Whole mount X-gal staining of testes collected from 20-(), 30-(), and 60 day-old () bitransgenic mice showed positive reaction in primary and secondary spermatocytes and spermatids. No β-gal activity was found in adult single transgenic testes and 10-day-old testes of double mice (data not shown). Leydig cells (arrowheads) have endogenous β-gal activity. Testicular cell suspensions prepared from a mouse were subjected to May–Grünwald-Giemsa stain after X-gal staining. Positive X-gal staining (arrow) was observed in the primary spermatocytes (), primary spermatocytes in meiotic division (), spermatids (), and round spermatids ().<p><b>Copyright information:</b></p><p>Taken from "Promiscuous recombination of alleles during gametogenesis in cornea driver mice"</p><p></p><p>Molecular Vision 2008;14():562-571.</p><p>Published online 20 Mar 2008</p><p>PMCID:PMC2274927.</p><p></p