Kinetic Characterization and Partial Purification of Peroxidase in Eucalyptus F1 Hybrids

A differential pattern of peroxidase (E.C. 1.1.11.7) has been observed in reciprocal F1 hybrids of Eucalyptus (FRI-4 and FRI-5) produced using parental combination of E. tereticornis and E. camaldulensis. The hybrids were characterized for the qualitative, kinetic parameters (total and specific activity, units, Km and Vmax) and partial purification of peroxidase. The qualitative study showed the presence of high molecular weight peroxidase isoforms i.e. 260 KD, 250 KD and 110KD bands. A strong maternal inheritance was observed for 260 KD loci in FRI-5. Kinetic characterization of peroxidase revealed that F1 genotypes had efficient peroxidase system over their parental combination resulting in 8 -10 times more heterotic vigour. The FRI-5 genotypes were found to be having efficient peroxidase system with lowest Km values (0.12mM-0.17mM) and higher Vmax values ranging from 0.07-0.14mM min-1mg prot-1. The study revealed the role of maternal inheritance and was found that better genotypes are produced when superior parents are used as seed parent. The results suggest the efficient use of peroxidase marker for the identification of the better genotypes in forest tree species. &nbsp

Gabapentin a pre-emptive analgesic in post-operative pain: a randomised double blind placebo controlled study

Background: Conventional analgesics, used in peri-operative period cause numerous adverse effects and are not free from interactions with co-administered drugs. Gabapentin has been shown to be effective in various types of neuropathic pain. The primary aim of this study was to evaluate gabapentin as a post-operative analgesic. The study also evaluates the analgesic requirement and safety of gabapentin in post-operative period.Methods: Forty patients undergoing elective laparoscopic cholecystectomy were randomized to receive gabapentin or a matching placebo. The patients of group I received gabapentin 600mg orally 2 hrs before surgery and 12hrs after the first dose. The patients in group II received a matching placebo. Patients in both groups received diclofenac sodium 75mg i.m b.i.d for pain. Additional doses were given on demand and recorded.Results: The present study found that gabapentin significantly reduced pain score and analgesic consumption as compared to a placebo for a period of 24 hours.Conclusions: Gabapentin in the doses used was found to be effective in postoperative pain in patients undergoing planned laparoscopic cholecystectomy. It was found to be safe and no serious adverse events were reported

Emergence of Lumpy Pox Virus and Their Preventive Measures: A Global Livestock Threat

In the last few years viral disease not only affect the human but also the other life on this planet and result epidemic condition at global level. The highly transmissible viral disease called Lumpy skin disease (LSD) spread in sequential manner at worldwide level affecting the cattle or dairy animals of caprine, ovine and bovine family. The highly mutational efficiency of this viral strains affects the large-scale population and still no effective cure for it. Different vaccines designed at national and international level as a preventive measure to overcome the reoccurrence of this virus e.g., Bovivax-LSD™, Lumpivax™, Poxvac™, Lumpi-ProVacInd. However, still the mortality of LSD are low, not affect humans but emergence of the mutational variants demands novel vaccines

Micropropagation And Antimicrobial Activity Of Callicarpa Macrophylla (Priyangu) Against Medically Important Pathogens

Callicarpa macrophylla (Priyangu) a medicinally important plant, represents a class of herbal drug with very strong conceptual and traditional base. In present study extract of leaf, stem showed less antimicrobial activity than seed. They showed antimicrobial activity against three bacterial strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas and three fungal Strains Aspergillus fumigatous, Rhizopus oryzae and Aspergillus awamori. Maximum activity was observed in ethanol extract of leaf and stem, Methanol extracts of seed. Phytochemical analysis of the plant extract revealed the presence of phenol, reducing sugar, coumarin and saponins.  Micropropogation of C.macrophylla was done using stem as a explant material on MS and Woody media. Which revealed that woody media containing BAP (6-amino benzyl purine) NAA(α-napthaleneacetic acid) gave maximum proliferation  response in comparison to MS media. Plant extract (leave, stem and seed) have provide protection against RBC haemolysis and protein denaturation may act as anti-arthritic agent. We propose antiarthritic and antimicrobial activity of C.macrophylla

Emerging Threats In Aquaculture: Bibliometric Analysis Of Aeromonas Sps. As An Emerging Pathogen

The concern regarding emerging diseases in aquaculture has received considerable global interest. This research focuses on the recent emergence of Aeromonads as a possible zoonotic pathogen that can impact both human and aquatic organisms. Therefore, it is crucial to evaluate the current state of this issue. Bibliometric analysis is employed to evaluate the prevalence of Aeromonas in the field of aquaculture during the period spanning from 2004 to 2023. The investigation was performed with the VOSviewer software and the Dimension database. The present study investigates the patterns of publishing output and global collaboration throughout the last ten years. The results demonstrate a substantial and steady rise in the quantity of scholarly articles, accompanied by a noteworthy degree of collaboration among academics hailing from various nations.  This research investigates the global distribution of scholarly articles, with a specific emphasis on the countries of China and India, which have the highest levels of representation. The Chinese Academy of Fishery Science has attained the most substantial publication output among academic institutes. This study also examines the prominent academic journals, specifically Fish & Shellfish Immunology, Aquaculture, and Aquaculture Research, which serve as crucial platforms for the dissemination of scholarly informatio

Quantitative Determination and Source Variation of Rutin in Fagopyrum Tataricum

Buckwheat (Fagopyrum sp.) is a traditional crop of Uttarakhand (India) known for its high Rutin content, a bioflavonoid with significant medicinal properties. This study explores the quantitative measurement of Rutin in various buckwheat varieties grown in different regions of Uttarakhand using Thin Layer Chromatography (TLC) and High-Performance Thin Layer Chromatography (HPTLC). Twelve seed sources of Fagopyrum tataricum from the Garhwal and Kumaon regions, along with Fagopyrum esculentum seeds for comparison, were collected. Preliminary phytochemical screening of the extracts revealed the presence of flavonoids, alkaloids, tannins, and carbohydrates, with methanolic extracts showing higher concentrations. TLC analysis was employed to determine the most effective solvent system for Rutin separation, leading to the identification of three samples with substantial Rutin content. HPTLC was then used for quantitative Rutin assessment, with a calibration curve established using standard Rutin solutions. The three selected samples, along with the standard, were applied to HPTLC plates, and the Rutin content was measured based on peak areas and peak heights. The sample collected from Joshimath, Uttarakhand, known as "JOG," exhibited the highest Rutin content. This study provides valuable insights into the phytochemical composition of buckwheat varieties in Uttarakhand, which can inform the development of high-quality functional foods. Rutin, a potent antioxidant with various health benefits, underscores the significance of buckwheat as a naturally occurring functional food source. The results contribute to understanding the Rutin-rich potential of buckwheat grown in Uttarakhand and its applications in functional foods and nutraceuticals. Further research in this field can promote the cultivation and utilization of buckwheat as a source of this valuable bioactive compound with numerous health benefits

Energy from Waste: Poterioochromonas malhamensis Used for Managing Dairy Effluent and Producing Valuable Microalgal Lipid

Currently, microalgae have become a marvelous and resource-friendly alternative source of advantageous bioproducts, such as lipids, carbohydrates, proteins, or other bioactive compounds. Because of the richness of microalgae in these high-value-added metabolites, still, it is an underdeveloped source of sustainable energy and food. There are some hurdles to profitable production, such as culture contamination and costly harvesting techniques. In the current work, a chrysophyte was isolated from dairy wastewater, identified as Poterioochromonas malhamensis based on its morphology and partial 18S rRNA gene sequences. This isolate was used to remediate dairy waste water (DWW) and to obtain neutral lipids (fatty acids) from microalgae. Microalgal growth was optimized by using different concentrations of DWW, supplemented with all the nutritive requirements for better progression and flourishment. Maximum biomass yield 1.478 g L−1 was achieved by optimized cultural conditions (different concentrations of DWW with BBM media). This strain showed high nitrate and phosphate removal efficiency (87.45% and 88.96%), respectively in 15 days. The experimental results highlighted that the lipid content and the chemical oxygen demand (COD) removal were 31.60% and 88.84%, respectively, and the lipid profile of isolated microalga was C16:0, C16:1, C18:0, C18:1, and C18:2 fatty acids. For growth and treatment purposes, 75% DWW with Bold’s Basal Medium (BBM) media showed better results. This is the first report of DWW treatment using the microalga Poterioochromonas malhamensis, as far as we are aware. Its cultivation prevented the spread of pollution of freshwater sources, remedied the DWW, and generated important lipids for industry

Phytochemical Analysis And Evaluation Of Antioxidant Activity In Fagopyrum Esculentum

Herbal nutraceutical products have become increasingly popular in recent years, particularly in the dermatology and cosmetics fields, because of their potential to prevent skin photodamage and their photoprotective qualities against UV radiation. Standardized herbal extracts are necessary for modern phytopharmaceutics and phytocosmetics, and buckwheat herb, which is high in flavonoids, has shown promise as an antioxidant source. The objective of the research is to ascertain the existence of various nutraceutical components in buckwheat, such as proteins, carbohydrates, sterols, alkaloids, saponins, and tannins, both quantitatively and qualitatively. The study assesses possible interactions between these components, food, and prescribed medications as the body of research on their health benefits grows. Through a variety of phytochemical tests, the results show the high antioxidant and nutraceutical qualities of Fagopyrum esculentum, with methanolic extracts showing greater activity than water extracts. Notably, the study highlights the high antioxidant activity, alkaloids, flavonoids, tannins, and phenolic compounds of F. esculentum as potential major food supplement. This study offers a biochemical justification for its application in ethnopharmacology and as a nutraceutical to improve health and prevent a variety of ailment

Rhizospheric bacteria: the key to sustainable heavy metal detoxification strategies

The increasing rate of industrialization, anthropogenic, and geological activities have expedited the release of heavy metals (HMs) at higher concentration in environment. HM contamination resulting due to its persistent nature, injudicious use poses a potential threat by causing metal toxicities in humans and animals as well as severe damage to aquatic organisms. Bioremediation is an emerging and reliable solution for mitigation of these contaminants using rhizospheric microorganisms in an environmentally safe manner. The strategies are based on exploiting microbial metabolism and various approaches developed by plant growth promoting bacteria (PGPB) to minimize the toxicity concentration of HM at optimum levels for the environmental clean-up. Rhizospheric bacteria are employed for significant growth of plants in soil contaminated with HM. Exploitation of bacteria possessing plant-beneficial traits as well as metal detoxifying property is an economical and promising approach for bioremediation of HM. Microbial cells exhibit different mechanisms of HM resistance such as active transport, extra cellular barrier, extracellular and intracellular sequestration, and reduction of HM. Tolerance of HM in microorganisms may be chromosomal or plasmid originated. Proteins such as MerT and MerA of mer operon and czcCBA, ArsR, ArsA, ArsD, ArsB, and ArsC genes are responsible for metal detoxification in bacterial cell. This review gives insights about the potential of rhizospheric bacteria in HM removal from various polluted areas. In addition, it also gives deep insights about different mechanism of action expressed by microorganisms for HM detoxification. The dual-purpose use of biological agent as plant growth enhancement and remediation of HM contaminated site is the most significant future prospect of this article

Nano-biochar: recent progress, challenges, and opportunities for sustainable environmental remediation

Biochar is a carbonaceous by-product of lignocellulosic biomass developed by various thermochemical processes. Biochar can be transformed into “nano-biochar” by size reduction to nano-meters level. Nano-biochar presents remarkable physico-chemical behavior in comparison to macro-biochar including; higher stability, unique nanostructure, higher catalytic ability, larger specific surface area, higher porosity, improved surface functionality, and surface active sites. Nano-biochar efficiently regulates the transport and absorption of vital micro-and macro-nutrients, in addition to toxic contaminants (heavy metals, pesticides, antibiotics). However an extensive understanding of the recent nano-biochar studies is essential for large scale implementations, including development, physico-chemical properties and targeted use. Nano-biochar toxicity on different organisms and its in-direct effect on humans is an important issue of concern and needs to be extensively evaluated for large scale applications. This review provides a detailed insight on nanobiochar research for (1) development methodologies, (2) compositions and properties, (3) characterization methods, (4) potentiality as emerging sorbent, photocatalyst, enzyme carrier for environmental application, and (5) environmental concerns