Resolving the Ripples (and a Mine): High-Resolution Multibeam Survey of Martha\u27s Vineyard ONR Mine Burial Program Field Area

In an effort to better understand the coastal processes responsible for the burial and exposure of small objects on the seafloor, the Office of Naval Research is sponsoring the Mine Burial Program. Among the field areas chosen for this program is the site of the Martha\u27s Vineyard Coastal Observatory (MVCO), a permanent instrumented node in 12 m of water about 500 m off the southern shore of Martha?s Vineyard. In support of the ONR program, several site surveys of the MVCO area have been conducted (see Goff et al); here we report the result of the most recent of these surveys, a very high-resolution multibeam survey aimed at establishing a detailed base map for the region and providing a baseline from which subsequent surveys can measure seafloor change In late July we conducted a five day survey of an approximately 3 x 5 km area surrounding the MVCO node using a Reson 8125 focused multibeam sonar aboard the SAIC survey vessel Ocean Explorer. The 8125 is a newly developed multibeam sonar that operates at 455 kHz and uses dynamic focusing to compensate for the curvature of the wavefront in the near-field. By using a relatively long array, the system can achieve very high spatial resolution (0.5 degree beam width) and with the dynamic focusing, can operate in the near field. The real constraint on resolution using this system is the ability to position the soundings and thus three kinematic DGPS base stations were established on Martha?s Vineyard and three kinematic receivers were used on the survey vessel. The kinematic GPS positioning is also critical to the ability to do repeat surveys with an accuracy high enough to resolve small (less than 10 cm) seafloor changes. Also to aid in our ability to accurately position repeat surveys, divers jetted sonar reflectors into the seafloor to act as fiducials. A super high-resolution (4 m overlap) survey was conducted in a small area surrounding the MVCO node and mine burial sites, a slightly lower resolution survey (12 to 25 m overlap) in a box approximately 1 x 1 km surrounding the ?target box? and a lower resolution survey (25 to 40 m line overlap) in a 3 x 5 km region surrounding the 1 x 1 km box. The Reson 8125 produced approximately 1 gigabyte of data per hour. The bathymetric resolution we were able to achieve was beyond our expectations. The node site and all diver-emplaced reflectors were clearly identified Most amazingly, we are able to resolve fields of individual ripples that are less than 2 cm height. Of particular relevance to the mine burial program was our ability to resolve an instrumented mine that had been deployed earlier by NRL. This mine is buried in a scour depression and is only a few centimeters proud above the base of the depression

Sec1/Munc18 Protein Stabilizes Fusion-Competent Syntaxin for Membrane Fusion in Arabidopsis Cytokinesis

SummaryIntracellular membrane fusion requires complexes of syntaxins with other SNARE proteins and regulatory Sec1/Munc18 (SM) proteins. In membrane fusion mediating, e.g., neurotransmitter release or glucose-stimulated insulin secretion in mammals, SM proteins preferentially interact with the inactive closed, rather than the active open, conformation of syntaxin or with the assembled SNARE complex. Other membrane fusion processes such as vacuolar fusion in yeast involve like membranes carrying cis-SNARE complexes, and the role of SM protein is unknown. We investigated syntaxin-SM protein interaction in membrane fusion of Arabidopsis cytokinesis, which involves cytokinesis-specific syntaxin KNOLLE and SM protein KEULE. KEULE interacted with an open conformation of KNOLLE that complemented both knolle and keule mutants. This interaction occurred at the cell division plane and required the KNOLLE linker sequence between helix Hc and SNARE domain. Our results suggest that in cytokinesis, SM protein stabilizes the fusion-competent open form of syntaxin, thereby promoting trans-SNARE complex formation

Congenital myelomeningocele - do we have to change our management?

<p>Abstract</p> <p>Background</p> <p>Eagerly awaiting the results of the Management of Myelomeningocele Study (MOMS) and with an increasing interest in setting up intrauterine myelomeningocele repair (IUMR), the optimal management of patients suffering from congenital myelomeningocele (MMC) has become a matter of debate again. We performed a cross-sectional study at our referral-center for MMC to determine the outcome for our expectantly managed patients.</p> <p>Materials and methods</p> <p>A computed chart review at our institution revealed 70 patients suffering from MMC. Forty-three patients were eligible for the study and analyzed further. A retrospective analysis was performed only in patients that underwent MMC repair within the first two days of life and were seen at our outpatient clinic between 2008 and 2009 for a regular multidisciplinary follow-up. Data were collected on: gestational age (GA) and weight at birth, age at shunt placement and shunt status after the first year of life, radiological evidence for Arnold-Chiari malformation (ACM) and tethered cord (TC), need for surgery for TC, bladder function, lower leg function and educational level. Data were compared to published results for IUMR and to studies of historical controls.</p> <p>Results</p> <p>Patients were born with MMC between 1979 and 2009 and are now 13.3 ± 8.9 (mean ± SD) years of age. At birth, mean GA was 37.8 ± 2.3 weeks and mean weight was 2921.3 ± 760.3 g, both significantly higher than in IUMR patients. Shunt placement in our cohort was required in 69.8% at a mean age of 16.0 ± 10.7 days, which was less frequent than for historical controls. Amongst our cohort, radiological observations showed 57.1% had ACM II and 41.9% had TC. Only two of our patients underwent a surgical correction for TC. Clean intermittent catheterization was performed in 69.7% of our patients, 56.4% were (assisted) walkers and 64.1% attended regular classes, both comparable to historical controls.</p> <p>Conclusions</p> <p>With a close and interdisciplinary management by pediatric surgeons, neurologists and urologists, the long-term outcome of patients suffering from MMC can currently be considered satisfactory. With respect to the known drawbacks of fetal interventions for mother and child, especially preterm delivery, the results of the MOMS trial should be awaited with caution before proceeding with a complex intervention like IUMR.</p

IL-2 Therapy Diminishes Renal Inflammation and the Activity of Kidney-Infiltrating CD4+ T Cells in Murine Lupus Nephritis

An acquired deficiency of interleukin-2 (IL-2) and related disturbances in regulatory T cell (Treg) homeostasis play an important role in the pathogenesis of systemic lupus erythematosus (SLE). Low-dose IL-2 therapy was shown to restore Treg homeostasis in patients with active SLE and its clinical efficacy is currently evaluated in clinical trials. Lupus nephritis (LN), a challenging organ manifestation in SLE, is characterized by the infiltration of pathogenic CD4+ T cells into the inflamed kidney. However, the role of the Treg-IL-2 axis in the pathogenesis of LN and the mode of action of IL-2 therapy in the inflamed kidneys are still poorly understood. Using the (NZB × NZW) F1 mouse model of SLE we studied whether intrarenal Treg are affected by a shortage of IL-2 in comparison with lymphatic organs and whether and how intrarenal T cells and renal inflammation can be influenced by IL-2 therapy. We found that intrarenal Treg show phenotypic signs that are reminiscent of IL-2 deprivation in parallel to a progressive hyperactivity of intrarenal conventional CD4+ T cells (Tcon). Short-term IL-2 treatment of mice with active LN induced an expansion the intrarenal Treg population whereas long-term IL-2 treatment reduced the activity and proliferation of intrarenal Tcon, which was accompanied by a clinical and histological amelioration of LN. The association of these immune pathologies with IL-2 deficiency and their reversibility by IL-2 therapy provides important rationales for an IL-2-based immunotherapy of LN.DFG, SFB 650, Zelluläre Ansätze zur Suppression unerwünschter Immunreaktionen - From Bench to Bedsid

The validation of the association between gene polymorphisms and the cytogenetic abnormalities frequency in the cohort of radiation facility employees

The results from the research into the association between polymorphisms of genes-candidates for individual radiosensitivity and the frequency and spectrum of cytogenetic abnormalities are analyzed. These polymorphisms have been previously identified in our microarray studies using “Cancer_SNP_Panel GT-17- 211” (“Illumina”, USA) in 2013. The study was conducted among Siberian Group of Chemical Enterprises healthy employees (n = 158) exposed to professional irradiation in a dose range of 100-300 mSv. We have found that 16 SNPs are associated with the frequency of dicentric and ring (the radiation exposure markers). We have found that 9 SNPs are confirmed to be associated with the frequency of dicentric (INSR rs1051690, TNKS rs33945943, CYP24A1 rs751087, GSK3B rs4624596, GSK3B rs4688046, GSK3B rs10934500, GSK3B rs1574154, GSK3B rs2873950, VCAM1 rs2392221) and 14 SNPs are confirmed to be associated with the frequency of ring (ESR1 rs488133, PIN1 rs889162, PIN1 rs2233679, CYP2С19 rs4986894, CYP24A1 rs751087, APAF1 rs2288729, MPDU1 rs4227, GSK3B rs4624596, GSK3B rs4688046, GSK3B rs10934500, GSK3B rs10934503, GSK3B rs1574154, GSK3B rs2873950, VCAM1 rs2392221)

Electronic Chart of the Future: The Hampton Roads Project

ECDIS is evolving from a two-dimensional static display of chart-related data to a decision support system capable of providing real-time or forecast information. While there may not be consensus on how this will occur, it is clear that to do this, ENC data and the shipboard display environment must incorporate both depth and time in an intuitively understandable way. Currently, we have the ability to conduct high-density hydrographic surveys capable of producing ENCs with decimeter contour intervals or depth areas. Yet, our existing systems and specifications do not provide for a full utilization of this capability. Ideally, a mariner should be able to benefit from detailed hydrographic data, coupled with both forecast and real-time water levels, and presented in a variety of perspectives. With this information mariners will be able to plan and carry out transits with the benefit of precisely determined and easily perceived underkeel, overhead, and lateral clearances. This paper describes a Hampton Roads Demonstration Project to investigate the challenges and opportunities of developing the “Electronic Chart of the Future.” In particular, a three-phase demonstration project is being planned: 1. Compile test datasets from existing and new hydrographic surveys using advanced data processing and compilation procedures developed at the University of New Hampshire’s Center for Coastal and Ocean Mapping/Joint Hydrographic Center (CCOM/JHC); 2. Investigate innovative approaches being developed at the CCOM/JHC to produce an interactive time- and tide-aware navigation display, and to evaluate such a display on commercial and/or government vessels; 3. Integrate real-time/forecast water depth information and port information services transmitted via an AIS communications broadcast

High-resolution mapping of mines and ripples at the Martha's Vineyard Coastal Observatory

Author Posting. © IEEE, 2007. This article is posted here by permission of IEEE for personal use, not for redistribution. The definitive version was published in IEEE Journal of Oceanic Engineering 32 (2007): 133-149, doi:10.1109/JOE.2007.890953.High-resolution multibeam sonar and state-of-the- art data processing and visualization techniques have been used to quantify the evolution of seafloor morphology and the degree of burial of instrumented mines and mine-shapes as part of the U.S. Office of Naval Research (ONR, Arlington, VA) mine burial experiment at the Martha's Vineyard Coastal Observatory (MVCO, Edgartown, MA). Four surveys were conducted over two years at the experiment site with a 455-kHz, Reson 8125 dynamically focused multibeam sonar. The region is characterized by shore-perpendicular alternating zones of coarse-grained sand with 5?25-cm-high, wave orbital-scale ripples, and zones of finer grained sands with smaller (2?5-cm-high) anorbital ripples and, on occasion, medium scale 10?20-cm-high, chaotic or hummocky bedforms. The boundaries between the zones appear to respond over periods of days to months to the predominant wave direction and energy. Smoothing and small shifts of the boundaries to the northeast take place during fair-weather wave conditions while erosion (scalloping of the boundary) and shifts to the north-northwest occur during storm conditions. The multibeam sonar was also able to resolve changes in the orientation and height of fields of ripples that were directly related to the differences in the prevailing wave direction and energy. The alignment of the small scale bedforms with the prevailing wave conditions appears to occur rapidly (on the order of hours or days) when the wave conditions exceed the threshold of sediment motion (most of the time for the fine sands) and particularly during moderate storm conditions. During storm events, erosional ?windows? to the coarse layer below appear in the fine-grained sands. These ?window? features are oriented parallel to the prevailing wave direction and reveal orbital-scale ripples that are oriented perpendicular to the prevailing wave direction. The resolution of the multibeam sonar combined with 3-D visualization techniques provided realistic looking images of both both instrumented and noninstrumented mines and mine-like objects (including bomb, Manta, and Rockan shapes) that were dimensionally correct and enabled unambiguous identification of the mine type. In two of the surveys (October and December 2004), the mines in the fine-grained sands scoured into local pits but were still perfectly visible and identifiable with the multibeam sonar. In the April 2004 survey, the mines were not visible and apparently were completely buried. In the coarse-grained sand zone, the mines were extremely difficult to detect after initial scour burial as the mines bury until they present the same hydrodynamic roughness as the orbital-scale bedforms and thus blend into the ambient ripple field. Given the relatively large, 3-D, spatial coverage of the multibeam sonar along with its ability to measure the depth of the seafloor and the depth and dimensions of the mine, it is possible to measure directly, the burial by depth and burial by surface area of the mines. The 3-D nature of the multibeam sonar data also allows the direct determination of the volume of material removed from a scour pit.The work of L. A. Mayer, R. Raymond, G. Glang, P. Traykovski, and A. C. Trembanis was supported by the U.S. Office of Naval Research (ONR) under the Grants N00014-01-1-0847, N00014-01-10564, and N00014-03-1-0298. The work of M. D. Richardson was supported by the U.S. Office of Naval Research (NRL) under the Core funding. The work of L. A. Mayer, R. Raymond, and G. Gland was also supported by the National Oceanic and Atmospheric Administration (NOAA) under the Grant NA17OG2285

Hydrophilic intraocular lens opacification after posterior lamellar keratoplasty - a material analysis with special reference to optical quality assessment

Background: Laboratory analysis and optical quality assessment of explanted hydrophilic intraocular lenses (IOLs) with clinically significant opacification after posterior lamellar keratoplasty (DMEK and DSAEK). Methods: Thirteen opacified IOLs after posterior lamellar keratoplasty, 8 after descemet stripping automated endothelial keratoplasty (DSAEK), 3 after descemet membrane endothelial keratoplasty (DMEK) and 2 after both DSAEK and DMEK were analysed in our laboratory. Analyses included optical bench assessment for optical quality, light microscopy, scanning electron microscopy (SEM) and energy dispersive X-Ray spectroscopy (EDS). Results: In all IOLs the opacification was caused by a thin layer of calciumphosphate that had accumulated underneath the anterior optical surface of the IOLs in the area spared by the pupil/anterior capsulorhexis. The calcifications lead to a significant deterioration of the modulation transfer function across all spatial frequencies of the affected IOLs. Conclusions: The instillation of exogenous material such as air or gas into the anterior chamber increases the risk for opacification of hydrophilic IOLs irrespective of the manufacturer or the exact composition of the hydrophilic lens material. It is recommended to avoid the use of hydrophilic acrylic IOLs in patients with endothelial dystrophy that will likely require procedures involving the intracameral instillation of air or gas, such as DMEK or DS(A)EK