Quantifying the radiation belt seed population in the 17 March 2013 electron acceleration event

Abstract We present phase space density (PSD) observations using data from the Magnetic Electron Ion Spectrometer instrument on the Van Allen Probes for the 17 March 2013 electron acceleration event. We confirm previous results and quantify how PSD gradients depend on the first adiabatic invariant. We find a systematic difference between the lower-energy electrons (1-MeV with a source region within the radiation belts. Our observations show that the source process begins with enhancements to the 10s-100s-keV energy seed population, followed by enhancements to the \u3e1-MeV population and eventually leading to enhancements in the multi-MeV electron population these observations provide the clearest evidence to date of the timing and nature of the radial transport of a 100s keV electron seed population into the heart of the outer belt and subsequent local acceleration of those electrons to higher radiation belt energies. Key Points Quantification of phase space density gradients inside geostationary orbit Clear differences between the source of low energy and relativistic electrons Clear observations of how the acceleration process evolves in energy

Rapid estimation of sugar release from winter wheat straw during bioethanol production using FTIR-photoacoustic spectroscopy

BACKGROUND: Complexity and high cost are the main limitations for high-throughput screening methods for the estimation of the sugar release from plant materials during bioethanol production. In addition, it is important that we improve our understanding of the mechanisms by which different chemical components are affecting the degradability of plant material. In this study, Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was combined with advanced chemometrics to develop calibration models predicting the amount of sugars released after pretreatment and enzymatic hydrolysis of wheat straw during bioethanol production, and the spectra were analysed to identify components associated with recalcitrance. RESULTS: A total of 1122 wheat straw samples from nine different locations in Denmark and one location in the United Kingdom, spanning a large variation in genetic material and environmental conditions during growth, were analysed. The FTIR-PAS spectra of non-pretreated wheat straw were correlated with the measured sugar release, determined by a high-throughput pretreatment and enzymatic hydrolysis (HTPH) assay. A partial least square regression (PLSR) calibration model predicting the glucose and xylose release was developed. The interpretation of the regression coefficients revealed a positive correlation between the released glucose and xylose with easily hydrolysable compounds, such as amorphous cellulose and hemicellulose. Additionally, a negative correlation with crystalline cellulose and lignin, which inhibits cellulose and hemicellulose hydrolysis, was observed. CONCLUSIONS: FTIR-PAS was used as a reliable method for the rapid estimation of sugar release during bioethanol production. The spectra revealed that lignin inhibited the hydrolysis of polysaccharides into monomers, while the crystallinity of cellulose retarded its hydrolysis into glucose. Amorphous cellulose and xylans were found to contribute significantly to the released amounts of glucose and xylose, respectively. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-015-0267-2) contains supplementary material, which is available to authorized users

On the cause and extent of outer radiation belt losses during the 30 September 2012 dropout event

Abstract On 30 September 2012, a flux dropout occurred throughout Earth\u27s outer electron radiation belt during the main phase of a strong geomagnetic storm. Using eight spacecraft from NASA\u27s Time History of Events and Macroscale Interactions during Substorms (THEMIS) and Van Allen Probes missions and NOAA\u27s Geostationary Operational Environmental Satellites constellation, we examined the full extent and timescales of the dropout based on particle energy, equatorial pitch angle, radial distance, and species. We calculated phase space densities of relativistic electrons, in adiabatic invariant coordinates, which revealed that loss processes during the dropout were \u3e 90% effective throughout the majority of the outer belt and the plasmapause played a key role in limiting the spatial extent of the dropout. THEMIS and the Van Allen Probes observed telltale signatures of loss due to magnetopause shadowing and subsequent outward radial transport, including similar loss of energetic ring current ions. However, Van Allen Probes observations suggest that another loss process played a role for multi-MeV electrons at lower L shells (L\u3c ∼4). Key Points Dropout events can encompass the entire outer radiation belt Dropouts can result in \u3e90% losses and be a hard reset on the system Loss at L \u3e ∼4 is dominated by MP shadowing and outward transport

Competing source and loss mechanisms due to wave-particle interactions in Earth’s outer radiation belt during the 30 September to 3 October 2012 geomagnetic storm

Abstract Drastic variations of Earth’s outer radiation belt electrons ultimately result from various competing source, loss, and transport processes, to which wave-particle interactions are critically important. Using 15 spacecraft including NASA’s Van Allen Probes, THEMIS, and SAMPEX missions and NOAA’s GOES and POES constellations, we investigated the evolution of the outer belt during the strong geomagnetic storm of 30 September to 3 October 2012. This storm’s main phase dropout exhibited enhanced losses to the atmosphere at L*\u3c 4, where the phase space density (PSD) of multi-MeV electrons dropped by over an order of magnitude in1 MeV electrons and energetic protons, SAMPEX \u3e1 MeV electrons, and ground observations of band-limited Pc1-2 wave activity, we show that this sudden loss was consistent with pitch angle scattering by electromagnetic ion cyclotron waves in the dusk magnetic local time sector at 3\u3c L*\u3c 4. At 4\u3c L*\u3c 5, local acceleration was also active during the main and early recovery phases, when growing peaks in electron PSD were observed by both Van Allen Probes and THEMIS. This acceleration corresponded to the period when IMF Bz was southward, the AE index was \u3e300 nT, and energetic electron injections and whistler-mode chorus waves were observed throughout the inner magnetosphere for \u3e12 h. After this period, Bz turned northward, and injections, chorus activity, and enhancements in PSD ceased. Overall, the outer belt was depleted by this storm. From the unprecedented level of observations available, we show direct evidence of the competitive nature of different wave-particle interactions controlling relativistic electron fluxes in the outer radiation belt

Identification and Overexpression of a Knotted1-Like Transcription Factor in Switchgrass (Panicum virgatum L.) for Lignocellulosic Feedstock Improvement

High biomass production and wide adaptation has made switchgrass (Panicum virgatum L.) an important candidate lignocellulosic bioenergy crop. One major limitation of this and other lignocellulosic feedstocks is the recalcitrance of complex carbohydrates to hydrolysis for conversion to biofuels. Lignin is the major contributor to recalcitrance as it limits the accessibility of cell wall carbohydrates to enzymatic breakdown into fermentable sugars. Therefore, genetic manipulation of the lignin biosynthesis pathway is one strategy to reduce recalcitrance. Here, we identified a switchgrass Knotted1 transcription factor, PvKN1, with the aim of genetically engineering switchgrass for reduced biomass recalcitrance for biofuel production. Gene expression of the endogenousPvKN1 gene was observed to be highest in young inflorescences and stems. Ectopic overexpression of PvKN1 in switchgrass altered growth, especially in early developmental stages. Transgenic lines had reduced expression of most lignin biosynthetic genes accompanied by a reduction in lignin content suggesting the involvement of PvKN1 in the broad regulation of the lignin biosynthesis pathway. Moreover, the reduced expression of the Gibberellin 20-oxidase (GA20ox) gene in tandem with the increased expression of Gibberellin 2-oxidase (GA2ox) genes in transgenic PvKN1 lines suggest that PvKN1 may exert regulatory effects via modulation of GA signaling. Furthermore, overexpression of PvKN1 altered the expression of cellulose and hemicellulose biosynthetic genes and increased sugar release efficiency in transgenic lines. Our results demonstrated that switchgrass PvKN1 is a putative ortholog of maize KN1 that is linked to plant lignification and cell wall and development traits as a major regulatory gene. Therefore, targeted overexpression of PvKN1 in bioenergy feedstocks may provide one feasible strategy for reducing biomass recalcitrance and simultaneously improving plant growth characteristics

Design of Z-Pinch and Dense Plasma Focus Powered Vehicles

Z-pinch and Dense Plasma Focus (DPF) are two promising techniques for bringing fusion power to the field of in-space propulsion. A design team comprising of engineers and scientists from UAHuntsville, NASA's George C. Marshall Space Flight Center and the University of Wisconsin developed concept vehicles for a crewed round trip mission to Mars and an interstellar precursor mission. Outlined in this paper are vehicle concepts, complete with conceptual analysis of the mission profile, operations, structural and thermal analysis and power/avionics design. Additionally engineering design of the thruster itself is included. The design efforts adds greatly to the fidelity of estimates for power density (alpha) and overall performance for these thruster concept

Transgenic miR156 Switchgrass in the Field: Growth, Recalcitrance and Rust Susceptibility

Sustainable utilization of lignocellulosic perennial grass feedstocks will be enabled by high biomass production and optimized cell wall chemistry for efficient conversion into biofuels. MicroRNAs are regulatory elements that modulate the expression of genes involved in various biological functions in plants, including growth and development. In greenhouse studies, overexpressing a microRNA (miR156) gene in switchgrass had dramatic effects on plant architecture and flowering, which appeared to be driven by transgene expression levels. Highexpressing lines were extremely dwarfed, whereas low and moderate-expressing lines had higher biomass yields, improved sugar release and delayed flowering. Four lines with moderate or low miR156 overexpression from the prior greenhouse study were selected for a field experiment to assess the relationship between miR156 expression and biomass production over three years. We also analysed important bioenergy feedstock traits such as flowering, disease resistance, cell wall chemistry and biofuel production. Phenotypes of the transgenic lines were inconsistent between the greenhouse and the field as well as among different field growing seasons. One low expressing transgenic line consistently produced more biomass (25%–56%) than the control across all three seasons, which translated to the production of 30% more biofuel per plant during the final season. The other three transgenic lines produced less biomass than the control by the final season, and the two lines with moderate expression levels also exhibited altered disease susceptibilities. Results of this study emphasize the importance of performing multiyear field studies for plants with altered regulatory transgenes that target plant growth and development

The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release

Background Genetically engineered biofuel crops, such as switchgrass (Panicum virgatum L.), that produce their own cell wall-digesting cellulase enzymes would reduce costs of cellulosic biofuel production. To date, non-bioenergy plant models have been used in nearly all studies assessing the synthesis and activity of plant-produced fungal and bacterial cellulases. One potential source for cellulolytic enzyme genes is herbivorous insects adapted to digest plant cell walls. Here we examine the potential of transgenic switchgrass-produced TcEG1 cellulase from Tribolium castaneum (red flour beetle). This enzyme, when overproduced in Escherichia coliand Saccharomyces cerevisiae, efficiently digests cellulose at optima of 50 °C and pH 12.0. Results TcEG1 that was produced in green transgenic switchgrass tissue had a range of endoglucanase activity of 0.16–0.05 units (µM glucose release/min/mg) at 50 °C and pH 12.0. TcEG1 activity from air-dried leaves was unchanged from that from green tissue, but when tissue was dried in a desiccant oven (46 °C), specific enzyme activity decreased by 60%. When transgenic biomass was “dropped-in” into an alkaline buffer (pH 12.0) and allowed to incubate at 50 °C, cellobiose release was increased up to 77% over non-transgenic biomass. Saccharification was increased in one transgenic event by 28%, which had a concurrent decrease in lignin content of 9%. Histological analysis revealed an increase in cell wall thickness with no change to cell area or perimeter. Transgenic plants produced more, albeit narrower, tillers with equivalent dry biomass as the control. Conclusions This work describes the first study in which an insect cellulase has been produced in transgenic plants; in this case, the dedicated bioenergy crop switchgrass. Switchgrass overexpressing the TcEG1 gene appeared to be morphologically similar to its non-transgenic control and produced equivalent dry biomass. Therefore, we propose TcEG1 transgenics could be bred with other transgenic germplasm (e.g., low-lignin lines) to yield new switchgrass with synergistically reduced recalcitrance to biofuel production. In addition, transgenes for other cell wall degrading enzymes may be stacked with TcEG1 in switchgrass to yield complementary cell wall digestion features and complete auto-hydrolysis