Tissue-Autonomous Function of Drosophila Seipin in Preventing Ectopic Lipid Droplet Formation

Obesity is characterized by accumulation of excess body fat, while lipodystrophy is characterized by loss or absence of body fat. Despite their opposite phenotypes, these two conditions both cause ectopic lipid storage in non-adipose tissues, leading to lipotoxicity, which has health-threatening consequences. The exact mechanisms underlying ectopic lipid storage remain elusive. Here we report the analysis of a Drosophila model of the most severe form of human lipodystrophy, Berardinelli-Seip Congenital Lipodystrophy 2, which is caused by mutations in the BSCL2/Seipin gene. In addition to reduced lipid storage in the fat body, dSeipin mutant flies accumulate ectopic lipid droplets in the salivary gland, a non-adipose tissue. This phenotype was suppressed by expressing dSeipin specifically within the salivary gland. dSeipin mutants display synergistic genetic interactions with lipogenic genes in the formation of ectopic lipid droplets. Our data suggest that dSeipin may participate in phosphatidic acid metabolism and subsequently down-regulate lipogenesis to prevent ectopic lipid droplet formation. In summary, we have demonstrated a tissue-autonomous role of dSeipin in ectopic lipid storage in lipodystrophy

Induction of myocarditis in rabbits injected with group A streptococci

Background & objectives: We have earlier proposed that group A streptococcal (GAS) immunoglobulin binding surface proteins (IgGBPs) might trigger anti-IgG production and immune complex formation leading to glomerulonephritis. In the present study, cardiac tissue material from rabbits injected with heat-killed GAS was investigated. Methods: Rabbits were injected intravenously with 10(9) colony forming units of streptococci three times weekly for 8 wk. Cardiac tissue samples were obtained at different times and deposition of IgG, C3, TNF-alpha and IL-6 was studied. Results: After 8 or more weeks of intravenous (iv) injections, minimal changes were seen in animals receiving an IgG non-binding GAS strain, type T27, whereas in those animals receiving either of two IgG binding GAS strains, types M1 or M22, strong inflammatory and degenerative myocardial changes accompanied by deposition of IgG and C3 were noted. Furthermore, on injecting rabbits with defined mutants of a type M22 strain, the development of myocardial tissue damage proved to. be dependent on the presence streptococcal IgGBPs. Interpretation & conclusion: The present data supported a role of streptococcal IgGBPs in the induction of myocardial tissue injury by GAS

Russian Mortality Crisis and the Quality of Vital Statistics

Russia, Ill-defined conditions, Injuries, Data quality, Vital statistics, Mortality,

Ecological signature of the end-Triassic biotic crisis: what do bivalves have to say?

In order to understand the causes underlying the Triassic-Jurassic (T/J) mass extinction, we tested different bivalve features for extinction selectivity, i.e. shell mineralogy, age at the Rhaetian and three main autoecologic traits (feeding mechanism, tiering and motility/attachment). Also, diversity and turnover rates throughout the Triassic and the Early Jurassic were analysed in detail. The dataset employed for this analysis was a precise database at genus level including data from Induan to Sinemurian times. Results point to a true mass extinction for bivalves around the T/J boundary. This extinction was not ageselective at the boundary. Certain analyses suggested that shell mineralogy was a character significantly increasing survival odds, but this relationship seems to reflect selectivity on autoecologic traits. There was no difference in extinction proportions between both feeding types (i.e. deposit feeders and filter feeders); among the other traits, deep burrowers, epifaunal-motile and endobyssate forms seem to have been favoured, while shallow burrowers (and probably reclined forms) were more heavily affected. This pattern suggests an environmental stress at the boundary with some particular issues affecting the different life modes. Models linking magmatism in the Central Atlantic Magmatic Province with the end- Triassic mass extinction are a plausible scenario for this kind of perturbation