Evaluating the Ecological Sustainability of Agrifood Land in Ethnic Minority Areas: A Comparative Study in Yunnan China

Agrifood land plays a crucial role in indigenous communities. However, there is limited study on the overall sustainability and inter-ethnic comparison of agrifood lands across ethnic groups. To address these gaps, we developed a visual eco-efficiency framework of ecological footprint, biocapacity, and ecological benefit to evaluate the sustainability of agrifood land in these regions, as well as analyzed the sustainability of agrifood land and examined its explanatory factors across six ethnic groups in the Yunnan Province of China. The results showed that the ecological benefits of agrifood lands fluctuated in a low ecological deficit, and the eco-efficiency of different ethnic groups varied significantly, from 2010 to 2020. Moreover, redundancy analysis showed that cash crops, forestry, fishery, and livestock were major contributors to the eco-efficiency of agrifood lands in ethnic groups, rather than the commonly accepted staple foods. Another finding revealed that the contribution of urbanization rate to the eco-efficiency of agricultural food land had a rule of reversed U and was influenced by the annual average temperature and the ethnic population rate. Our study not only provided a visual framework for evaluating the sustainability of agrifood land in ethnic areas but also shed new light on its explanatory factors across different ethnic groups. The study served as a scientific foundation for the investigation, monitoring, and management of indigenous agriculture by governments and the agricultural sectors

Performance audit evaluation of marine development projects based on SPA and BP neural network model

Accurately grasping the comprehensive cost-benefit and influencing factors of various marine development projects is of great significance to the promotion of high-quality marine development. This work screened and constructed the performance audit evaluation index system of comprehensive cost-benefit of marine development projects from the four aspects including society, economy, resources, and environment. The entropy weight set pair analysis (SPA) was used to audit and evaluate the comprehensive cost-benefit of 15 marine development projects during the construction and operation period. The back propagation (BP) neural network model was used to test the results. The main influencing factors were analyzed by the grey relational analysis (GRA) model. It was found that the comprehensive cost-benefit performance audit evaluation index of the six projects of marine protected area and offshore wind power were higher than the other nine projects of mariculture, sewage dumping, and port industry. The main influencing factors were economic net income, loss of wetland ecological service value, environmental pollution prevention and control cost, new addition employment rate, etc. The countermeasures suggestions were put forward

TRIP13 Enhances Radioresistance of Lung Adenocarcinoma Cells through the Homologous Recombination Pathway

Background and objective Radiation therapy is one of the most common treatments for non-small cell lung cancer (NSCLC). However, the insensitivity of some tumor cells to radiation is one of the major reasons for the poor efficacy of radiotherapy and the poor prognosis of patients, and exploring the underlying mechanisms behind radioresistance is the key to solving this clinical challenge. This study aimed to identify the molecules associated with radioresistance in lung adenocarcinoma (LUAD), identified thyroid hormone receptor interactor 13 (TRIP13) as the main target initially, and explored whether TRIP13 is related to radioresistance in LUAD and the specific mechanism, with the aim of providing theoretical basis and potential targets for the combination therapy of LUAD patients receiving radiotherapy in the clinic. Methods Three datasets, GSE18842, GSE19188 and GSE33532, were selected from the Gene Expression Omnibus (GEO) database and screened for differentially expressed genes (|log FC|>1.5, P<0.05) in each of the three datasets using the R 4.1.3 software, and then Venn diagram was used to find out the differentially expressed genes common to the three datasets. The screened differential genes were then subjected to protein-protein interaction (PPI) analysis and module analysis with the help of STRING online tool and Cytoscape software, and survival prognosis analysis was performed for each gene with the help of Kaplan-Meier Plotter database, and the TRIP13 gene was identified as the main molecule for subsequent studies. Subsequently, the human LUAD cell line H292 was irradiated with multiple X-rays using a sub-lethal dose irradiation method to construct a radioresistant cell line, H292DR. The radioresistance of H292DR cells was verified using cell counting kit-8 (CCK-8) assay and clone formation assay. The expression levels of TRIP13 in H292 and H292DR cells were measured by Western blot. Small interfering RNA (siRNA) was used to silence the expression of TRIP13 in H292DR cells and Western blot assay was performed. The clone formation ability and migration ability of H292DR cells were observed after TRIP13 silencing, followed by the detection of changes in the expression levels of proteins closely related to homologous recombination, such as ataxia telangiectasia mutated (ATM) protein. Results Screening of multiple GEO datasets, validation of external datasets and survival analysis revealed that TRIP13 was highly expressed in LUAD and was associated with poor prognosis in LUAD patients who had received radiation therapy. And the results of gene set enrichment analysis (GSEA) of TRIP13 suggested that TRIP13 might be closely associated with LUAD radioresistance by promoting homologous recombination repair after radiation therapy. Experimentally, TRIP13 expression was found to be upregulated in H292DR, and silencing of TRIP13 was able to increase the sensitivity of H292DR cells to radiation. Conclusion TRIP13 is associated with poor prognosis in LUAD patients treated with radiation, possibly by promoting a homologous recombination repair pathway to mediate resistance of LUAD cells to radiation