Merkel cell polyomavirus infection in a patient with Merkel cell carcinoma: A case report

Introduction: Merkel cell carcinoma (MCC) is a rare and highly aggressive malignancy of the skin which occurs mainly in old people and is very uncommon in young individuals. A new tumor virus belonging to the Polyomaviridae family; Merkel Cell Polyomavirus (MCPyV) has recently been identified in more than 80 of MCCs. Case Presentation: We conducted a retrospective review on the archives of the Department of Pathology; Imam Khomeini Hospital Cancer Institute affiliated to Tehran University of Medical Sciences to confirm the MCC samples and we found medical records and samples of a young case with MCC who developed leg skin and scalp tumor six and seven years after bone marrow transplantation, respectively. We analyzed patient formalin-fixed paraffin-embedded samples for the presence of MCPyV DNA using polymerase chain reaction (PCR) method, and the PCR amplicons were subjected to DNA sequencing. Merkel Cell Polyomavirus DNA was detected in both tumors from patient and sequence analysis of the viral LT3 region showed a close homology to strains circulating worldwide. Conclusions: The findings of this study are consistent with the hypothesis that local, systemic, or tumor-induced immunosuppression may allow the MCPyV to initiate skin aggressive cancer. It is necessary to maintain regular check over patients taking immunosuppressive medications for MCPyV infection. Since there is not any information about detection and molecular biology analysis of MCPyV among Iranian patients with MCC, this study provides more information about MCC and MCPyV in Iran. © 2015, Ahvaz Jundishapur University of Medical Sciences

A comparative study of long interspersed element-1 protein immunoreactivity in cutaneous malignancies

Background: Skin cancer is the most common cancer worldwide and commonly classified into malignant melanoma (MM) and Nonmelanoma skin cancers (NMSCs), which mainly include basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). The extent to which Long Interspersed Element-1 (LINE-1, L1) ORF1p is expressed in cutaneous malignancies remains to be evaluated. This study aimed to assess LINE-1 ORF1p immunoreactivity in various skin cancer subtypes. Method: The expression level of LINE-1 ORF1p was evaluated in 95 skin cancer specimens comprising 36 (37.9) BCC, 28 (29.5) SCC, and 31 (32.6) melanoma using the tissue microarray (TMA) technique. Then the association between expression of LINE-1 encoded protein and clinicopathological parameters was analyzed. Results: We showed that LINE-1 ORF1p expression level was substantially higher in BCC and SCC patients compared with melanoma samples (p 0.05). Conclusions: According to our observation, LINE-1 ORF1p immunoreactivity in various skin tumor subtypes extends previous studies of LINE-1 expression in different cancers. LINE-1ORF1p overexpression in NMSCs compared with MM can be considered with caution as a tumor-specific antigen for NMSCs. © 2020 The Author(s)

Generating power and controversy: Understanding Tanzania’s independent power projects

Initially conceived of within the broader context of power sector reform in the late 1980s and early 1990s, Independent Power Projects (IPPs) were intended to relieve state utilities of the burden of financing new plants, bring quick, quality power and reduce costs for end-users. Although IPPs have indeed contributed to generation capacity in Tanzania, much of the power that resulted from investments has been supplied neither quickly nor cheaply. Embarking on power sector reform in the early 1990s, Tanzania made IPPs a pillar of its reform strategy. Presently, Songas and IPTL, the country’s two IPPs are helping to reduce load shedding. However, these projects have not been without controversy. One of Tanzania’s IPPs was taken to international arbitration over a dispute related to construction costs. The state electric utility, Tanzania Electric Supply Company Limited (TANESCO), currently pays more than 50% of its current revenue towards combined fuel and capacity charges for the IPPs. Capacity charges for the country’s two IPPs are equivalent to approximately one percent of GDP. The Government of Tanzania (GoT) is intervening to assist TANESCO with its monthly IPP payments at present. With twenty-year Power Purchase Agreements (PPAs) between IPPs and TANESCO, these costs are expected to continue, albeit with some modifications due to refinancing, fuel conversion and further development of the natural gas market. This paper provides a detailed summary of how and why IPPs developed in Tanzania as well as their impact to date. Development outcomes, namely the extent to which the host country is benefiting from reliable, affordable power and investment outcomes, the degree to which investors have made favourable returns and been able to expand market share, are analysed in turn. IPPs offer more than a decade of experiences in private sector investment in developing countries and a detailed understanding of them may be the key to unlocking and sustaining future power investment

Generating power and controversy: Understanding Tanzania’s independent power projects

Initially conceived of within the broader context of power sector reform in the late 1980s and early 1990s, Independent Power Projects (IPPs) were intended to relieve state utilities of the burden of financing new plants, bring quick, quality power and reduce costs for end-users. Although IPPs have indeed contributed to generation capacity in Tanzania, much of the power that resulted from investments has been supplied neither quickly nor cheaply. Embarking on power sector reform in the early 1990s, Tanzania made IPPs a pillar of its reform strategy. Presently, Songas and IPTL, the country’s two IPPs are helping to reduce load shedding. However, these projects have not been without controversy. One of Tanzania’s IPPs was taken to international arbitration over a dispute related to construction costs. The state electric utility, Tanzania Electric Supply Company Limited (TANESCO), currently pays more than 50% of its current revenue towards combined fuel and capacity charges for the IPPs. Capacity charges for the country’s two IPPs are equivalent to approximately one percent of GDP. The Government of Tanzania (GoT) is intervening to assist TANESCO with its monthly IPP payments at present. With twenty-year Power Purchase Agreements (PPAs) between IPPs and TANESCO, these costs are expected to continue, albeit with some modifications due to refinancing, fuel conversion and further development of the natural gas market. This paper provides a detailed summary of how and why IPPs developed in Tanzania as well as their impact to date. Development outcomes, namely the extent to which the host country is benefiting from reliable, affordable power and investment outcomes, the degree to which investors have made favourable returns and been able to expand market share, are analysed in turn. IPPs offer more than a decade of experiences in private sector investment in developing countries and a detailed understanding of them may be the key to unlocking and sustaining future power investment

Characteristics of mixed type basal cell carcinoma in comparison to other BCC subtypes

Background: There are limited data exploring the characteristics of mixed type basal cell carcinoma (BCC). Objectives: To explore different characteristics of mixed type BCC. Design: Cross sectional study. Materials and Methods: 825 patients with BCC enrolled in this study. Results: Among 825 patients, 512 (62%) were male. Three hundred and fifty five (43%) presented with nodular subtype, 267 (32.4%) with mixed subtype, 25 with superficial and the 178 remaining presented with other subtypes. Four hundred and eighty three (58.6%) of the lesions were on the face, 243 (29.5%) on scalp, 52 (6.3%) on ears, 20 (2.4%) on neck, 15 (1.8%) on trunk and 12 (1.4%) on extremities. Anatomic distribution of mixed type was as follows: 137 on face, (51.4%), 100 (37.3%) on scalp, 19 (7%) on ear, 6 (2.1%) on neck, 4 (1.5%) extremity and 1 (0.7%) on trunk, which the difference from non mixed types was statistically significant (P = 0.002). The mean diameter of the mixed types and non mixed type BCCs were significantly different (2.7 ± 2.1 cm vs. 2.2 ± 1.6 cm; P = 0.01. The prevalence of necrosis in mixed type BCC was two times higher than non mixed type BCCs (OR = 2.3, CI 95% 1.3-3.9, P = 0.001). The most frequent combined subtypes were nodular-infiltrative (P < 0.001). Conclusion: Mixed type BCC has differences with other BCC subtypes in anatomical distribution and tumor diameter. Indeed, mixed type BCCs are frequently composed of aggressive subtypes than nonaggressive subtypes