30 research outputs found
Characterization of a circulating PRRSV strain by means of random PCR cloning and full genome sequencing
Get PDFRS is a pig disease of major economic importance that causes respiratory and reproductive problems in pigs. Over the last years it has become clear that PRRSV heterogeneity is increasing. Consequently, this has a potential impact on diagnosis and strategies to counter this disease. The use of sequence-independent PCR techniques for the detection and characterization of PRRSV could be useful to bypass problems associated with the heterogeneity of this virus. A random PCR cloning approach was tested for the characterization of PRRSV strain 07V063 of unknown genetic background that circulated on a Belgian farm. By using this approach, 7305 bp of sequence data were obtained, distributed randomly across the genome. Using RT-PCR with strain-specific primers, the full length sequence (15014 nt) was obtained. Phylogenetic relationships using ORF5 and ORF1a (NSP2) sequences showed that 07V063 was classified in type 1 subtype 1 and that 07V063 was genetically different from prototype Lelystad Virus (LV). 07V063 showed 87-93% aa identity with LV ORFs coding for structural proteins. Most variation (compared to LV) was noticed in Nsp2 (81% identity) with a deletion of 28 aa. This deletion was different from other known deletions in this ORF. In conclusion, it is shown that this random PCR cloning approach can be used for the characterization of new PRRSV strains of unknown genetic background
Pénétration - perméation - fermentation: Réflexions sur l'être de la liturgie et ses modes de mémoire
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A Paradigm of Permeability: Franz von Baader on Love
No full text© 2016 International Journal of Philosophy and Theology. Bavarian intellectual Franz Xaver von Baader (1765–1841) counts among the most prominent representatives of German Romanticism, although his name and fame have almost been forgotten. Baader was a key figure among the Romantic scene and an ardent defender of Catholicism in the aftermath of Enlightenment criticism on the Christian faith and tradition. Most interesting is that he did not construe his apologia on the basis of rational considerations only, as a counterattack as it were, but that he took a point of departure in a peculiar understanding of love. According to Baader, love is a concept and a reality which allows one to imagine the relation between creature and Creator and which intriguingly combines the distinctively human with the uniquely divine. In the present paper, I am continuing in the line of Ramón Betanzos’ outstanding study on Baader’s idea of love and argue that his intuitions reveal a solid synthesis of theological and philosophical insights which today is definitely worth reconsidering. In order to do that in an orderly fashion, I primordially focus on his concept of permeability and explore its metaphysical, epistemological and anthropological potential.status: publishe
Lichaam van Christus worden: Voor een vernieuwde fascinatie voor liturgie-intuĂŻties
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Paschal Joy Continued: Exploring Leo the Great's Theology of Christ's Ascension into Heaven
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