Method for in-solution, high-throughput T1 relaxometry using fluorescent nanodiamonds

Fluorescent nanodiamonds (FNDs) have been exploited as sensitive quantum probes for nanoscale chemical and biological sensing applications, with the majority of demonstrations to date relying on the detection of single FNDs. This places significant limits on the measurement time, throughput and statistical significance of a measured result as there is usually marked inhomogeneity within FND samples. Here we have developed a measurement platform that can report the T1 spin relaxation time from a large ensemble of FNDs in solution. We first describe a refined sensing protocol for this modality and then use it to identify the optimal FND size for the detection of paramagnetic targets. Our approach is simple to set up, robust and can be used for rapid material characterisation or a variety of in-situ quantum sensing applications.Comment: 8 pages, 3 figure

How the risk of liver cancer changes after alcohol cessation: A review and meta-analysis of the current literature

<p>Abstract</p> <p>Background</p> <p>It is well established that drinking alcohol raises the risk of liver cancer (hepatocellular carcinoma). However, it has not been sufficiently established as to whether or not drinking cessation subsequently reduces the risk of liver cancer and if it does reduce the risk how long it takes for this heightened risk to fall to that of never drinkers. This question is important for effective policy design and evaluation, to establish causality and for motivational treatments.</p> <p>Methods</p> <p>A systematic review and meta-analysis using the current available evidence and a specific form of Generalised Least Squares is performed to assess how the risk of liver cancer changes with time for former drinkers.</p> <p>Results</p> <p>Four studies are found to have quantified the effect of drinking cessation on the risk of liver cancer. The meta-analysis suggests that the risk of liver cancer does indeed fall after cessation by 6-7% a year, but there remains a large uncertainty around this estimate both statistically and in its interpretation. As an illustration it is estimated that a time period of 23 years is required after drinking cessation, with a correspondingly large 95% confidence interval of 14 to 70 years, for the risk of liver cancer to be equal to that of never drinkers.</p> <p>Conclusion</p> <p>This is a relatively under researched area and this is reflected in the uncertainty of the findings. It is our view that it is not possible to extrapolate the results found here to the general population. Too few studies have addressed this question and of the studies that have, all have significant limitations. The key issue amongst the relevant studies is that it appears that current drinkers, abstainers and former drinkers are not composed of, or effectively adjusted to be, similar populations making inferences about risk changes impossible. This is a very difficult area to study effectively, but it is an important topic. More work is required to reduce both statistical uncertainty and tackle the various study limitations this paper highlights and until this is done, the current result should be considered preliminary.</p

Presynaptic External Calcium Signaling Involves the Calcium-Sensing Receptor in Neocortical Nerve Terminals

Nerve terminal invasion by an axonal spike activates voltage-gated channels, triggering calcium entry, vesicle fusion, and release of neurotransmitter. Ion channels activated at the terminal shape the presynaptic spike and so regulate the magnitude and duration of calcium entry. Consequently characterization of the functional properties of ion channels at nerve terminals is crucial to understand the regulation of transmitter release. Direct recordings from small neocortical nerve terminals have revealed that external [Ca(2+)] ([Ca(2+)](o)) indirectly regulates a non-selective cation channel (NSCC) in neocortical nerve terminals via an unknown [Ca(2+)](o) sensor. Here, we identify the first component in a presynaptic calcium signaling pathway.By combining genetic and pharmacological approaches with direct patch-clamp recordings from small acutely isolated neocortical nerve terminals we identify the extracellular calcium sensor. Our results show that the calcium-sensing receptor (CaSR), a previously identified G-protein coupled receptor that is the mainstay in serum calcium homeostasis, is the extracellular calcium sensor in these acutely dissociated nerve terminals. The NSCC currents from reduced function mutant CaSR mice were less sensitive to changes in [Ca(2+)](o) than wild-type. Calindol, an allosteric CaSR agonist, reduced NSCC currents in direct terminal recordings in a dose-dependent and reversible manner. In contrast, glutamate and GABA did not affect the NSCC currents.Our experiments identify CaSR as the first component in the [Ca(2+)](o) sensor-NSCC signaling pathway in neocortical terminals. Decreases in [Ca(2+)](o) will depress synaptic transmission because of the exquisite sensitivity of transmitter release to [Ca(2+)](o) following its entry via voltage-activated Ca(2+) channels. CaSR may detects such falls in [Ca(2+)](o) and increase action potential duration by increasing NSCC activity, thereby attenuating the impact of decreases in [Ca(2+)](o) on release probability. CaSR is positioned to detect the dynamic changes of [Ca(2+)](o) and provide presynaptic feedback that will alter brain excitability

« On-the-go » multispectral imaging system to characterize the development of vineyard foliage with quantitative and qualitative vegetation indices

EA GESTAD Agrosup CT1International audienceOver the last years, the literature presents new technologies to optimize vineyard management. In the proximal sensing context, optical sensors are mainly developed to characterize the vegetation and the most famous one is the Greenseeker RT-100 (Trimble, Germany), that provides NDVI. The interpretation of its measurements is complex because it overlaps quantitative and qualitative information. However, it is a robust active sensor especially dedicated to characterize vineyard at early growth stage. To overcome these limits, we developed a multispectral (RGB, NIR) imaging system. We present a first application of spectral imagery, in proximal sensing conditions, to characterize the vine foliage of three grapevine varieties (Meunier, Pinot Noir and Chardonnay) at four phenological stages. The imaging system is embedded on a ground vehicle acquiring images with natural light, and an original radiometric calibration is proposed. From images, three agronomic indices (NDVIimage, NDVIvegetation and “foliage occupation”) are defined. They are computed from entire images and from the area of the grapes. These indices are compared to Greenseeker ones at the beginning of berry formation to be assessed. Whatever the grapevine variety the NDVIimage is in agreement with the index provided by Greenseeker (NDVIGS). At the other stages, the comparison of NDVIGS to the other indices leads to a new interpretation of NDVIGS depending on the phenological stage. The new indices provide a better understanding on the part of quantitative and quantitative information in Greenseeker index and lead to a more accurate leaf quantity estimation (from entire images), or specific physiological status characterization

« On-the-go » multispectral imaging system to characterize the development of vineyard foliage

International audienceIn Precision Viticulture, multispectral imaging systems are currently used in remote sensing for vineyard vigor characterization but few are employed in proximal sensing. This work presents the potential of a proximal multispectral imaging system mounted on a track-laying tractor equipped with a Greenseeker RT-100 to provide an NDVI index. The camera acquired visible and near-infrared images which were calibrated in reflectance. Vegetation indices were computed and compared to Greenseeker data. From two of the resulting datasets, a spatio-temporal study of foliage description through both optical systems is presented. This first study assessed the proximal imagery regarding the similarity of results

History, Annotated Gazetteer, and Bibliography of Sarawak Ornithology

Sarawak is Malaysia’s largest state, covering most of northern Borneo. It has a remarkable history of scientific bird study, starting in the 1840s and growing ever since. To set the stage for the gazetteer, which is the core of this paper, we start with a review of this history and discuss various forces that have influenced the direction of bird research in the state. Following this introduction comes the gazetteer, which is an annotated list of c. 865 sites in Sarawak where birds have been collected, studied, or regularly observed. The gazetteer provides the latitude, longitude, and elevation of each site, and it lists publications, reports, and museum collections associated with each site. The purpose of the gazetteer is to help interested parties locate sites and investigate their research history. It is also intended to help museum curators geolocate specimens for various kinds of studies, including the assessment of bird distributions in relation to habitat change over time. A notable byproduct of the historical review and gazetteer is a bibliography of c. 750 references related to Sarawak ornithology. Another is the identification of areas in Sarawak where birds are better known and areas where they are not