-mediated recombination of the allele in spermatogenic cells of double transgenic mouse

() Histograms of flow cytometry of testicular cell suspension obtained from a 60-day-old double transgenic mouse showed a distribution of 65%, 9%, and 17% for 1N, 2N, and 4N cells, respectively. PCR of genomic DNA from 1N and 4N cells showed excision of the allele but not from 2N cells. () Histogram showed enrichment of 2N cells by Percoll gradients as described in Methods. PCR analysis of these enriched 2N cells demonstrated excision of the allele. Primers b and c identify wild type (390 bp) and floxed allele (450 bp) whereas primers a and c identify the excised allele (500 bp). TC, testicular cells; M, DNA marker.<p><b>Copyright information:</b></p><p>Taken from "Promiscuous recombination of alleles during gametogenesis in cornea driver mice"</p><p></p><p>Molecular Vision 2008;14():562-571.</p><p>Published online 20 Mar 2008</p><p>PMCID:PMC2274927.</p><p></p

Expression of the gene in double transgenic mice during spermatogenesis

Whole mount X-gal staining of testes collected from 20-(), 30-(), and 60 day-old () bitransgenic mice showed positive reaction in primary and secondary spermatocytes and spermatids. No β-gal activity was found in adult single transgenic testes and 10-day-old testes of double mice (data not shown). Leydig cells (arrowheads) have endogenous β-gal activity. Testicular cell suspensions prepared from a mouse were subjected to May–Grünwald-Giemsa stain after X-gal staining. Positive X-gal staining (arrow) was observed in the primary spermatocytes (), primary spermatocytes in meiotic division (), spermatids (), and round spermatids ().<p><b>Copyright information:</b></p><p>Taken from "Promiscuous recombination of alleles during gametogenesis in cornea driver mice"</p><p></p><p>Molecular Vision 2008;14():562-571.</p><p>Published online 20 Mar 2008</p><p>PMCID:PMC2274927.</p><p></p