ADHD met the inclusion criteria. There is a fair indication of the efficacy and safety of Melissa officinalis L., Bacopa monnieri (L.) Wettst., Matricaria chamomilla L., and Valeriana officinalis L. from the studies evaluated in this systematic review for the treatment of various symptoms of ADHD. Limited evidence was found for Ginkgo biloba L. and pine bark extract. However, various other preparations from other plants did not show significant efficacy. There is inadequate proof to strongly support and recommend the administration of herbal medicines for ADHD, but more research is needed in the relevant field to popularize the alternative treatment approach

Genome editing technologies, mechanisms and improved production of therapeutic phytochemicals: Opportunities and prospects

Plants produce a large number of secondary metabolites, known as phytometabolites that may be employed as medicines, dyes, poisons, and insecticides in the field of medicine, agriculture, and industrial use, respectively. The rise of genome management approaches has promised a factual revolution in genetic engineering. Targeted genome editing in living entities permits the understanding of the biological systems very clearly, and also sanctions to address a wide-ranging objective in the direction of improving features of plant and their yields. The last few years have introduced a number of unique genome editing systems, including transcription activator-like effector nucleases, zinc finger nucleases, and miRNA-regulated clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). Genome editing systems have helped in the transformation of metabolic engineering, allowing researchers to modify biosynthetic pathways of different secondary metabolites. Given the growing relevance of editing genomes in plant research, the exciting novel methods are briefly reviewed in this chapter. Also, this chapter highlights recent discoveries on the CRISPR-based modification of natural products in different medicinal plants.All the authors are highly grateful and acknowledge the authority ofthe respective departments and institutions for their support incarrying out this study. This research was funded by projectsAPOGEO (Cooperation Program INTERREG‐MAC 2014–2020, withEuropean Funds for Regional Development‐FEDER).“Agencia Canaria de Investigación, Innova‐ción y Sociedad de la Información (ACIISI)del Gobierno de Canarias”(Project ProID2020010134), and Fundación CajaCanarias (Project 2019SP43).Peer reviewe

Traditional uses, phytochemistry, pharmacology and toxicology of garlic (Allium sativum), a storehouse of diverse phytochemicals: A review of research from the last decade focusing on health and nutritional implications

Allium sativum L. (Garlic) is a fragrant herb and tuber-derived spice that is one of the most sought-after botanicals, used as a culinary and ethnomedicine for a variety of diseases around the world. An array of pharmacological attributes such as antioxidant, hypoglycemic, anti-inflammatory, antihyperlipidemic, anticancer, antimicrobial, and hepatoprotective activities of this species have been established by previous studies. A. sativum houses many sulfur-containing phytochemical compounds such as allicin, diallyl disulfide (DADS), vinyldithiins, ajoenes (E-ajoene, Z-ajoene), diallyl trisulfide (DATS), micronutrient selenium (Se) etc. Organosulfur compounds are correlated with modulations in its antioxidant properties. The garlic compounds have also been recorded as promising immune-boosters or act as potent immunostimulants. A. sativum helps to treat cardiovascular ailments, neoplastic growth, rheumatism, diabetes, intestinal worms, flatulence, colic, dysentery, liver diseases, facial paralysis, tuberculosis, bronchitis, high blood pressure, and several other diseases. The present review aims to comprehensively enumerate the ethnobotanical and pharmacological aspects of A. sativum with notes on its phytochemistry, ethnopharmacology, toxicological aspects, and clinical studies from the retrieved literature from the last decade with notes on recent breakthroughs and bottlenecks. Future directions related to garlic research is also discussed.This work was supported by the UHK (Project No. VT2019- 2021), APOGEO (Cooperation Program INTERREG-MAC 2014–2020), with European Funds for Regional Development- FEDER, the “Agencia Canaria de Investigación, Innovación y Sociedad de la Información (ACIISI) Gobierno de Canarias” (Project No. ProID2020010134), and Caja Canarias (Project No. 2019SP43).Peer reviewe

Cytokinin and abiotic stress tolerance -What has been accomplished and the way forward?

More than a half-century has passed since it was discovered that phytohormone cytokinin (CK) is essential to drive cytokinesis and proliferation in plant tissue culture. Thereafter, cytokinin has emerged as the primary regulator of the plant cell cycle and numerous developmental processes. Lately, a growing body of evidence suggests that cytokinin has a role in mitigating both abiotic and biotic stress. Cytokinin is essential to defend plants against excessive light exposure and a unique kind of abiotic stress generated by an altered photoperiod. Secondly, cytokinin also exhibits multi-stress resilience under changing environments. Furthermore, cytokinin homeostasis is also affected by several forms of stress. Therefore, the diverse roles of cytokinin in reaction to stress, as well as its interactions with other hormones, are discussed in detail. When it comes to agriculture, understanding the functioning processes of cytokinins under changing environmental conditions can assist in utilizing the phytohormone, to increase productivity. Through this review, we briefly describe the biological role of cytokinin in enhancing the performance of plants growth under abiotic challenges as well as the probable mechanisms underpinning cytokinin-induced stress tolerance. In addition, the article lays forth a strategy for using biotechnological tools to modify genes in the cytokinin pathway to engineer abiotic stress tolerance in plants. The information presented here will assist in better understanding the function of cytokinin in plants and their effective investigation in the cropping system

Multiple roles for basement membrane proteins in cancer progression and EMT

Metastasis or the progression of malignancy poses a major challenge in cancer therapy and is the principal reason for increased mortality. The epithelial-mesenchymal transition (EMT) of the basement membrane (BM) allows cells of epithelial phenotype to transform into a mesenchymal-like (quasi-mesenchymal) phenotype and metastasize via the lymphovascular system through a metastatic cascade by intravasation and extravasation. This helps in the progression of carcinoma from the primary site to distant organs. Collagen, laminin, and integrin are the prime components of BM and help in tumor cell metastasis, which makes them ideal cancer drug targets. Further, recent studies have shown that collagen, laminin, and integrin can be used as a biomarker for metastatic cells. In this review, we have summarized the current knowledge of such therapeutics, which are either currently in preclinical or clinical stages and could be promising cancer therapeutics.Data availability: Not applicableScientific Research at Majmaah University [R-2022-117]Dr. Niraj Kumar Jha is thankful to Sharda University for the infra-structure and facility. The author would like to thank Deanship of Sci-entific Research at Majmaah University for supporting this work under project number No. R-2022-117. The authors would like to acknowledge the support from their respective institutes throughout the review writing process

Beneficial Role of Selenium (Se) Biofortification in Developing Resilience Against Potentially Toxic Metal and Metalloid Stress in Crops: Recent Trends in Genetic Engineering and Omics Approaches

Selenium (Se) is found in plants both in inorganic and organic forms as selenoamino acids and methylated complexes. Se influences growth and physiological parameters, viz. root and shoot growth, starch accumulation, water status regulation, respiration, germination promotion, nitrogen assimilation and delaying senescence in plants. Se deficiency causes many human medical conditions, viz. cancer, liver disease, cardiovascular disease, thyroid disease, Keshan disease and central nervous system disorders. Plants need Se to alleviate abiotic stresses, viz. high/low temperatures, drought, salinity, light, UV-B radiation and toxic metals/metalloids. Ameliorative role of Se, applied in relatively low doses, against abiotic stresses was mediated via enzymatic and non-enzymatic antioxidation. Chemical form, application method, edaphic presence and bioavailability of Se and its interaction with other elements regulate Se biofortification and abiotic stress tolerance in food and medicinal crops. Popular scientific search engines, viz. PubMed, Pubget, ScienceDirect, Medline, Scopus, SpringerLink, Mendeley, EMBASE, Google Scholar and JSTOR were searched to retrieve published articles by using pre-determined search strings involving selenium, plants, stress, metals, metalloids, etc. The retrieved literature encompasses the role of Se biofortification in enhancing dietary Se in crops and its bioavailability. The review also presents recent trends in Se-mediated amelioration of toxic metal and metalloid stresses via maintaining cell membrane integrity, functioning of photosynthetic machinery, inhibition against uptake and translocation of toxic metals and metalloids, control over the uptake and distribution of mineral nutrition and conversion of toxic metals and metalloids to non-toxic Se-metal complexes. Furthermore, current research on the genetic engineering and omics studies on Se biofortification and Se-mediated abiotic stress tolerance are also discussed. The present review comprehensively elucidates the beneficial role Se biofortification in developing resilience in crops against toxic metals and metalloids.Presidency University, IndiaThis work was supported by the Faculty Research and Professional Development Fund (FRPDF), a financial assistance from Presidency University, India

Therapeutic properties and pharmacological activities of asiaticoside and madecassoside: A review

Centella asiatica is an ethnomedicinal herbaceous species that grows abundantly in tropical and sub-tropical regions of China, India, South-Eastern Asia and Africa. It is a popular nutraceutical that is employed in various forms of clinical and cosmetic treatments. C. asiatica extracts are reported widely in Ayurvedic and Chinese traditional medicine to boost memory, prevent cognitive deficits and improve brain functions. The major bioactive constituents of C. asiatica are the pentacyclic triterpenoid glycosides, asiaticoside and madecassoside, and their corresponding aglycones, asiatic acid and madecassic acid. Asiaticoside and madecassoside have been identified as the marker compounds of C. asiatica in the Chinese Pharmacopoeia and these triterpene compounds offer a wide range of pharmacological properties, including neuroprotective, cardioprotective, hepatoprotective, wound healing, anti-inflammatory, anti-oxidant, anti-allergic, anti-depressant, anxiolytic, antifibrotic, antibacterial, anti-arthritic, anti-tumour and immunomodulatory activities. Asiaticoside and madecassoside are also used extensively in treating skin abnormalities, burn injuries, ischaemia, ulcers, asthma, lupus, psoriasis and scleroderma. Besides medicinal applications, these phytocompounds are considered cosmetically beneficial for their role in anti-ageing, skin hydration, collagen synthesis, UV protection and curing scars. Existing reports and experimental studies on these compounds between 2005 and 2022 have been selectively reviewed in this article to provide a comprehensive overview of the numerous therapeutic advantages of asiaticoside and madecassoside and their potential roles in the medical future.This research was funded by project APOGEO (Cooperation Program INTERREG-MAC 2014-2020, with European Funds for Regional Development-FEDER, “Agencia Canaria de Investigación, Innovación y Sociedad de la Información (ACIISI) del Gobierno de Canarias”, project ProID2020010134, CajaCanarias, Project 2019SP43, and the State Plan for Scientific, Technical Research and Innovation 2021-2023 from the Spanish Ministry of Science and Innovation (project PLEC2022-009507).Peer reviewe