Thyroid Transcription Factor-1 Facilitates Cerebrospinal Fluid Formation by Regulating Aquaporin-1 Synthesis in the Brain

In the brain, aquaporin-1 (AQP-1), a water channel for high osmotic water permeability, is mainly expressed in the apical membrane of the ventricular choroid plexus and regulates formation of cerebrospinal fluid (CSF). Although the physiology of AQP-1 has been the subject of several publications, much less is known about the trans-acting factors involved in the control of AQP-1 gene expression. Here we report that TTF-1, a homeodomain-containing transcriptional regulator, is coexpressed with AQP-1 in the rat brain choroid plexus and enhances AQP-1 gene transcription by binding to conserved core TTF-1-binding motifs in the 5'-flanking region of the AQP-1 gene. Intracerebroventricular administration of an antisense TTF-1 oligodeoxynucleotide significantly decreased AQP-1 synthesis and reduced CSF formation. In addition, blockade of TTF-1 synthesis increased survival of the animals following acute water intoxication-induced brain edema. These results suggest that TTF-1 is physiologically involved in the transcriptional control of AQP-1, which is required for CSF formation

Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues

Abstract Background The incidence of inflammatory bowel disease (IBD), an inflammatory disorder of the gastrointestinal system has increased. IBD, characterized by aberrant immune responses against antigens, is thought to be caused by the invasion of enterobacteria. The pathogenesis of IBD is complicated, hence novel effective therapeutic agents are warranted. Therefore, this study evaluates the potential of Artemisia argyi, a medicinal herb, in alleviating IBD. Methods The effectiveness of the A. argyi ethanol extract was verified both in vitro and in vivo. Inflammation was induced in RAW 264.7 cells by 1μg/mL of lipopolysaccharide (LPS) and by3% dextran sodium sulfate(DSS) in a DSS-induced colitis mouse model. During the ten-day colitis induction, 200mg/kg of A. argyi ethanol extract was orally administered to the treatment group. Levels of inflammation-related proteins and genes were analyzed in the colon, serum, and lymphoid tissues, i.e., Peyers patches (PPs) and spleen. The chemical constituent of the A. argyi ethanol extract was identified using an ultra-high performance liquid chromatography mass spectrometry (UPLC-MS/MS) analysis. Results A. argyi ethanol extract treatment ameliorated IBD symptoms and reduced the expression of inflammation-related proteins and genes in the colon and serum samples. Furthermore, A. argyi treatment induced the activation of anti-oxidative associated proteins, such as nuclear factor-erythroid factor 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1); and the treatment have also inhibited nuclear factor-κB (NF-κB), a central mediator of inflammatory responses. A. argyi enhanced the immunomodulatory effects in the PPs and spleen, which may stem from interleukin-10 (IL-10) upregulation. Chemical analysis identified a total of 28 chemical compounds, several of which have been reported to exert anti-inflammatory effects. Conclusions The effectiveness of the A. argyi ethanol extract in alleviating IBD was demonstrated; application of the extract successfully mitigated IBD symptoms, and enhanced immunomodulatory responses in lymphoid tissues. These findings suggest A. argyi as a promising herbal medicine for IBD treatment

TTF-1 Action on the Transcriptional Regulation of Cyclooxygenase-2 Gene in the Rat Brain

We have recently found that thyroid transcription factor-1 (TTF-1), a homeodomain-containing transcription factor, is postnatally expressed in discrete areas of the hypothalamus and closely involved in neuroendocrine functions. We now report that transcription of cyclooxygenase-2 (COX-2), the rate limiting enzyme in prostaglandin biosynthesis, was inhibited by TTF-1. Double immunohistochemistry demonstrated that TTF-1 was expressed in the astrocytes and endothelial cells of blood vessel in the hypothalamus. Promoter assays and electrophoretic mobility shift assays showed that TTF-1 inhibited COX-2 transcription by binding to specific binding domains in the COX-2 promoter. Furthermore, blocking TTF-1 synthesis by intracerebroventricular injection of an antisense oligomer induced an increase of COX-2 synthesis in non-neuronal cells of the rat hypothalamus, and resulted in animals' hyperthermia. These results suggest that TTF-1 is physiologically involved in the control of thermogenesis by regulating COX-2 transcription in the brain

Development of Equipment and Application of Machine Learning Techniques Using Frequency Response Data for Cap Damage Detection of Porcelain Insulators

The most common method for inspection of insulators is to measure the change of electrical characteristics such as electric resistance and partial discharge. However, even if there is no physical damage, these values vary depending on the temperature, humidity, and chloride content of the atmosphere. In this respect, an alternative to such methods can be the impact response test, and a frequency response function (FRF) obtained from the test has been widely used as a tool for damage detection. In this study the FRF was applied to identify the cap damage of porcelain insulators. In addition, to solve the danger of high voltage and poor field accessibility near the insulator, a device with high field applicability was developed to measure FRF from a long distance using an auto impact hammer and Micro Electro Mechanical Systems (MEMS) technology. Even though the FRF is most suitable for inspection of porcelain insulators, dynamic characteristics such as natural frequencies may vary depending on manufacturing errors, installation conditions, etc., which may cause difficulties in damage identification. To overcome this limitation, the machine learning (ML) method was applied in this study to provide a diagnostic method that ensured consistent and accurate judgment. As a result of predicting the normal and the cap damage data using the support vector machine (SVM), bagging, k-nearest neighbor (kNN), and discriminant analysis (DA) methods, the overall F1 score was over 87% and the bagging method achieved the highest accuracy. In this study, the frequency range and dynamic characteristics that are sensitive to the physical damage of the insulator were derived and, based on this, the optimum ML methods with improved equipment could provide analysis with higher accuracy and consistency than general analysis using the FRF

Performance Characteristics of a Seawater Ice-Making Device Using a Scraped Surface Double Tube Evaporator

In this study, to investigate the performance characteristics of a seawater ice-making device, using a scraped surface double tube evaporator, experiments were conducted under various operating conditions, including inlet temperature and flow rate of seawater, evaporating temperature, and scraper rotation speed. The main results are summarized as follows: (1) The section where stable ice making is possible, is determined by the inlet temperature and the flow rate range of seawater. By controlling the flow rate of seawater, the ice packing factor (IPF) of the device can be adjusted from 0.3 to 14.7%. (2) The IPF increases in cases where the evaporating temperature decreases linearly, until −13 °C. As the temperature of refrigerant that flows into the evaporator changes, the IPF is changed. Consideration is required for these connections. (3) The IPF increases when speeding up the scraper. The minimum rotating speed of the scraper was 350 rpm in these experiments. Optimum operating conditions for the seawater ice slurry maker are established through experiments. These results will be considered as important data for designing a slurry type seawater ice-making device

Influence of stacking disorder on cross-plane thermal transport properties in TMPS3 (TM = Mn, Ni, Fe)

© 2020 Author(s). We investigated the thermal transport properties of magnetic van der Waals materials, TMPS3 (TM = Mn, Ni, and Fe), using the time-domain thermoreflectance technique. We determined the cross-plane thermal conductivity, which turns out to be relatively low, i.e., about 1W m(-1) K-1 for all TMPS3 investigated. When compared with previous results of graphite and transition metal dichalcogenides (TMDs), thermal conductivity becomes smaller as it goes from graphite to TMDs to TMPS3, and the difference is larger at low temperature, e.g., around 50K. From the Callaway model analysis, we could attribute the large thermal conductivity reduction for TMPS3, particularly at low temperature, to the phonon scattering from the boundary. We actually confirmed the existence of the large population of the stacking faults with the cross-sectional transmission electron microscopy image of MnPS3. This suggests that intrinsic or extrinsic stacking faults formed in van der Waals materials and their heterostructures can play an important role in reducing the cross-plane thermal conductivity as a source of the boundary scattering11sci

Influence of stacking disorder on cross-plane thermal transport properties in TM

© 2020 Author(s). We investigated the thermal transport properties of magnetic van der Waals materials, TMPS3 (TM = Mn, Ni, and Fe), using the time-domain thermoreflectance technique. We determined the cross-plane thermal conductivity, which turns out to be relatively low, i.e., about 1W m(-1) K-1 for all TMPS3 investigated. When compared with previous results of graphite and transition metal dichalcogenides (TMDs), thermal conductivity becomes smaller as it goes from graphite to TMDs to TMPS3, and the difference is larger at low temperature, e.g., around 50K. From the Callaway model analysis, we could attribute the large thermal conductivity reduction for TMPS3, particularly at low temperature, to the phonon scattering from the boundary. We actually confirmed the existence of the large population of the stacking faults with the cross-sectional transmission electron microscopy image of MnPS3. This suggests that intrinsic or extrinsic stacking faults formed in van der Waals materials and their heterostructures can play an important role in reducing the cross-plane thermal conductivity as a source of the boundary scattering11sci