7 research outputs found
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Direct Slicing of STEP Based NURBS Models for Solid Freeform Fabrication
Direct slicing of CAD models to generate process planning instructions for solid freeform
fabrication may overcome inherent disadvantages of using STL format in terms of the process
accuracy, ease of file management, and incorporation of multiple materials. This paper will
present the results of our development of a direct slicing algorithm for layered freeform
fabrication. The direct slicing algorithm was based on a neutral, international standard (ISO
10303) STEP-formatted NURBS geometric representation and is intended to be independent of
any commercial CAD software. The following aspects of the development effort will be
presented: 1) Determination of optimal build direction based upon STEP-based NURBS models;
2) Adaptive subdivision of NURBS data for geometric refinement; and 3) Ray-casting slice
generation into sets of raster patterns. Feasibility studies applying the direct slicing algorithm to
example models and the generation of fabrication planning instructions involving multi-material
structures will also be presented.Mechanical Engineerin
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Inflammation-induced effector CD4+ T cell interstitial migration is alpha-v integrin dependent
Leukocytes must traverse inflamed tissues to effectively control local infection. Although motility in dense tissues appears to be integrin-independent actin-myosin based, during inflammation changes to the extracellular matrix (ECM) may necessitate distinct motility requirements. Indeed, we found that T cell interstitial motility was critically dependent on RGD-binding integrins in the inflamed dermis. Inflammation-induced deposition of fibronectin was functionally linked to increased αv integrin expression on effector CD4+ T cells. Using intravital multi-photon imaging, we found that CD4+ T cell motility was dependent on αv expression. Selective αv blockade or knockdown arrested TH1 motility in the inflamed tissue and attenuated local effector function. These data show a context-dependent specificity of lymphocyte movement in inflamed tissues that is essential for protective immunity