Nanocapture 3.3.1

<p>This software package of Nanocapture 3.3.1 is made available for your convenience, since our AFS tracking software uses drivers from here.</p

Agilent 33XXX

<p>This software package of Agilent 33XXX series is made available for your convenience, since our AFS tracking software uses the vi's and drivers from here.</p

AFS software

<p>AFS software corresponding to the paper: "Acoustic Force Spectroscopy" in Nature Methods.</p

Thorlabs_uc480

<p>This software package of thorlabs uc480 is made available for your convenience, since our AFS tracking software uses the vi's and drivers from here.</p

AFS 1D model

<div>This software is divided in two parts: (i) an set of classes, methods and functions that allow for modelling the radiation force profile in a configuration of a transducer-resonator-flow cell, and (ii) a script that performs a massive automated sweep over some properties of this configuration, thus creating a parameter space for the radiation force. Both parts are equipped with a graphical user interface, the former for altering a configurations’ properties and analysing the effects in the model, the latter for exploring the plots of the parameter space, eg. in search for optimal configurations.</div

Imaging unlabeled proteins on DNA with super-resolution

Fluorescence microscopy is invaluable to a range of biomolecular analysis approaches. The required labeling of proteins of interest, however, can be challenging and potentially perturb biomolecular functionality as well as cause imaging artefacts and photo bleaching issues. Here, we introduce inverse (super-resolution) imaging of unlabeled proteins bound to DNA. In this new method, we use DNA-binding fluorophores that transiently label bare DNA but not protein-bound DNA. In addition to demonstrating diffraction-limited inverse imaging, we show that inverse Binding-Activated Localization Microscopy or 'iBALM' can resolve biomolecular features smaller than the diffraction limit. The current detection limit is estimated to lie at features between 5 and 15 nm in size. Although the current image-acquisition times preclude super-resolving fast dynamics, we show that diffraction-limited inverse imaging can reveal molecular mobility at ∼0.2 s temporal resolution and that the method works both with DNA-intercalating and non-intercalating dyes. Our experiments show that such inverse imaging approaches are valuable additions to the single-molecule toolkit that relieve potential limitations posed by labeling