9 research outputs found
Avaliação da administração do veneno escorpiônico da Tityus serrulatus (Lutz e Mello, 1922) sobre parâmetros comportamentais em camundongos BALB/c
Get PDFTrabalho de Conclusão de Curso, apresentado para obtenção do grau de Bacharel no curso de Ciências Biológicas, da Universidade do Extremo Sul Catarinense, UNESC.Acidente escorpiônico ou escorpionismo é o quadro de envenenamento provocado pela picada de escorpião. A espécie de escorpião Tityus serrulatus (T. serrulatus) é considerada a mais agressiva e possui uma ampla distribuição no Brasil. A maioria dos componentes identificados no veneno da T. serrulatus são as neurotoxinas; que, possuem uma ampla ação sobre o sistema nervoso central (SNC). Apesar de serem bem descritos na literatura, os efeitos do veneno deste escorpião sobre o SNC, não se sabe ao certo os efeitos comportamentais induzidos por essas toxinas. Portanto, mais estudos são necessários para avaliar os efeitos dessas neurotoxinas sobre o comportamento, o aprendizado e a memória. O objetivo pretendido com o presente estudo é avaliar os efeitos comportamentais após a administração do veneno escorpiônico da T. serrulatus em camundongos. Foram utilizados 60 camundongos Balb/c machos, adultos. Os animais foram submetidos a administração via intracerebroventricular (ICV) a mão livre do veneno da T. serrulatus, divididos em cinco grupos experimentais: LCRa (controle) e doses de 10 ng/μL, 100 ng/μL, 200 ng/μL e 300 ng/μL. Após 24h da administração do veneno, os animais foram submetidos aos testes comportamentais de campo aberto e de memória de habituação. A partir dos dados obtidos no teste de campo aberto foi observado que o veneno do escorpião não alterou a atividade locomotora, a atividade exploratória, os comportamentos estereotípicos e os comportamentos do tipo ansiosos em nenhuma das doses avaliadas. Entretanto, o veneno na dose de 100 ng/μL induziu dano na memória de habituação dos animais. Portanto, pode ser concluído que os efeitos neurotóxicos do veneno da T. serrulatus, apesar de não alterar movimentos livres e comportamento do tipo ansioso, pode levar a um dano cognitivo. Entretanto, mais estudos são necessários para melhor caracterizar os efeitos do veneno da T. serrulatus sobre o comportamento e o SNC
Effects of co-administration of lithium and celecoxib on inflammatory parameters in an animal model of mania induced by amphetamine
No full textArtigo apresentado como requisito parcial para obtenção do grau de Bacharel, no Curso de Medicina, da Universidade do Extremo Sul Catarinense- UNESC.It is observed alterations in levels of inflammatory mediatorsin Bipolar Disorder (BD) patients.Celecoxib (Cel) is an anti-inflammatory drug and can reduce levels of pro-inflammatory cytokines. The present study aimed to evaluate the effects of co-administration of lithium(Li) and Cel on inflammatory parameters in an animal model of mania induced by amphetamine. Were used Wistar rats, 60 days old (250–350g). The animals (n=10 per group) received D-amph (2mg/kg) or Sal intraperitoneally once a day, during 14 days. From the day 8–14, the animals from D-amph and Sal groups received via gavage Li (24 mg/kg), Cel (20 mg/kg), Li + Cel or water.
The behavioral analyses were evaluated through Open Field test. Furthermore, were evaluated the levels of IL-1β, IL-4, IL-10, and TNF-α. The administration of D-amph induced hyperactivity in the rats and treatment with Li plus Cel reversed these alterations. The administration of D-amph increased the IL-4, IL-10, and TNF-α levels in the serum, frontal cortex, and striatum of rats, and treatment with Li plus Cel reversed these alterations in all tissue evaluated.
The treatment with Li plus Celshowed very effective against inflammation induced by D-amph. However, more studies are necessaries to elucidate these mechanisms
Effects of co-administration of lithium and celecoxib on inflammatory parameters in an animal model of mania induced by amphetamine
No full textArtigo apresentado como requisito parcial para obtenção do grau de Bacharel, no Curso de Medicina, da Universidade do Extremo Sul Catarinense- UNESC.It is observed alterations in levels of inflammatory mediatorsin Bipolar Disorder (BD) patients.Celecoxib (Cel) is an anti-inflammatory drug and can reduce levels of pro-inflammatory cytokines. The present study aimed to evaluate the effects of co-administration of lithium(Li) and Cel on inflammatory parameters in an animal model of mania induced by amphetamine. Were used Wistar rats, 60 days old (250–350g). The animals (n=10 per group) received D-amph (2mg/kg) or Sal intraperitoneally once a day, during 14 days. From the day 8–14, the animals from D-amph and Sal groups received via gavage Li (24 mg/kg), Cel (20 mg/kg), Li + Cel or water.
The behavioral analyses were evaluated through Open Field test. Furthermore, were evaluated the levels of IL-1β, IL-4, IL-10, and TNF-α. The administration of D-amph induced hyperactivity in the rats and treatment with Li plus Cel reversed these alterations. The administration of D-amph increased the IL-4, IL-10, and TNF-α levels in the serum, frontal cortex, and striatum of rats, and treatment with Li plus Cel reversed these alterations in all tissue evaluated.
The treatment with Li plus Celshowed very effective against inflammation induced by D-amph. However, more studies are necessaries to elucidate these mechanisms
Resumo em andamento - Arquitetura e Urbanismo
No full textResumo em andamento - Arquitetura e Urbanism
NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics
No full textMammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data
Geoeconomic variations in epidemiology, ventilation management, and outcomes in invasively ventilated intensive care unit patients without acute respiratory distress syndrome: a pooled analysis of four observational studies
No full textBackground: Geoeconomic variations in epidemiology, the practice of ventilation, and outcome in invasively ventilated intensive care unit (ICU) patients without acute respiratory distress syndrome (ARDS) remain unexplored. In this analysis we aim to address these gaps using individual patient data of four large observational studies.
Methods: In this pooled analysis we harmonised individual patient data from the ERICC, LUNG SAFE, PRoVENT, and PRoVENT-iMiC prospective observational studies, which were conducted from June, 2011, to December, 2018, in 534 ICUs in 54 countries. We used the 2016 World Bank classification to define two geoeconomic regions: middle-income countries (MICs) and high-income countries (HICs). ARDS was defined according to the Berlin criteria. Descriptive statistics were used to compare patients in MICs versus HICs. The primary outcome was the use of low tidal volume ventilation (LTVV) for the first 3 days of mechanical ventilation. Secondary outcomes were key ventilation parameters (tidal volume size, positive end-expiratory pressure, fraction of inspired oxygen, peak pressure, plateau pressure, driving pressure, and respiratory rate), patient characteristics, the risk for and actual development of acute respiratory distress syndrome after the first day of ventilation, duration of ventilation, ICU length of stay, and ICU mortality.
Findings: Of the 7608 patients included in the original studies, this analysis included 3852 patients without ARDS, of whom 2345 were from MICs and 1507 were from HICs. Patients in MICs were younger, shorter and with a slightly lower body-mass index, more often had diabetes and active cancer, but less often chronic obstructive pulmonary disease and heart failure than patients from HICs. Sequential organ failure assessment scores were similar in MICs and HICs. Use of LTVV in MICs and HICs was comparable (42·4% vs 44·2%; absolute difference -1·69 [-9·58 to 6·11] p=0·67; data available in 3174 [82%] of 3852 patients). The median applied positive end expiratory pressure was lower in MICs than in HICs (5 [IQR 5-8] vs 6 [5-8] cm H2O; p=0·0011). ICU mortality was higher in MICs than in HICs (30·5% vs 19·9%; p=0·0004; adjusted effect 16·41% [95% CI 9·52-23·52]; p<0·0001) and was inversely associated with gross domestic product (adjusted odds ratio for a US$10 000 increase per capita 0·80 [95% CI 0·75-0·86]; p<0·0001).
Interpretation: Despite similar disease severity and ventilation management, ICU mortality in patients without ARDS is higher in MICs than in HICs, with a strong association with country-level economic status
