Shop Talk: Annual Drosophila Research Conference, 2010

This year, the Genetics Society of America (GSA) received a record-breaking number of registrants to the conference. Despite low attendance at other scientific meetings, according to GSA Meetings Manager, Suzy Brown, this year’s conference had the ‘‘largest number of registrants than any other previous years.’’ There were 170 talks, more than 850 posters and 13 workshops; so there was a range of information that people could pick according to their interests

G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.

We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale

Violent Governance, Identity and the Production of Legitimacy: Autodefensas in Latin America

This article examines the intersections of violence, governance, identity and legitimacy in relation to autodefensas (self-defence groups) in Latin America, focusing on Mexico and Colombia. By shifting focus from the question of where legitimacy lies to how it is produced and contested by a range of groups, we challenge the often presumed link between the state and legitimacy. We develop the idea of a field of negotiation and contestation, firstly, to discuss and critique the concept of state failure as not merely a Western hegemonic claim but also a strategic means of producing legitimacy by autodefensas. Secondly, we employ and enrich the notion of violent pluralism to discuss the pervasiveness of violence and the role of neoliberalism, and to address the question of non-violent practices of governance. We argue that the idea of a field of contestation and negotiation helps to understand the complexity of relationships that encompass the production of legitimacy and identity through (non)violent governance, whereby lines between (non)state, (non)violence, and (il)legitimacy blur and transform. Yet, we do not simply dismiss (binary) distinctions as these continue to be employed by groups in their efforts to produce, justify, challenge, contest and negotiate their own and others’ legitimacy and identity

Annual Drosophila Research Conference, 2008

The meeting provides fruit fly researchers an opportunity for interaction and exchange of ideas pertaining to their research. The 49th Annual Drosophila Research Conference took place in San Diego, California, one of the best tourist destinations famous for its great weather and miles of sandy beaches. The meeting was organized by Nanci Bonini (University of Pennsylvania, Philadelphia), Susan Celnikar (Lawrence Berkeley National Laboratory, Berkeley, California), Brian Oliver (NIDDK, NIH, HHS, Bethesda, MD), and John Tamkun (University of California, Santa Cruz). This fly meeting was attended by nearly 1,500 Drosophila researchers from all over the world, who were treated to amazing presentations in 18 platform sessions encompassing 136 talks, 13 workshops, and around 1,000 posters on a broad spectrum of biomedical topics

Dissection and mounting of Drosophila pupal eye discs.

The Drosophila melanogaster eye disc is a powerful system that can be used to study many different biological processes. It contains approximately 800 separate eye units, termed ommatidia. Each ommatidium contains eight neuronal photoreceptors that develop from undifferentiated cells following the passage of the morphogenetic furrow in the third larval instar. Following the sequential differentiation of the photoreceptors, non-neuronal cells develop, including cone and pigment cells, along with mechanosensory bristle cells. Final differentiation processes, including the structured arrangement of all the ommatidial cell types, programmed cell death of undifferentiated cell types and rhodopsin expression, occurs through the pupal phase. This technique focuses on manipulating the pupal eye disc, providing insight and instruction on how to dissect the eye disc during the pupal phase, which is inherently more difficult to perform than the commonly dissected third instar eye disc. This technique also provides details on immunostaining to allow the visualization of various proteins and other cell components

Annual Drosophila Research Conference, 2011

The meeting was sponsored by the Genetics Society of America (GSA) and was organized by Daniel Barbash (Cornell University, New York), Giovanni Bosco (University of Arizona), and Leslie Griffith (Brandeis University, Massachusetts). This annual meeting brings together basic research scientists from all over the world, who study the genetic model organism, Drosophila melanogaster, the common fruit fly. This year’s meeting was a huge success with ~900 poster presentations, 156 platform sessions, 14 plenary sessions, 12 workshops, and 16 exhibits

Annual Drosophila Research Conference, 2012

The meeting was sponsored by the Genetics Society of America (GSA) and was organized by Celeste Berg (University of Washington), Steve Crews (University of North Carolina, Chapel Hill), Erika Matunis (Johns Hopkins University), and Kevin White (University of Chicago). This annual meeting brings together basic research scientists from all over the world, who study the genetic model organism, Drosophila melanogaster, the common fruit fly. This year’s meeting was a huge success with almost 1,500 preregistered attendees, ~900 poster presentations, 157 platform talks, 14 plenary lectures, 12 workshops, and 16 exhibits

Vulnerable Parkin Loss-of-Function Drosophila Dopaminergic Neurons Have Advanced Mitochondrial Aging, Mitochondrial Network Loss and Transiently Reduced Autophagosome Recruitment

Selective degeneration of substantia nigra dopaminergic (DA) neurons is a hallmark pathology of familial Parkinson’s disease (PD). While the mechanism of degeneration is elusive, abnormalities in mitochondrial function and turnover are strongly implicated. An Autosomal Recessive-Juvenile Parkinsonism (AR-JP) Drosophila melanogaster model exhibits DA neurodegeneration as well as aberrant mitochondrial dynamics and function. Disruptions in mitophagy have been observed in parkin loss-of-function models, and changes in mitochondrial respiration have been reported in patient fibroblasts. Whether loss of parkin causes selective DA neurodegeneration in vivo as a result of lost or decreased mitophagy is unknown. This study employs the use of fluorescent constructs expressed in Drosophila DA neurons that are functionally homologous to those of the mammalian substantia nigra. We provide evidence that degenerating DA neurons in parkin loss-of-function mutant flies have advanced mitochondrial aging, and that mitochondrial networks are fragmented and contain swollen organelles. We also found that mitophagy initiation is decreased in park (Drosophila parkin/PARK2 ortholog) homozygous mutants, but autophagosome formation is unaffected, and mitochondrial network volumes are decreased. As the fly ages, autophagosome recruitment becomes similar to control, while mitochondria continue to show signs of damage, and climbing deficits persist. Interestingly, aberrant mitochondrial morphology, aging and mitophagy initiation were not observed in DA neurons that do not degenerate. Our results suggest that parkin is important for mitochondrial homeostasis in vulnerable Drosophila DA neurons, and that loss of parkin-mediated mitophagy may play a role in degeneration of relevant DA neurons or motor deficits in this model