Bolu Yöresindeki Bir Sürüde Botulismus Vakalarinin Incelenmesi

In this report, we aimed to determine the clinical, hematological and biochemical changes in Botulismus cases that were detected in a cowherd in Bolu. For this purpose, the material of the study consisted of the 10 surviving cattle after the outbreak of Botulismus and clinical, hematological and biochemical examinations of these cattle were performed. From the anamnesis, it was stated that the first symptoms occurred after 3 days in the cattle grazing on the grounds contaminated with poultry manure and carrion due to insufficient pastureland conditions. Pendant walking, muscle tremor, protruding tongue, paralysis of the tail, lying costo-sternally and paralysis in the front and hind legs were observed as clinical symptoms. All parameters were detected to be within reference values in hematological examination. In biochemical examination, serum cholesterol and aspartate aminotransferase (AST) values were found to be high while copper (Cu), iron (Fe), phosphor (P), mangan (Mn) and selenium (Se) were detected to be lower than the reference values. It was observed that hematological parameters did not change in the cattle diagnosed with Botulismus clinically. To be protected from and control Botulismus, we were of the opinion that the precautions like prevention and elimination of the source of toxin, pasture improvement, mineral supplement, proper disposal of the cadavers and vaccination should be taken

Kinkkale bölgesinde koyun kökenli echinococcus granulosus Izolatlarinin moleküler karakteri

In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCB using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, 0PB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain

Acaricidal properties of essential oils from Moroccan plants against immature ticks of Hyalomma aegyptium

Hyalomma aegyptium (Linnaeus, 1758) is a hard-tick species of the Ixodidae family with the main adult hosts being Palearctic tortoises of the genus Testudo. In Morocco, it is commonly found infecting Testudo graeca that has a wide geographic distribution and is subject to protection through international illegal trade legislation. This tortoise tick has been reported as an important vector of various human and animal pathogens. Unfortunately, to date, there are no strategies to control this hematophagous ectoparasite. The present study was conducted in laboratory to evaluate the acaricidal activity of essential oils (EOs) extracted from six plants of Moroccan origin as an alternative against H. aegyptium. All EOs tested displayed toxic effects on different life stages of H. aegyptium. Mentha suaveolens subsp. timija (LC50 = 0.910 µL/mL and LC90 = 1.465 µL/mL) and Satureja calamintha EOs (LC50 = 0.927 µL/mL and LC90 = 1.347 µL/mL) exhibited higher activity against hatching eggs, whereas Chenopodium ambrosioides EO was comparatively more toxic to larvae (LC50 = 0.444 µL/mL and LC90 = 0.918 µL/mL). Juniperus thurifera var. africana (LC50 = 0.0045 mL/cm2 and LC90 = 0.0118 mL/cm2) and Lavandula pedunculata subsp. atlantica EOs (LC50 = 0.0036 mL/cm2 and LC90 = 0.0110 mL/cm2) caused high mortality in nymphs. The effect of different EOs is discussed according to their chemical composition and bioactive components