27 research outputs found
Peran Gaya Kepemimpinan Transformasional Memoderasi Pengaruh Motivasi Intrinsik dan Kecerdasan Emosional terhadap Kinerja Guru (Studi Kasus pada SMA Negeri di Kecamatan Pati Kabupaten Pati)
This research is intended to examine the influence of motivation intrinsic and emotional intelligence to the state senior high school teachers\u27 work performance with the moderation of transformational leadership style. The specific purpose of this research is to examine the role of transformational leadership style moderates the influence of intrinsic motivation and emotional intelligence to the teachers\u27 work performance. The USAge of this research is to explain and expand the previous research about the role of transformational leadership style moderates the influence of intrinsic motivation and emotional intelligence to the teachers\u27 work performance. This research used the population of 116 teachers of state senior high school in Pati District, Pati Regency. The technique of sample collection used in this research is non-probability sampling with the purposive method. The analysis technique used in this research is regression model moderate quasi. Based on the research result can be conduded that: intrinsic motivation influences teachers\u27 work performance, emotional intelligence influences the teachers\u27 work performance, transformational leadership style do not moderate the influence of intrinsic motivation to teachers\u27 work performance, , transformational leadership style strengthen the influence of emotional intelligence to the teachers\u27 workperformance
Digital Gene Expression Analysis Based on <i>De Novo</i> Transcriptome Assembly Reveals New Genes Associated with Floral Organ Differentiation of the Orchid Plant <i>Cymbidium ensifolium</i>
<div><p><i>Cymbidium ensifolium</i> belongs to the genus <i>Cymbidium</i> of the orchid family. Owing to its spectacular flower morphology, <i>C</i>. <i>ensifolium</i> has considerable ecological and cultural value. However, limited genetic data is available for this non-model plant, and the molecular mechanism underlying floral organ identity is still poorly understood. In this study, we characterize the floral transcriptome of <i>C</i>. <i>ensifolium</i> and present, for the first time, extensive sequence and transcript abundance data of individual floral organs. After sequencing, over 10 Gb clean sequence data were generated and assembled into 111,892 unigenes with an average length of 932.03 base pairs, including 1,227 clusters and 110,665 singletons. Assembled sequences were annotated with gene descriptions, gene ontology, clusters of orthologous group terms, the Kyoto Encyclopedia of Genes and Genomes, and the plant transcription factor database. From these annotations, 131 flowering-associated unigenes, 61 <i>CONSTANS-LIKE</i> (<i>COL</i>) unigenes and 90 floral homeotic genes were identified. In addition, four digital gene expression libraries were constructed for the sepal, petal, labellum and gynostemium, and 1,058 genes corresponding to individual floral organ development were identified. Among them, eight MADS-box genes were further investigated by full-length cDNA sequence analysis and expression validation, which revealed two <i>APETALA1/AGL9</i>-like MADS-box genes preferentially expressed in the sepal and petal, two <i>AGAMOUS</i>-like genes particularly restricted to the gynostemium, and four <i>DEF</i>-like genes distinctively expressed in different floral organs. The spatial expression of these genes varied distinctly in different floral mutant corresponding to different floral morphogenesis, which validated the specialized roles of them in floral patterning and further supported the effectiveness of our <i>in silico</i> analysis. This dataset generated in our study provides new insights into the molecular mechanisms underlying floral patterning of <i>Cymbidium</i> and supports a valuable resource for molecular breeding of the orchid plant.</p></div
Transcripts differentially expressed between different floral organs.
<p>Up- and down-regulated transcripts were quantified. The results of six comparisons between each two samples are shown. 1, sepal; 2, petal; 3, Labellum; 4, gynostemium.</p
The length distribution of assembled unigenes.
<p>The x-axis represents the sequence length in base pairs. The y-axis represents the unigenes number.</p
The quantitative RT-PCR analysis of gene expression in sepals, petals, labellums and gynostemiums.
<p>The y-axis indicates fold change in expression among the samples. The Lg (Relative Quantitation) of the genes in the sepals was calibrated as zero. Error bars indicate the standard deviation of the mean (SD) (n = 3). Three replicates were analyzed, with similar results. a, b, and c, d, one way ANOVA with Bonferroni multiple comparison test significant at P<0.05 between two of the individual floral organs sepal, petal, Labellum, gynostemium.</p
Sequence comparison of the DEF-like genes of <i>C</i>. <i>ensifolium</i> and <i>P</i>. <i>equestris</i>.
<p>Amino acid sequences were aligned by the ClustalW 2.0. The MADS-, I-, K-, and C-domains are indicated on top of the column.</p
Predicted transcription factors of <i>Cymbidium ensifolium</i>.
<p>Predicted transcription factors of <i>Cymbidium ensifolium</i>.</p
Tissue-specific genes revealed from DGE expression of various flower organs.
<p>Tissue-specific genes revealed from DGE expression of various flower organs.</p
Transcription factors differentially expressed among different floral organs.
<p>Up- and down-regulated transcripts were quantified. The results of six comparisons between each two samples are shown. 1, sepal; 2, petal; 3, Labellum; 4, gynostemium.</p
Transcription factors correlated with the development of the gynostemium.
<p>Up-(red) and down-regulated (green) in the gynostemium as opposed to other DGEs were quantified and annotated with the plant transcription factor database.</p