Qualitative analysis about the experience of VPN from people with software expertise in Sweden

VPN is primarily used to encrypt your network traffic and identity online securely from a private location. This can be used as a safety measure to prevent theft of personal data. It also allows its user to change the geolocation to wherever they want which unlocks the possibility to use another country's services. Related work has shown that there are also downsides to using VPN services. Some VPN solutions do have security problems that its user could be unaware of. This study explored the experience and beliefs surrounding the usage of VPN while browsing the internet from people with software expertise. Interviews were conducted with people in different areas surrounding usage of VPN services to get a deeper understanding of why VPN is used and to what extent they believe VPN is providing anonymity and security of their data. The findings from this study is that the main reason to use a VPN is to access unavailable services. These services can vary from content online that is not available in the region from where you access the internet to services that are work related and locked to specific networks. Another finding was also that among these people the belief that the use of a VPN was enough to make a user anonymous by itself is controversial

Qualitative analysis about the experience of VPN from people with software expertise in Sweden

VPN is primarily used to encrypt your network traffic and identity online securely from a private location. This can be used as a safety measure to prevent theft of personal data. It also allows its user to change the geolocation to wherever they want which unlocks the possibility to use another country's services. Related work has shown that there are also downsides to using VPN services. Some VPN solutions do have security problems that its user could be unaware of. This study explored the experience and beliefs surrounding the usage of VPN while browsing the internet from people with software expertise. Interviews were conducted with people in different areas surrounding usage of VPN services to get a deeper understanding of why VPN is used and to what extent they believe VPN is providing anonymity and security of their data. The findings from this study is that the main reason to use a VPN is to access unavailable services. These services can vary from content online that is not available in the region from where you access the internet to services that are work related and locked to specific networks. Another finding was also that among these people the belief that the use of a VPN was enough to make a user anonymous by itself is controversial

Qualitative analysis about the experience of VPN from people with software expertise in Sweden

VPN is primarily used to encrypt your network traffic and identity online securely from a private location. This can be used as a safety measure to prevent theft of personal data. It also allows its user to change the geolocation to wherever they want which unlocks the possibility to use another country's services. Related work has shown that there are also downsides to using VPN services. Some VPN solutions do have security problems that its user could be unaware of. This study explored the experience and beliefs surrounding the usage of VPN while browsing the internet from people with software expertise. Interviews were conducted with people in different areas surrounding usage of VPN services to get a deeper understanding of why VPN is used and to what extent they believe VPN is providing anonymity and security of their data. The findings from this study is that the main reason to use a VPN is to access unavailable services. These services can vary from content online that is not available in the region from where you access the internet to services that are work related and locked to specific networks. Another finding was also that among these people the belief that the use of a VPN was enough to make a user anonymous by itself is controversial

Qualitative analysis about the experience of VPN from people with software expertise in Sweden

VPN is primarily used to encrypt your network traffic and identity online securely from a private location. This can be used as a safety measure to prevent theft of personal data. It also allows its user to change the geolocation to wherever they want which unlocks the possibility to use another country's services. Related work has shown that there are also downsides to using VPN services. Some VPN solutions do have security problems that its user could be unaware of. This study explored the experience and beliefs surrounding the usage of VPN while browsing the internet from people with software expertise. Interviews were conducted with people in different areas surrounding usage of VPN services to get a deeper understanding of why VPN is used and to what extent they believe VPN is providing anonymity and security of their data. The findings from this study is that the main reason to use a VPN is to access unavailable services. These services can vary from content online that is not available in the region from where you access the internet to services that are work related and locked to specific networks. Another finding was also that among these people the belief that the use of a VPN was enough to make a user anonymous by itself is controversial

Large Extracellular Vesicle Characterization and Association with Circulating Tumor Cells in Metastatic Castrate Resistant Prostate Cancer

Liquid biopsies hold potential as minimally invasive sources of tumor biomarkers for diagnosis, prognosis, therapy prediction or disease monitoring. We present an approach for parallel single-object identification of circulating tumor cells (CTCs) and tumor-derived large extracellular vesicles (LEVs) based on automated high-resolution immunofluorescence followed by downstream multiplexed protein profiling. Identification of LEVs >6 µm in size and CTC enumeration was highly correlated, with LEVs being 1.9 times as frequent as CTCs, and additional LEVs were identified in 73% of CTC-negative liquid biopsy samples from metastatic castrate resistant prostate cancer. Imaging mass cytometry (IMC) revealed that 49% of cytokeratin (CK)-positive LEVs and CTCs were EpCAM-negative, while frequently carrying prostate cancer tumor markers including AR, PSA, and PSMA. HSPD1 was shown to be a specific biomarker for tumor derived circulating cells and LEVs. CTCs and LEVs could be discriminated based on size, morphology, DNA load and protein score but not by protein signatures. Protein profiles were overall heterogeneous, and clusters could be identified across object classes. Parallel analysis of CTCs and LEVs confers increased sensitivity for liquid biopsies and expanded specificity with downstream characterization. Combined, it raises the possibility of a more comprehensive assessment of the disease state for precise diagnosis and monitoring

Long-term neurological effects of neonatal caffeine treatment in a rabbit model of preterm birth

Background: Neonatal caffeine treatment might affect brain development. Long-term studies show conflicting results on brain-related outcomes. Herein we aimed to investigate the long-term effects of neonatal caffeine administration in a rabbit model of preterm birth. Methods: Preterm (born day 29) and term (day 32) pups were raised by wet nurses and allocated to treatment with saline or caffeine for 7 or 17 days. At pre-puberty, neurobehavioral tests were performed and brains were harvested for immunostaining of neurons, synapses, myelin, and astrocytes. Results: Survival was lower in preterm saline pups than in controls, whereas caffeine-treated preterm pups did not differ from term control pups. Preterm saline pups covered less distance compared to controls and were more likely to stay in the peripheral zone of the open field. Corresponding differences were not seen in preterm caffeine pups. Preterm animals had lower neuron density compared to controls, which was not influenced by caffeine treatment. Synaptic density, astrocytes, and myelin were not different between groups. Conclusion: Caffeine appeared to be safe. All preterm rabbits had lower neuron density but anxious behavior seen in preterm saline rabbits was not seen in caffeine-treated preterm pups

Severe intraventricular hemorrhage causes long-lasting structural damage in a preterm rabbit pup model

Background: Intraventricular hemorrhage causes significant lifelong mortality and morbidity, especially in preterm born infants. Progress in finding an effective therapy is stymied by a lack of preterm animal models with long-term follow-up. This study addresses this unmet need, using an established model of preterm rabbit IVH and analyzing outcomes out to 1 month of age. Methods: Rabbit pups were delivered preterm and administered intraperitoneal injection of glycerol at 3 h of life and approximately 58% developed IVH. Neurobehavioral assessment was performed at 1 month of age followed by immunohistochemical labeling of epitopes for neurons, synapses, myelination, and interneurons, analyzed by means of digital quantitation and assessed via two-way ANOVA or Student’s t test. Results: IVH pups had globally reduced myelin content, an aberrant cortical myelination microstructure, and thinner upper cortical layers (I–III). We also observed a lower number of parvalbumin (PV)-positive interneurons in deeper cortical layers (IV–VI) in IVH animals and reduced numbers of neurons, synapses, and microglia. However, there were no discernable changes in behaviors. Conclusions: We have established in this preterm pup model that long-term changes after IVH include significant wide-ranging alterations to cortical organization and microstructure. Further work to improve the sensitivity of neurocognitive testing in this species at this age may be required. Impact: This study uses an established animal model of preterm birth, in which the rabbit pups are truly born preterm, with reduced organ maturation and deprivation of maternally supplied trophic factors.This is the first study in preterm rabbits that explores the impacts of severe intraventricular hemorrhage beyond 14 days, out to 1 month of age.Our finding of persisting but subtle global changes including brain white and gray matter will have impact on our understanding of the best path for therapy design and interventions