Systematic Literature Review of the Evidence for Effective National Immunisation Schedule Promotional Communications

Introduction: A systematic literature review examined the published evidence on the effectiveness of European promotional communications for national immunisation schedule (NIS) vaccinations. The review was commissioned by the European Centre for Disease Prevention and Control (ECDC) and conducted by the Institute for Social Marketing at the University of Stirling.The purpose of the review: ‘Immunisation hesitancy' has negatively impacted population uptake of routine immunisation. A substantial body of evaluated communication activity promoting nationally indicated routine immunisation has been published. This systematic review of the evidence aims to: collate and map the types of promotional communication that have been used; assess the quality of the evaluative research reporting on these promotional communications; and assess the applicability of this evidence to immunisation policy, strategy and practice priorities. The analysis and findings are intended to provide a current status report on the evidence, and evidence gaps for good practice in national immunisation promotional communications, thus supporting countries in their communication activities for the prevention and control of communicable diseases.Objectives of the review: The review aimed to answer the following research questions: Which audiences have been targeted by NIS promotional communications? Which communication methods and approaches have been used to promote or reinforce NIS vaccination uptake? What theoretical underpinnings are used to inform communication methods and approaches? Which settings and communication channels have been used to promote or reinforce NIS vaccination uptake? What is the evidence for effectiveness of communication initiatives in changing or reinforcing knowledge, attitudes or behaviour towards NIS? What is the evidence for impact of NIS communication initiatives to control communicable disease? What impact have campaign communications promoting NIS had on public acceptance and vaccine uptake rates

Betaglycan Is Required for the Establishment of Nephron Endowment in the Mouse

Betaglycan is an accessory receptor for the transforming growth factor-β (TGFβ) superfamily, many members of which play key roles in kidney development. The purpose of this study was to define the role of this co-receptor on fetal murine kidney development. Stereological examination of embryonic and adult betaglycan heterozygous kidneys revealed augmented nephron number relative to littermate controls. Fetal heterozygous kidneys exhibited accelerated ureteric branching, which correlated with augmented nephron development at embryonic day (e) 15.5. In contrast, betaglycan null kidneys exhibited renal hypoplasia from e13.5 and reduced nephron number at e15.5. Quantitative real-time PCR analysis of e11.5–e14.5 kidneys demonstrated that heterozygous kidneys exhibited a transient decrease in Bmp4 expression at e11.5 and a subsequent cascade of changes in the gene regulatory network that governs metanephric development, including significant increases in Pax2, Eya1, Gdnf, Ret, Wnt4, and Wt1 expression. Conversely, gene expression in null kidneys was normal until e13.5, when significant reductions were detected in the expression of Bmp4 as well as other key metanephric regulatory genes. Tgfb1 and Tgfb2 mRNA expression was down-regulated in both nulls and heterozygotes at e13.5 and e14.5. The opposing morphological and molecular phenotypes in betaglycan heterozygote and null mutants demonstrate that the levels of betaglycan must be tightly regulated for optimal kidney development

Multi-physics ensemble modelling of Arctic tundra snowpack properties

Sophisticated snowpack models such as Crocus and SNOWPACK struggle to properly simulate profiles of density and specific surface area (SSA) within Arctic snowpacks due to an underestimation of wind-induced compaction, misrepresentation of basal vegetation influencing compaction and metamorphism, and omission of water vapour flux transport. To improve the simulation of profiles of density and SSA, parameterisations of snow physical processes that consider the effect of high wind speeds, the presence of basal vegetation and alternate thermal conductivity formulations were implemented into an ensemble version of the Soil, Vegetation and Snow version 2 (SVS2-Crocus) land surface model, creating Arctic SVS2-Crocus. The ensemble versions of default and Arctic SVS2-Crocus were driven with in-situ meteorological data and evaluated using measurements of snowpack properties (SWE, depth, density and SSA) at Trail Valley Creek (TVC), Northwest Territories, Canada over 32-years (1991–2023). Results show that both default and Arctic SVS2-Crocus can simulate the correct magnitude of SWE (RMSE for both ensembles: 55 kg m-2) and snow depth (default RMSE: 0.22 m; Arctic RMSE: 0.18 m) at TVC in comparison to measurements. Wind-induced compaction within Arctic SVS2-Crocus effectively compacts the surface layers of the snowpack, increasing the density, and reducing the RMSE by 41 % (176 kg m-3 to 103 kg m-3). Parameterisations of basal vegetation are less effective in reducing compaction of basal snow layers (default RMSE: 67 kg m-3; Arctic RMSE: 65 kg m-3), reaffirming the need to consider water vapour flux transport for simulation of low-density basal layers. The top 100 ensemble members of Arctic SVS2-Crocus produced lower continuous ranked probability scores (CRPS) than default SVS2-Crocus when simulating snow density profiles. The top performing members of the Arctic SVS2-Crocus ensemble featured modifications that raise wind speeds to increase compaction in snow surface layers and prevent snowdrift and increase viscosity in basal layers. Selecting these process representations in Arctic SVS2-Crocus will improve simulation of snow density profiles, which is crucial for many applications

Social technologies for online learning: theoretical and contextual issues

Three exemplars are presented of social technologies deployed in educational contexts: wikis; a photo-sharing environment; and a social bookmarking tool. Students were found to engage with the technologies selectively, sometimes rejecting them, in the light of their prior conceptions of education. Some students (a minority in all the studies) were unsympathetic to the educational philosophy underpinning the technology’s adoption. The paper demonstrates, through an examination of in-context use, the importance of socio-cultural factors in relation to education, and the non-deterministic nature of educational technology. The academic study of technology has increasingly called into question the deterministic views which are so pervasive in popular discourse and among policy makers. Instead, socio-cultural factors play a crucial role in shaping and defining technology and educational technology is no exception, as the examples in the paper show. The paper concludes by drawing out some implications of the examples for the use of social technologies in education

Prospective case study of critical decision making for horses referred for treatment of colic

BackgroundThere is limited evidence on factors affecting critical decision making for horses with colic. This study's aim was to describe the assessment and decision making involved in horses referred for management of colic.MethodsAn in-depth case analysis was used to document case presentation, decision making and outcomes for horses referred for colic to two UK equine veterinary practices over a 12-month period. The data recorded included previous history, presenting signs, response to treatment, case outcome and factors affecting decisions for further treatment or euthanasia.ResultsData were available for 60 cases: 55 were hospitalised for medical or surgical treatment and five horses were euthanased following initial assessment. The main factors affecting treatment decisions were severity of clinical signs (80%, 47/59), financial concerns (10%, 6/59) and ongoing health issues or previous history of colic (5%, 3/59). Factors associated with euthanasia decisions were postoperative complications (5/18), poor prognosis (4/18), deteriorating clinical signs (3/18) and financial concerns (3/18).LimitationThe limited study population may affect the extent to which the findings can be generalised.ConclusionThe severity of clinical signs was the key factor in treatment decision making. The main factors affecting euthanasia decisions were clinical deterioration, poor prognosis and financial constraints. These findings should be incorporated into clinical case recording to capture the multifactorial nature of decision making

A symmetric direct ammonia fuel cell using ternary NiCuFe alloy embedded in a carbon network as electrodes

Recently, due to the high energy density of ammonia, low source-to-tank energy cost and carbon free fuel, low-temperature direct ammonia fuel cells (DAFCs) have attracted extensive attention and play an important role in ammonia economy. In DAFCs, Pt-based materials have been the most studied electrocatalysts for the anodic ammonia oxidation reaction (AOR) and cathodic oxygen reduction reaction (ORR) over the past decade. However, the high cost of precious metal has markedly inhibited the large-scale application of DAFCs. Herein, a bifunctional material of ternary Ni4Cu5Fex (x = 0, 0.5, 1, 3, 5) alloy embedded in a carbon network was prepared by a simple solvothermal approach, and showed superior activity and durability towards both AOR and ORR. The excellent bifunctional catalytic activity of the Ni4Cu5Fe1/C sample was analyzed by experimental and calculational methods. Utilizing the bifunctional activities of this alloy, a symmetric DAFC was assembled with Ni4Cu5Fe1/C as both the anode and cathode, with a commercial anion exchange membrane (AEM) as electrolyte. The symmetric DAFC-Ni4Cu5Fe1/C showed a maximum current density of 67 mA cm−2 at 80 °C. To the best of our knowledge, this is the first report on a symmetric DAFC. This work not only reports a new AOR/ORR bifunctional catalyst, but also moves towards the development of low-cost DAFCs with simple structure

The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing

Peer reviewedPublisher PD

Augmenting forearm crutches with wireless sensors for lower limb rehabilitation

Forearm crutches are frequently used in the rehabilitation of an injury to the lower limb. The recovery rate is improved if the patient correctly applies a certain fraction of their body weight (specified by a clinician) through the axis of the crutch, referred to as partial weight bearing (PWB). Incorrect weight bearing has been shown to result in an extended recovery period or even cause further damage to the limb. There is currently no minimally invasive tool for long-term monitoring of a patient's PWB in a home environment. This paper describes the research and development of an instrumented forearm crutch that has been developed to wirelessly and autonomously monitor a patient's weight bearing over the full period of their recovery, including its potential use in a home environment. A pair of standard forearm crutches are augmented with low-cost off-the-shelf wireless sensor nodes and electronic components to provide indicative measurements of the applied weight, crutch tilt and hand position on the grip. Data are wirelessly transmitted between crutches and to a remote computer (where they are processed and visualized in LabVIEW), and the patient receives biofeedback by means of an audible signal when they put too much or too little weight through the crutch. The initial results obtained highlight the capability of the instrumented crutch to support physiotherapists and patients in monitoring usage

An efficient symmetric electrolyzer based on bifunctional perovskite catalyst for ammonia electrolysis

Ammonia is a natural pollutant in wastewater and removal technique such as ammonia electro‐oxidation is of paramount importance. The development of highly efficient and low‐costing electrocatalysts for the ammonia oxidation reaction (AOR) and hydrogen evolution reaction (HER) associated with ammonia removal is subsequently crucial. In this study, for the first time, the authors demonstrate that a perovskite oxide LaNi0.5Cu0.5O3‐δ after being annealed in Ar (LNCO55‐Ar), is an excellent non‐noble bifunctional catalyst towards both AOR and HER, making it suitable as a symmetric ammonia electrolyser (SAE) in alkaline medium. In contrast, the LNCO55 sample fired in air (LNCO55‐Air) is inactive towards AOR and shows very poor HER activity. Through combined experimental results and theoretical calculations, it is found that the superior AOR and HER activities are attributed to the increased active sites, the introduction of oxygen vacancies, the synergistic effect of B‐site cations and the different active sites in LNCO55‐Ar. At 1.23 V, the assembled SAE demonstrates ≈100% removal efficiency in 2210 ppm ammonia solution and >70% in real landfill leachate. This work opens the door for developments towards bifunctional catalysts, and also takes a profound step towards the development of low‐costing and simple device configuration for ammonia electrolysers

Propionic acid promotes the virulent phenotype of Crohn's disease-associated adherent-invasive Escherichia coli

Propionic acid (PA) is a bacterium-derived intestinal antimicrobial and immune modulator used widely in food production and agriculture. Passage of Crohn’s disease-associated adherent-invasive Escherichia coli (AIEC) through a murine model, in which intestinal PA levels are increased to mimic the human intestine, leads to the recovery of AIEC with significantly increased virulence. Similar phenotypic changes are observed outside the murine model when AIEC is grown in culture with PA as the sole carbon source; such PA exposure also results in AIEC that persists at 20-fold higher levels in vivo. RNA sequencing identifies an upregulation of genes involved in biofilm formation, stress response, metabolism, membrane integrity, and alternative carbon source utilization. PA exposure also increases virulence in a number of E. coli isolates from Crohn’s disease patients. Removal of PA is sufficient to reverse these phenotypic changes. Our data indicate that exposure to PA results in AIEC resistance and increased virulence in its presence