Functional abilities of cultivable plant growth promoting bacteria associated with wheat (Triticum aestivum L.) crops

Abstract In the pursuit of sustainable agriculture, bioinoculants usage as providers of a crop's needs is a method to limit environmental damage. In this study, a collection of cultivable putative plant growth promoting (PGP) bacteria associated with wheat crops was obtained and this bacterial sample was characterized in relation to the functional diversity of certain PGP features. The isolates were obtained through classical cultivation methods, identified by partial 16S rRNA gene sequencing and characterized for PGP traits of interest. Functional diversity characterization was performed using Categorical Principal Component Analysis (CatPCA) and Multiple Correspondence Analysis (MCA). The most abundant genera found among the 346 isolates were Pseudomonas, Burkholderia, and Enterobacter. Occurrence of PGP traits was affected by genus, niche, and sampling site. A large number of genera grouped together with the ability to produce indolic compounds; phosphate solubilization and siderophores production formed a second group related to fewer genera, in which the genus Burkholderia has a great importance. The results obtained may help future studies aiming prospection of putative plant growth promoting bacteria regarding the desired organism and PGP trait

Degradation of chlorinated benzoic acid mixtures by plant-bacteria associations

NRC publication: Ye

Responses of arbuscular mycorrhizal fungal communities to soil core transplantation across Saskatchewan prairie climatic regions

No information exists on the susceptibility of arbuscular mycorrhizal fungal (AMF) communities in Canadian prairie agriculture soils to climate change. An experiment was initiated in mid-May 2011 in which replicated soil cores were transplanted reciprocally from four cultivated prairie sites in Saskatchewan, Canada representing different regional climatic zones ranging from semi-arid to sub-humid regional climates, such that replicated (n=4) soil cores from each site were present at all sites. Field pea was grown in all cores and at harvest in early-September 2011, soil samples were collected to analyze the changes of AMF communities over the cropping season. A total of 82 operational taxonomic units belonging to eight AMF genera were identified using 18S rRNA gene pyrosequencing. When soils were transplanted to new environments, the relative abundance of AMF changed considerably. Typically, Shannon diversity declined when soil cores were transplanted to new environments. We present evidence that the altered climatic conditions following transplantation of soil cores, the relative abundance of AMF was significantly altered, and some taxa were enhanced, suppressed or disappeared in the home-away soils, compared to home-site soils. This study implies the future climate change effects on AMF may impact specific phylogenetic taxa differently, such that rare species or those with low abundance may increase or decrease with unknown consequences. Understanding the potential responses of AMF communities to soil-climate interactions is important when considering the impacts of climate change on soil microbial communities.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author