Relapsing Brevibacterium casei Peritonitis: Value of 16S rRNA Gene Sequencing in Accurate Species Identification

status: publishe

A Case of IgG4-Related Sclerosing Disease With Retroperitoneal Fibrosis, Autoimmune Pancreatitis and Bilateral Focal Nephritis

A 74-year-old male patient presented with progressive anorexia, cholestatic liver function tests, and a diffuse enlarged pancreas suggestive of a pancreatic carcinoma. There was a marked elevation of total immunoglobulin G4 (IgG4) in serum. Further investigation led to the diagnosis of IgG4-related sclerosing disease with involvement of the pancreas, retroperitoneal fibrosis, and bilateral focal nephritis. To our knowledge, this is the first report on these 3 clinical entities occurring in the same patient.A short review of the literature concerning autoimmune pancreatitis and retroperitoneal fibrosis is made, with special interest to the concept of IgG4-related pathology. This systemic disease can have several clinical manifestations: IgG4-positivity not only can be found in the pancreas, but also at the level of extrahepatic biliary ducts, gallbladder, salivary glands, retroperitoneal tissue, kidneys, ureters, and lymph nodes. Although further investigation is required to determine its exact pathophysiologic role, IgG4 seems to be an important key player.status: publishe

Autoimmune pancreatitis and extrapancreatic manifestations of IgG4-related sclerosing disease

In a review of the literature concerning autoimmune pancreatitis we had special interest for the concept of IgG4-related pathology as a systemic disease with several clinical manifestations. In general, IgG4-positivity can not only be found in the pancreas, but also at the level of the kidneys, extrahepatic biliary ducts, gallbladder, lungs, salivary glands, lacrimal glands, retroperitoneal tissue, ureters, prostate, meninges and lymph nodes. IgG4 seems to be a central key player in the pathophysiology of this disease.status: publishe

Achtergronddocument 2007, Thema Bodem - Milieurapport Vlaanderen, MIRA (2006)

nrpages: 165status: publishe

Reference materials and representative test materials to develop nanoparticle characterization methods: the NanoChOp project case

This paper illustrates how to use generic guidelines on the production of reference materials (RMs) for the selection and characterization of a common set of nanoparticle test materials for a collaborative research project on techniques to assess nanoparticles in biologically relevant media. It addresses the challenges related to the production of suspensions of nanoparticles with defined surface groups and monomodal size distributions, in casu suspensions of silica and titanium dioxide nanoparticles and CdSe/CdS/ZnS quantum dots. Several candidate materials were investigated with small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and centrifugal liquid sedimentation (CLS) to establish whether they complied with the required monomodal particle size distribution. The unwanted presence of impurities, aggregates, agglomerates and viable microorganisms in the suspensions was investigated with DLS, CLS, optical and electron microscopy and via plating on nutrient agar. Suitability of the surface functionalization was investigated bymeasuring the capacity of the nanoparticles to be fluorescently labeled or to bind antibodies. For the three selected materials (aqueous suspensions of silica, aminated silica and CdSe/CdS/ZnS quantum dots) between-unit homogeneity and stability were investigated in terms of particle size and zeta potential. This paper illustrates how the outcome of the characterization process defines the status of the selected suspensions as 'representative test material' (recently defined in ISO TS 16195:2013) or as'reference material' (ISO Guide 30:2015), and how this status depends on their intended use. A range of practical tips for the processing of nanoparticle suspensions is offered, as well as conceptual guidance for the production of representative test materials and reference materials used in collaborative research projects on nanoparticle characterization and in other areas of measurement 43 science.JRC.D.2-Standards for Innovation and sustainable Developmen