Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method

The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue culture samples of H. littoralis.The separationwas achieved using a reversed-phase column. The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the samples. The highest lycorine content was found in the bulb extract (2.54 ± 0.0

Potential antioxidant activities of methanolic extracts of Spider lily (Hymenocallis littoralis)

Abstract Hymenocallis littoralis has allelochemical importance such as defensive compounds, insect repellents, attractants, and their role in ecological balance. Antioxidant activities of Hymenocallis littoralis bulb, anther, flower, stem, leaves and root methanolic extracts were evaluated using Ferric Reducing Antioxdiant Power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic and flavonoid assays. Dried parts were ground and extracted using sonication method. The extracts were then tested for their antioxidant activity by FRAP, DPPH, total phenolic and flavonoid assays. The flower and anther methanolic extracts shows ferric reducing antioxidant power (FRAP) at 555.12 ± 1.67 and 568.09 ± 0.42 µmol g -1 Fe 2+ . High activity for DPPH free radical molecules was observed in flower (1.29 mg mL -1 ), stem (1.33 mg mL -1 ), and anther (0.31 mg mL -1 ). The phenolic content of Hymenocallis littoralis extracts are in ascending order as per root < leaves < stem < bulb < flower < anther. The flavanoid content of Hymenocallis litoralis plant extracts in ascending order are bulb < root < leaves < flower < stem < anther. Elucidation and isolation of the active compounds from this plant can help to shed more information of the compound of interest. This finding shows that the Hymenocallis littoralis bulb, root and anther extracts possess a good antioxidant activity which can demonstrate a better cytotoxicity activity

In Vitro Antioxidant and Xanthine Oxidase Inhibitory Activities of Methanolic Swietenia mahagoni Seed Extracts

This study examines the in vitro antioxidant activities of the methanol extract of Swietenia mahagoni seeds (SMCM seed extract). The extract was screened for possible antioxidant activities by free radical scavenging activity (DPPH), xanthine oxidase inhibition (XOI), hydrogen peroxide scavenging activity (HPSA) and ferric-reducing antioxidant power (FRAP) assays. The total phenolic and flavonoid contents were also determined. The extract exhibits antioxidant activity of 23.29% with an IC50 value of 2.3 mg/mL in the DPPH radical scavenging method, 47.2% in the XOI assay, 49.5% by the HPSA method, and 0.728 mmol/Fe(II)g in the FRAP method at the concentration tested. The amount of total phenolics and flavonoid contents was 70.83 mg gallic acid equivalent (GAE) and 2.5 ± 0.15 mg of catechin equivalent per gram of dry extract, respectively. High Performance Thin Layer Chromatography (HPTLC) screening indicates the presence of phenolic compounds in the SMCM seed extract. The results indicate that the extract has both high free radical scavenging and xanthine oxidase inhibition activity. The antioxidant activity of SMCM seed extract is comparable with that of other Malaysian tropical fruits and herbal plants