Analysis of Microsatellite Polymorphism in Inbred Knockout Mice

Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically modified inbred mouse strain. In this study, we further examined whether CMP also relates to the homologous recombination in gene knockout (KO) mouse strains. The same 42 microsatellite loci were analyzed by polymerase chain reaction (PCR) in 29 KO inbred mouse strains via short tandem sequence repeat (STR) scanning and direct sequence cloning to justify microsatellite polymorphisms. The C57BL/6J and 129 mouse strains, from which these 29 KO mice were derived, were chosen as the background controls. The results indicated that 10 out of 42 (23.8%) loci showed CMP in some of these mouse strains. Except for the trinucleotide repeat locus of D3Mit22, which had microsatellite CMP in strain number 9, the core sequences of the remaining 41 loci were dinucleotide repeats, and 9 out of 41 (21.95%) showed CMPs among detected mouse strains. However, 11 out of 29 (37.9%) KO mice strains were recognized as having CMPs. The popular dinucleotide motifs in CMP were (TG)n (50%, 2/4), followed by (GT)n (27.27%, 3/11) and (CA)n (23.08%, 3/13). The microsatellite CMP in (CT)n and (AG)n repeats were 20% (1/5). According to cloning sequencing results, 6 KO mouse strains showed insertions of nucleotides whereas 1 showed a deletion. Furthermore, 2 loci (D13Mit3 and D14Mit102) revealed CMP in 2 strains, and mouse strain number 9 showed CMPs in two loci (D3Mit22 and D13Mit3) simultaneously. Collectively, these results indicated that microsatellite polymorphisms were present in the examined inbred KO mice

'You couldn't say "No", Could you?': Young men's understandings of sexual refusal

While several psychological theories of rape have been developed, Tannen's ‘ miscommunication’ model is dominant, informing ‘expert’ and popular accounts alike. Rape is constructed as an extreme example of miscommunication – whereby women's ‘failure’ to say ‘no’ is interpreted by men as sexual consent. Kitzinger and Frith have demonstrated that young women have an implicit understanding of the normative interactional structure of refusal, and it is this that explains their difficulty in ‘just saying no’ to unwanted sex. However, Kitzinger and Frith's study could not demonstrate, but only argue, that young men share this sophisticated understanding, such that women saying ‘no’ should not be necessary to refuse sexual intimacy. Here we extend Kitzinger and Frith's study, via the analysis of data from two focus groups held with young men. We demonstrate that, as Kitzinger and Frith suggested, men not only do have a refined ability to hear verbal refusals that do not contain the word ‘no’, but also – and importantly – an equally refined ability to ‘hear’ the subtlest of non-verbal sexual refusals