Characterization of Zika virus endocytic pathways in human glioblastoma cells

Zika virus (ZIKV) infections can cause microcephaly and neurological disorders. However, the early infection events of ZIKV in neural cells remain to be characterized. Here, by using a combination of pharmacological and molecular approaches and the human glioblastoma cell T98G as a model, we first observed that ZIKV infection was inhibited by chloroquine and NH4Cl, indicating a requirement of low intracellular pH. We further showed that dynamin is required as the ZIKV entry was affected by the specific inhibitor dynasore, small interfering RNA (siRNA) knockdown of dynamin, or by expressing the dominant-negative K44A mutant. Moreover, the ZIKV entry was significantly inhibited by chlorpromazine, pitstop2, or siRNA knockdown of clathrin heavy chain, indicating an involvement of clathrin-mediated endocytosis. In addition, genistein treatment, siRNA knockdown of caveolin-1, or overexpression of a dominant-negative caveolin mutant impacted the ZIKV entry, with ZIKV particles being observed to colocalize with caveolin-1, implying that caveola endocytosis can also be involved. Furthermore, we found that the endocytosis of ZIKV is dependent on membrane cholesterol, microtubules, and actin cytoskeleton. Importantly, ZIKV infection was inhibited by silencing of Rab5 and Rab7, while confocal microscopy showed that ZIKV particles localized in Rab5- and Rab7-postive endosomes. These results indicated that, after internalization, ZIKV likely moves to Rab5-positive early endosome and Rab7-positive late endosomes before delivering its RNA into the cytoplasm. Taken together, our study, for the first time, described the early infection events of ZIKV in human glioblastoma cell T98G

Photon-generated carrier transfer process from graphene to quantum dots : optical evidences and ultrafast photonics applications

The authors acknowledge Natural Science Foundation of China (Grant Nos. 61875222, 61875223, 61605106, 11874390).Graphene/III–V semiconductor van der Waals (vdW) heterostructures offer potential access to physics, functionalities, and superior performance of optoelectronic devices. Nevertheless, the lack of a bandgap in graphene severely restricts the controllability of carrier properties and therefore impedes its applications. Here, we demonstrate the engineering of graphene bandgap in the graphene/GaAs heterostructure via C and Ga exchange induced by the method of femtosecond laser irradiation (FLI). The coupling of the bandgap-opened graphene with GaAs significantly enhances both the harvest of photons and the transfer of photon-generated carriers across the interface of vdW heterostructures. Thus, as a demonstration example, it allows us to develop a saturable absorber combining a delicately engineered graphene/GaAs vdW heterostructure with InAs quantum dots capped with short-period superlattices. This device exhibits significantly improved nonlinear characteristics including <1/3 saturation intensity and modulation depth 20 times greater than previously reported semiconductor saturable absorber mirrors. This work not only opens the route for the future development of even higher performance mode-locked lasers, but the significantly enhanced nonlinear characteristics due to doping-induced bandgap opening of graphene by FLI in the vdW heterostructures will also inspire wide applications in photonic and optoelectronic devices.Publisher PDFPeer reviewe

Intermittent QPO properties of MAXI J1820+070 revealed by Insight-HXMT

We investigate the dynamical properties of low frequency quasi-periodic oscillations (QPOs) observed from the black hole X-ray binary MAXI J1820+070 during the early part of its 2018 outburst, when the system was in a bright hard state. To this aim, we use a series of observations from the Hard X-ray Modulation Telescope Insight-HXMT, and apply a wavelet decomposition (weighted wavelet Z-transforms) to the X-ray light-curve. We find that the QPO phenomenon is intermittent within each individual observation, with some sub-intervals where the oscillation is strongly detected (high root-mean-square amplitude) and others where it is weak or absent. The average life time of individual QPO segments is ~ 5 oscillation cycles, with a 3 sigma tail up to ~ 20 cycles. There is no substantial difference between the energy spectra during intervals with strong and weak/absent QPOs. We discuss two possible reasons for the intermittent QPO strength, within the precessing jet model previously proposed for MAXI J1820+070. In the rigid precession model, intermittent QPOs are predicted to occur with a coherence Q ~ a few when the disk alignment time-scale is only a few times the precession time-scale. Alternatively, we suggest that changes in oscillation amplitude can be caused by changes in the jet speed. We discuss a possible reason for the intermittent QPO strength, within the precessing jet model previously proposed for MAXI J1820+070: we suggest that changes in oscillation amplitude are caused by changes in the jet speed. We argue that a misaligned, precessing jet scenario is also consistent with other recent observational findings that suggest an oscillation of the Compton reflection component in phase with the QPOs.Comment: 8 pages, 4 figure

Incidence of sudden unexpected death in epilepsy in community-based cohort in China

OBJECTIVE: Sudden unexpected death in epilepsy (SUDEP) is associated with the high premature mortality observed among people with epilepsy. It is, however, considered a rare event in China, probably because of lack of awareness and limitation of studies in the country. We aimed to provide some initial estimation of the burden of SUDEP in China. METHODS: We established a large Chinese community-based cohort of people with epilepsy between January 2010 and December 2011. For any participant who died during follow-up, detailed information on cause of death was obtained using a specifically designed Verbal Autopsy Questionnaire. All cases were reviewed by a multidisciplinary expert panel and reinvestigated if necessary. Sudden unexpected death in epilepsy incidence rates were estimated and case details provided. RESULTS: The cohort consisted of 1562 people and during a median 5years follow-up, 72 deaths were reported. The all-causes death incidence was 11.23 (95% CI 8.86-14.07) per 1000 person-years. Fifteen died suddenly and unexpectedly in a reasonable state of health in the week preceding death. We recorded detailed information of these 15 deaths. Thirteen were considered to be probable SUDEP and two possible SUDEP. The incidence of probable SUDEP was 2.03 (95% CI 1.13-3.38) per 1000 person-years, and the incidence of all suspected (probable and possible) SUDEP was 2.34 (95% CI 1.36-3.77) per 1000 person-years. SIGNIFICANCE: The incidence of SUDEP was relatively high among Chinese people with epilepsy when compared with that in previous community-based studies from high-income countries. The burden of SUDEP in China requires further assessments

Revealing the pseudogap in Sr3(Ru0.985Fe0.015)2O7{\mathrm{Sr}}_{3}{({\mathrm{Ru}}_{0.985}{\mathrm{Fe}}_{0.015})}_{2}{\mathrm{O}}_{7} by optical spectroscopy

We report resistivity, magnetization, and optical spectroscopy study on single-crystal sample of Sr3(Ru0.985Fe0.015 )2O7. An upturn is observed in resistivity at about 30 K. Below 30 K, the dip in resistivity R(ω), the suppression in scattering rate 1/τ (ω), the peaklike feature in optical conductivity σ1(ω), and the remainder of spectral weight all suggest the formation of a pseudogap. In addition, one phonon peak at about 600 cm−1 is distinguished at all temperatures, which has asymmetric line shape. Such asymmetric line shape can be fit by a Fano function, and the resulting Fano factor 1/q2 and linewidth γ show significant increases below 30 K, giving further evidence for the formation of a pseudogap, which might originate from the partial k-space gap opening due to density wave instability

Time evolution, cyclic solutions and geometric phases for the generalized time-dependent harmonic oscillator

The generalized time-dependent harmonic oscillator is studied. Though several approaches to the solution of this model have been available, yet a new approach is presented here, which is very suitable for the study of cyclic solutions and geometric phases. In this approach, finding the time evolution operator for the Schr\"odinger equation is reduced to solving an ordinary differential equation for a c-number vector which moves on a hyperboloid in a three-dimensional space. Cyclic solutions do not exist for all time intervals. A necessary and sufficient condition for the existence of cyclic solutions is given. There may exist some particular time interval in which all solutions with definite parity, or even all solutions, are cyclic. Criterions for the appearance of such cases are given. The known relation that the nonadiabatic geometric phase for a cyclic solution is proportional to the classical Hannay angle is reestablished. However, this is valid only for special cyclic solutions. For more general ones, the nonadiabatic geometric phase may contain an extra term. Several cases with relatively simple Hamiltonians are solved and discussed in detail. Cyclic solutions exist in most cases. The pattern of the motion, say, finite or infinite, can not be simply determined by the nature of the Hamiltonian (elliptic or hyperbolic, etc.). For a Hamiltonian with a definite nature, the motion can changes from one pattern to another, that is, some kind of phase transition may occur, if some parameter in the Hamiltonian goes through some critical value.Comment: revtex4, 28 pages, no figur