10 research outputs found
Dayside Field-Aligned Current Impacts on Ionospheric Irregularities
Global Navigation Satellite Systems (GNSS) are subject to disturbances caused by plasma irregularities in the ionosphere. Studies have suggested that in addition to the gradient drift and KelvinâHelmholtz instabilities, electron precipitation may be important for phase scintillations in the dayside auroral region. This study combines in situ Swarm data with ground GNSS observations to investigate the potential role of filamentary fieldâaligned currents (FACs) on phase scintillations in the dayside auroral region by analyzing 22 events with phase scintillations exceeding 0.45 radians. We observe colocation between regions of severe phase scintillations and highly filamented FACs with fluctuations measured in the spacecraft frame of the order of 20 Hz. The observations indicate that filamentary FACs are crucial drivers for irregularities responsible for creating severe phase scintillations measured in the dayside auroral region and are thus of significant importance in the context of space weather impact on satellite communication.publishedVersio
Dayside Field-Aligned Current Impacts on Ionospheric Irregularities
Global Navigation Satellite Systems (GNSS) are subject to disturbances caused by plasma irregularities in the ionosphere. Studies have suggested that in addition to the gradient drift and KelvinâHelmholtz instabilities, electron precipitation may be important for phase scintillations in the dayside auroral region. This study combines in situ Swarm data with ground GNSS observations to investigate the potential role of filamentary fieldâaligned currents (FACs) on phase scintillations in the dayside auroral region by analyzing 22 events with phase scintillations exceeding 0.45 radians. We observe colocation between regions of severe phase scintillations and highly filamented FACs with fluctuations measured in the spacecraft frame of the order of 20 Hz. The observations indicate that filamentary FACs are crucial drivers for irregularities responsible for creating severe phase scintillations measured in the dayside auroral region and are thus of significant importance in the context of space weather impact on satellite communication
Identification of Continuous B-Cell Epitopes on the Protein Moiety of the 58-Kilodalton Cell Wall Mannoprotein of Candida albicans Belonging to a Family of Immunodominant Fungal Antigens
The 58-kiloDalton mannoprotein (mp58) on the surface of Candida albicans is highly immunogenic, is expressed by all C. albicans isolates tested, and elicits strong antibody responses during candidiasis. It belongs to a family of immunodominant fungal antigens with representatives also in different species of Aspergillus. The amino acid sequence of the protein portion of mp58 as deduced from the DNA sequence of its encoding gene (FBP1/PRA1) was used to synthesize a complete set of overlapping dodecapeptides (overlap, 7; offset, 5) covalently attached to the surface of derivatized polyethylene pins. The pin-coupled peptides were used in a modified enzyme-linked immunosorbent assay (ELISA) to identify continuous epitopes recognized by a number of antiserum preparations containing anti-mp58 antibodies. This comprehensive epitope-scanning study revealed the presence of multiple immunoreactive continuous B-cell epitopes within the protein sequence. Regions of increased reactivity included both the amino and carboxy termini of the mature protein (encompassing amino acid residues 16 to 50 and 286 to 299, respectively) and four internal regions spanning amino acids at positions 66 to 92, 121 to 142, 148 to 192, and 211 to 232. Further delineation of epitopic regions and identification of the boundaries of the antigenic sites was performed upon ELISA testing with a second Pepset consisting of completely overlapping 8-mer peptides spanning these reactive regions in the protein moiety of mp58. The highly reactive epitopic region at the C terminus of the protein was further evaluated using both window net and replacement net analyses. A synthetic peptide corresponding to the last 10 amino acid residues at the C terminus of the protein was immunogenic when injected into mice after being coupled to a carrier protein. Moreover, antibodies in the resulting sera specifically recognized the homologus mp58 in ELISAs and immunoblot assays. Delineation of the antibody responses to mp58 could provide the basis for the development of novel immunity-based prophylactic, therapeutic, and diagnostic techniques for the management of candidiasis