Phytochemistry investigation and antioxidant activity of four edible Verbenaceae of Burkina Faso

Lantana rhodesiensis Moldenke, Lippia chevalieri Moldenke, Vitex diversifolia Bak and Vitex doniana Sweet are four species of Verbenaceae family widely used in traditional medicines in Burkina. The aim of this investigation was to study the chromatographic profile of phenols acids and that of the flavonoids as well as the antioxidants activities. Then, three types of extraction were made: acetonic (100%), decoction, ethanol-water(80/20 + 20/80); and fractions of ethanol-water which arehexane fraction (HF), ethyl acetate fraction (EAF), butanol fraction (BF) and aqueous fraction (AqF) of each species. These evaluations were supplemented by the quantification of the metal ions and the characterization by atomic absorption and HPLC-DAD methods of totals phenols acids and totals flavonoids.The whole of these proportioning was directed against the antioxidant activity through the anti-DPPH* (free radical scavenging activity), anti-TAC (total antioxidant capacity) and anti-IRP(iron reducing power).36 flavonoids on 41 were highlighted by the HPLC-DAD method. A derivative glycosylate of quercetin was detected in the V. diversifolia extracts. L. chevalieri presented the strongest value in terms of ions content with a 55.88 g/kg of calcium.In terms of antioxidant activity, L. rhodesiensis and L. chevalieri presented the best activities on total antioxidant activity and anti-DPPH, and iron reducing power respectively.It’s arise that decoction extract have the best’s activities and can justify the traditional uses

ANTI-INFLAMMATORY ACTIVITIES OF FRUIT AND LEAVES EXTRACT OF LANNEA MICROCARPA ENGLAND K. KRAUS (ANACARDIACEAE)

Objective:  To investigate the anti-inflammatory activity as well as carotenoid content of crude hydro-acetonic extract of fruit and leaves of Lannea microcarpa (L. microcarpa) and to elucidate the possible anti-inflammatory mechanism by enzymatic and non-enzymatic methods.Methods:  Anti-inflammatory activity was determined by using carrageenan induced paw edema in mice;  the anti-inflammatory mechanism may have been assessed using the inhibitory effect of the extracts, on the lipoxygenase, xanthine oxidase, lipid peroxidation and the reduction of Fe3+.Results:  At the doses of 100mg/kg, 200mg/kg, 400mg/kg, the extracts of both fruit and leaves reduced the carrageenan-induced paw edema while the dose of 200 mg/kg has produced a maximum percentage of inhibition of mice paw edema both for fruit (78.44%) and leaves (58.02%) at the fifth hour compared to control. Significant lipoxigenase and xanthine oxidase inhibitory effect was obtained with both fruit and leaves extract ethyl acetate fraction. Ethyl acetate fraction from both extracts inhibited lipid peroxidation. The fruit and leaves extract ethyl acetate fraction also inhibited lipid peroxidation with 32.85% and 78.07% respectively. Crude acetonic extract of leaves of Lannea microcarpa has shown a significantly reducing power of Fe3+ by 9.46±0.26 and high carotenoids content compared to those of fruit extract.Conclusion: The results obtained from the present study suggest that Lannea microcarpa fruit and leaves extract possess significant anti-inflammatory and antioxidant capacities (mg AAE/100 mg). Therefore, they could be useful for food and pharmaceutics industries.Â

Carotenoids content and antibacterial activity from galls of Guiera senegalensis J.F. Gmel (combretaceae).

Guiera senegalensis is a well known medicinal plant which is used as a drug in Burkina Faso. The purpose of the present study was to investigate the carotenoids content and antibacterial activity from galls of Guiera senegalensis. The hydroacetonic extract (HAE) exhibited the highest of β-carotene (4.67±0.25 mg/g) and lycopene (0.99±0.0735 mg/g) content. The extracts and fractions of galls exhibited antimicrobial activity against some gram positive and gram negative bacteria. We observed an inhibitory activity of the extracts against Bacillus cereus ATCC 13061 and Staphylococus aureus ATCC 6538 which showed a resistance to the penicillin and ampicillin. The ratio of the MBC and the MIC showed that the hydroacetonic extract is bactericidal against all the bacterial strains used except for Proteus mirabilis ATCC 35659. The aqueous decoction extract is bactericidal against all the bacterial species tested. The present study thus suggested that galls from G senegalensis may be used as a new potential source of natural nutraceutical components

Antimicrobial activity of polyphenol-rich fractions from Sida alba L. (Malvaceae) against co-trimoxazol-resistant bacteria strains

<p>Abstract</p> <p>Background</p> <p>The increased resistance of microorganisms to the currently used antimicrobials has lead to the evaluation of other agents that might have antimicrobial activity. Medicinal plants are sources of phytochemicals which are able to initiate different biological activities including antimicrobials</p> <p>Materials and methods</p> <p><it>In vitro </it>antibacterial (MIC, MBC and time-kill studies) of polyphenol-rich fractions from <it>Sida alba </it>L. (Malvaceae) was assessed using ten bacteria strains (Gram-negative and Gram-positive).</p> <p>Results</p> <p>All test bacteria were susceptible to the polyphenol-rich fractions. Time-kill results showed that after 5 h exposition there was no viable microorganism in the initial inoculum and the effect of polyphenol-rich fractions was faster on <it>Enterococcus faecalis </it>(Gram-positive bacterium) comparatively to the other bacteria strains.</p> <p>Conclusion</p> <p>The data analysis indicates that the tested of polyphenol-rich fractions has significant effects when compared with the standard antibiotic. These results therefore justify the traditional use of <it>sida alba </it>L., alone or in combination with other herbs to treat bacterial infections.</p

Antioxidant and Anti-Inflammatory Activities of Fractions from Bidens engleri O.E. Schulz (Asteraceae) and Boerhavia erecta L. (Nyctaginaceae)

Background: According to recent studies, reactive oxygen is the leader of human metabolic disease development. The use of natural antioxidants is the best way to stop or prevent this problem. Therefore, the aim of this study was to evaluate the antioxidant and anti-inflammatory activities and to determine the polyphenolic contents of the Bidens engleri and Boerhavia erecta fractions. Methods: Plant fractions were obtained using Soxhlet procedures with hexane, dichloromethane, acetonitrile, ethyl acetate, methanol, and butanol solvent, successively. The different fractions were compared according to their antioxidant, anti-inflammatory activities, total phenolic, and total flavonoid contents. The phenolic contribution to the biological activity was evaluated. Result: The Bidens engleri and Boerhavia erecta fractions showed the highest antioxidant abilities, notably the polar fractions, which inhibited significantly the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-O-azinobis(3-ethylbenzoline-6-sulphonate) (ABTS). The butanol fraction from Bidens engleri and methanol fraction from Boerhavia erecta have presented the best iron (III) reduction power with 211.68 and 198.55 mgAAE/g, respectively. Butanol and acetonitrile were the best solvents for extracting phenolic compounds from Bidens engleri and Boerhavia erecta, respectively. In contrast, dichloromethane was the best solvent for extracting a flavonoid from two plants with anti-COX-2 and anti-LOX-15 active compounds. The phenolic compound contributed significantly to antioxidant activity (r &gt; 0.80). Conclusion: The Bidens engleri and Boerhavia erecta fractions possessed a potential antioxidant for fighting oxidative stress and helping to prevent diabetes, hypertension, and cardiovascular diseases. The uses of this plant could be promoted in Burkina Faso

Antioxidant and anticancer activities of polyphenolic compounds from three Acanthaceae medicinal species from Burkina Faso

Phytochemical screening and biological activities of methanol extracts from three Acanthaceae species namely Hygrophila auriculata (H. auriculata), Nelsonia canescens (N. canescens) and Peristrophe bicalyculata (P. bicalyculata), widely used in local ethnomedicine, were carried out. Phenolic content quantification indicated that H. auriculata extract possessed the highest polyphenolics content followed by N. canescens and P. bicalyculata. Biological activities assessment showed that H. auriculata extract has the best antioxidant (through DPPH, FRAP and ABTS methods) capacity. Acetylcholinesterase and Lipoxygenase inhibition tests revealed interesting activities from P. bicalyculata. Testing cancer cells antiproliferation, it was showed that H. auriculata and N. canescens) were of good interest (27.00±1.55 and 29.70±3.11 µg/mL).These results showed that the phenolic compounds of these plants could justify their local traditional use to treat inflammatory and tumoral diseases

Phytochemistry investigation and antioxidant activity of four edible Verbenaceae of Burkina Faso

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Anti-Inflammatory, Analgesic and Antipyretic Effects of Lepidagathis Anobrya Nees (Acanthaceae)

This study investigated the general acute, anti-inflammatory, analgesic and antipyretic effects of methanol extract of Lepidagathis anobrya Nees (Acanthaceae). Carrageenan-induced rat paw edema and croton oil-induced ear edema in rats were used for the evaluation of general acute anti-inflammatory effects. Acetic acid-induced writhing response and yeast-induced hyperpyrexia in mice were used to evaluate the analgesic and antipyretic activities respectively. The extract at doses of 10, 25, 50 and 100 mgkg−1 for carrageenan test and doses of 0.5 mg/ear for croton oil test induced a significant reduction (p < 0.001) of paw and ear edemas in rats. In the analgesic and antipyretic tests, the extract has shown a significant inhibition of writhes and hyperpyrexia with all the doses used when compared to the untreated control group. These results clearly show the anti-inflammatory, analgesic and antipyretic effects of the methanol extract of Lepidagathis anobrya and give the scientific basis for its traditional use. Further studies are needed to clarify the mechanism of action and the components responsible for these pharmacological effects

Anti-Inflammatory, Analgesic And Antipyretic Effects Of Lepidagathis Anobrya Nees (Acanthaceae)

This study investigated the general acute, anti-inflammatory, analgesic and antipyretic effects of methanol extract of Lepidagathis anobrya Nees (Acanthaceae). Carrageenan-induced rat paw edema and croton oil-induced ear edema in rats were used for the evaluation of general acute anti-inflammatory effects. Acetic acid- induced writhing response and yeast-induced hyperpyrexia in mice were used to evaluate the analgesic and antipyretic activities respectively. The extract at doses of 10, 25, 50 and 100 mgkg-1 for carrageenan test and doses of 0.5 mg/ear for croton oil test induced a significant reduction (p < 0.001) of paw and eat edemas in rats. In the analgesic and antipyretic tests, the extract has shown a significant inhibition of writhes and hyperpyrexia with all the doses used when compared to the untreated control group. These results clearly show the anti-inflammatory, analgesic and antipyretic effects of the methanol extract of Lepidagathis anobyra and give the scientific basis for its traditional use. Further studies are needed to clarify the mechanism of action and the components responsible for these pharmacological effects

A review on Lantana rhodesiensis Moldenke: traditional uses, phytochemical constituents and pharmacological activities

Lantana rhodesiensis Moldenke an herbaceous, belonging to the Verbenaceae family is widely used in Africa folk medicine for the treatment of cancer, measles, malaria, smallpox, strength, coughs, fever, rheumatism, body pains, diabetes mellitus arrhythmia, parasitic diseases, arterial hypertension, old diarrhoeas. Many studies have been conducted on the chemical composition of the whole plant of L. rhodesiensis as well as biological activities.  The aim of the present review was to give a detailed literature survey on its traditional uses, phytochemistry and therapeutical properties of L. rhodesiensis