Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗBluetongue (BT) is a viral non-contagious disease of ruminants which is transmitted by insects of the genus Culicoides. In recent years, BT has been a serious threat to livestock and to the economies of European countries. In Serbia the disease appeared for the first time in 2001, and after a 12 year period of freedom, it broke out again in 2014. Considering the actuality of this infectious disease, especially the need for prompt and rapid diagnostics, the aim of this paper was to determine the possibility of detecting the serological response in sheep and cattle with manifested clinical signs of the disease using two different methods: double recognition enzyme-linked immunosorbent assay (sELISA) and competitive enzyme-linked immunosorbent assay (cELISA). A total of 105 blood serum samples of cattle and sheep, which had exhibited clinical signs of BT during 2014, were taken for examination from a serum bank. Out of 74 blood serum samples of sheep and 31 blood serum samples of cattle, 52 samples of sheep and 18 samples of cattle tested positive using sELISA, while 50 samples of sheep and 18 samples of cattle gave positive reactions with cELISA. The results confirm the high sensitivity of sELISA which detected 4% more seropositive sheep in comparison with cELISA. Using Cohen’s kappa statistical analysis, almost perfect agreement was determined between the results (k>0,81) obtained by cELISA and sELISA

Provera antagonističkog delovanja mikroorganizama na Colletotrichum acutatum i Colletotrichum gloeosporioides

The antagonistic activities of five biocontrol agents: Trichoderma harzianum, Gliocladium roseum, Bacillus subtilis, Streptomyces noursei and Streptomyces natalensis, were tested in vitro against Colletotrichum acutatum and Colletotrichum gloeosporioides, the causal agents of anthracnose disease in fruit crops. The microbial antagonists inhibited mycelial growth in the dual culture assay and conidial germination of Colletotrichum isolates. The two Streptomyces species exhibited the strongest antagonism against isolates of C. acutatum and C. gloeosporioides. Microscopic examination showed that the most common mode of action was antibiosis. The results of this study identify T. harzianum, G. roseum, B. subtilis, S. natalensis and S. noursei as promising biological control agents for further testing against anthracnose disease in fruits.Antagonističko delovanje pet agenasa biološke zaštite: Trichoderma harzianum, Gliocladium roseum, Bacillus subtilis, Streptomyces noursei i Streptomyces natalensis, testirano je in vitro na Colletotrichum acutatum i Colletotrichum gloeosporioides, prouzrokovače antraknoze plodova. Svi mikrobni antagonisti inhibiraju porast micelije u testovima dvojne kultivacije i klijavost konidija Colletotrichum izolata. Dve vrste roda Streptomyces ispoljavaju najjači stepen antagonizma prema izolatima C. acutatum i C. gloeosporioides. Mikroskopsko ispitivanje pokazuje da je antibioza najčešći način delovanja antagonista. T. harzianum, G. roseum, B. subtilis, S. natalensis i S. noursei su rezultatima ovih istraživanja identifikovani kao biološki agensi koji se mogu uspešno uključiti u buduća testiranja u cilju suzbijanja antraknoze plodova

Optimization of ultrasound-assisted extraction of total polyphenolic compounds from Juglans nigra L. leaves

The ultrasound-assisted extraction of Juglans nigra L. leaves was optimized with respect to total phenolic content (TP) of the extracts by varying the concentration of aqueous ethanol solution (E) and different solvent-to-solid ratio (S). The influence and optimum of the operating parameters (E and S) was examined using response surface methodology (RSM). The statistical criteria indicated the adequacy, reliability and precision of the developed RSM model. RSM showed that maximum extraction yield of TP 28.59 mg g(-1) of draw plant was achieved at the optimal values of 50% E and 20 kg kg(-1) S. Using the modelled optimized conditions, the detected relative difference between the predicted and the experimental yield was +/- 2.3 %. The determined TP content in the extracts varied from 12.54 to 29.26 mg GAE g(-1) of dry plant indicated that J. nigra is a valuable source of phenolic substances. The extracts of J. nigra leaves obtained under optimal conditions showed good antioxidant activity (IC50 = = 18.91 +/- 0.03 mu g cm(-3)) which was determined by the scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radical. The optimization of the TP extraction process is the important step in improving techno-economics of the potential commercial preparation of J. nigra extracts, as natural source of antioxidants

Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L.

Ultrasound-assisted extraction (US-AE) of total extractive substances from the herb Lady`s Bedstraw (Galium verum L.) is useful extraction method for its application in food manufacturing. The effect of different temperatures (T) and solvent-to-solid ratios (S) on the US-AE extraction yield was studied in this paper. The influence and optimum of the operating parameters (T and S) was investigated using response surface methodology (RSM). The T and S had impact on extraction yield. RSM showed that the optimal extraction parameters, which gave a maximum extraction yield of 18.7%, were T of 40 oC and S of 30 kg kg-1. Using modeled optimized conditions, detected relative difference between predicted and experimental yield was 3.3 %. RSM successfully predicted optimal parameters and proved as useful tool for standardization of extraction conditions for bioactive components from plants important for food industry

Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L

Ultrasound-assisted extraction (US-AE) of total extractive substances from the herb Lady's Bedstraw (Galium verum L.) is useful extraction method for its application in food manufacturing. The effect of different temperatures (T) and solventto-solid ratios (S) on the US-AE extraction yield was studied in this paper. The influence and optimum of the operating parameters (T and S) was investigated using response surface methodology (RSM). The T and S had impact on extraction yield. RSM showed that the optimal extraction parameters, which gave a maximum extraction yield of 18.7%, were T of 40 oC and S of 30 kg kg(-1). Using modeled optimized conditions, detected relative difference between predicted and experimental yield was 3.3 %. RSM successfully predicted optimal parameters and proved as useful tool for standardization of extraction conditions for bioactive components from plants important for food industry

[Comparison of socio-demographic characteristics of patients with PTSD in the four countries of former Yugoslavia following the war].

OBJECTIVE: To determine the socio-demographic characteristics of survivors who presented for treatment in therapeutic centers in the four Balkan countries involved in the study by summarizing the results of STOP study ("Treatment Seeking and Treatment Outcomes in People Suffering from PTSD Following the War and Migration in the Balkans"). STOP study was performed in the following countries: Bosnia and Herzegovina (specialized center Sarajevo), Croatia (centers in Zagreb and Rijeka), Serbia and Montenegro (center in Belgrade), Germany (center in Dresden) and United Kingdom (center in London). METHODS: The authors applied a socio-demographic questionnaire in patients with PTSD who presented for treatment in the centers. RESULTS: The authors present the differences among the socio-demographic characteristics of survivors who presented for treatment in therapeutic centers in the four Balkan countries involved in the study according to: gender, status (refugee, war veterans, refugee and war veteran, civilian), ethnic background, employment status, marital status and household monthly net income. CONCLUSIONS: Comparative statistical analysis of data obtained through interviews in four countries shows significant differences across the centers concerning socio-economic characteristics of the patients

Optimization of Ultrasound-Assisted Extraction of Total Extractive Substances from Galium verum L.

Ultrasound-assisted extraction (US-AE) of total extractive substances from the herb Lady’s Bedstraw (Galium verum L.) is useful extraction method for its application in food manufacturing. The effect of different temperatures (T) and solvent-to-solid ratios (S) on the US-AE extraction yield was studied in this paper. The influence and optimum of the operating parameters (T and S) was investigated using response surface methodology (RSM). The T and S had impact on extraction yield. RSM showed that the optimal extraction parameters, which gave a maximum extraction yield of 18.7%, were T of 40oC and S of 30 kg kg-1. Using modeled optimized conditions, detected relative difference between predicted and experimental yield was 3.3 %. RSM successfully predicted optimal parameters and proved as useful tool for standardization of extraction conditions for bioactive components from plants important for food industry.Periodica Polytechnica Chemical Engineering (2017), 61(3): 200-20

Influence of Er3+/Yb3+ Concentration Ratio on the Down-conversion and Up-conversion Luminescence and Lifetime in GdVO4:Er3+/Yb3+ Microcrystals

In this paper, we studied the effects of Er3+/Yb3+ concentration ratio on structural, morphological and luminescence properties of GdVO4:Er3+/Yb3+ green phosphors prepared by a high-temperature solid state method. The samples with different concentrations (between 0.5 to 2 mol%) of dopant Er3+ emitting ions and different concentrations (between 5 to 20 mol%) of sensitizer ions (Yb3+) were studied. The phosphors were characterized by the X-ray diffraction (XRD), scanning electron microscopy (SEM) and photoluminescence spectroscopy. For all samples, XRD diffraction patterns confirmed a formation of a pure GdVO4 phase, while the SEM showed that the materials are comprised of chunks of deformed particles with an average diameter ranging from approximately 2 mu m to 8 mu m. Both, down-conversion and up-conversion emission spectra of GdVO4: Er3+/Yb3+ samples, under near UV and IR excitations, exhibit two strong emission bands in the green spectral region at 525 nm and 552 nm wavelengths corresponding to H-2(11/2) - gt I-4(15/2) and S-4(3/2) - gt I-4(15/2) electronic transitions of Er3+ ions. The intensity of the green emission was changed by changing the Er3+/Yb3+ concentration ratio. This dual-mode luminescence makes these materials ideal as green phosphors for a wide variety of applications in the fields of bioanalysis and biomedical

Регіональна політика Китаю періоду колоніальної експансії великих держав (середина ХІХ ст. – 1945 р.)

In Serbia, besides 'Pozegaca' and autochthonous plum cultivars, some cultivars developed in Cacak are used for the production of plum brandy. 'Cacanska Lepotica' and 'Cacanska Najbolja' are dessert cultivars which are being processed into brandy only in seasons when the demand for fresh fruits on the market is low. 'Cacanska Rodna' is the cultivar with combined properties, and is mainly used for the production of brandy and drying. 'Pozegaca' (which used to be a standard for brandy production) was most commonly used raw material for the production of premium-quality plum brandy. This paper presents the study of influence of plum cultivars developed in Cacak on the content of some major volatile components and sensory characteristics of plum brandy. Upon removing the stones, fruits of the studied cultivars were processed into plum brandy by the common method. The contents of methanol, 8 higher alcohols, 3 acids, 10 esters, acetaldehyde and benzaldehyde were determined in the produced plum brandies by gas chromatography. Plum brandies made from the above stated cultivars differed in the content of particular components. Methanol content in all brandies was lower than legally permitted. The highest content of higher alcohols, acids and total esters (without ethylacetate and ethyllactate) was found in brandy produced from 'Cacanska Lepotica'. The highest ethylacetate and benzaldehide content was recorded in brandy produced from 'Pozegaca'. With regard to the sensory characteristics, the brandy made from 'Cacanska Lepotica' was graded highest, whereas brandies produced from cultivars 'Pozegaca', 'Cacanska Rodna' and 'Cacanska Najbolja' followed.I BALKAN SYMPOSIUM ON FRUIT GROWIN

Supplementary data for the article: Prokić, M. D.; Borković-Mitić, S. S.; Krizmanić, I. I.; Mutić, J. J.; Trifković, J. Đ.; Gavrić, J. P.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Bioaccumulation and Effects of Metals on Oxidative Stress and Neurotoxicity Parameters in the Frogs from the Pelophylax Esculentus Complex. Ecotoxicology 2016, 25 (8), 1531–1542. https://doi.org/10.1007/s10646-016-1707-x

Supplementary material for: [https://doi.org/10.1007/s10646-016-1707-x]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2340