Uso de antibióticos em aquacultura e resistência bacteriana: impacto em saúde pública

Antibiotics are used in animal production, including aquaculture, to prevent and treat infectious disease. The administration of antibiotics has impact on the environment, and may lead to the emergence of antimicrobial resistance, in commensal bacteria both in human and animal gut, and in environmental bacteria, with the possible spread of resistance genes in diverse bacterial populations. The ability of horizontal transfer genes from environmental bacteria such as Aeromonas salmonicida to bacteria such as Escherichia coli has been reported.The transmission from animal to human can occur through various ways, including direct contact with animals and feces, and ingestion of food and water. The excessive and non-controlled use of antibiotics in animals has impact in public health, and transformed the antimicrobial resistance as a huge concern and a global issue. The changes in the production system can increase the importance of dissemination of resistance in the environment, as this production is becoming more integrated or more intensive in smaller geographic areas.The present review highlights the importance of the continuous monitoring of the administration of antibiotics in aquaculture.Os antibióticos têm sido utilizados em produção animal, incluindo aquacultura, para prevenção e tratamento de doenças infecciosas. A sua administração tem impacto sobre o ambiente e pode conduzir ao aparecimento de resistência antimicrobiana, tanto em bactérias comensais do intestino humano e animal, como em bactérias do meio ambiente, com a possível disseminação de genes de resistência entre diversas populações bacterianas. A capacidade de transferência horizontal de genes de resistência aos antimicrobianos de bactérias ambientais aquáticas, como Aeromonas salmonicida, para bactérias, como Escherichia coli, tem sido documentada. A transmissão do animal para o homem pode ocorrer por vários meios, incluindo contacto directo com animais e fezes, bem como ingestão de alimentos e água.O uso excessivo e não controlado de antimicrobianos em animais tem consequências na saúde pública, tendo transformado a resistência aos antimicrobianos num preocupante problema global. As mudanças no sistema de produção podem aumentar a importância da difusão de resistências no meio ambiente, se a produção se tornar mais integrada ou mais intensiva em áreas geográficas de menores dimensões.Assim, no presente trabalho realça-se a importância da monitorização contínua da administração de antibióticos em aquacultura

ACE as a Mechanosensor to Shear Stress Influences the Control of Its Own Regulation via Phosphorylation of Cytoplasmic Ser1270

Objectives: We tested whether angiotensin converting enzyme (ACE) and phosphorylation of Ser(1270) are involved in shear-stress (SS)-induced downregulation of the enzyme. Methods and Results: Western blotting analysis showed that SS (18 h, 15 dyn/cm(2)) decreases ACE expression and phosphorylation as well as p-JNK inhibition in human primary endothelial cells (EC). CHO cells expressing wild-type ACE (wt-ACE) also displayed SS-induced decrease in ACE and p-JNK. Moreover, SS decreased ACE promoter activity in wt-ACE, but had no effect in wild type CHO or CHO expressing ACE without either the extra-or the intracellular domains, and decreased less in CHO expressing a mutated ACE at Ser(1270) compared to wt-ACE (13 vs. 40%, respectively). The JNK inhibitor (SP600125, 18 h), in absence of SS, also decreased ACE promoter activity in wt-ACE. Finally, SS-induced inhibition of ACE expression and phosphorylation in EC was counteracted by simultaneous exposure to an ACE inhibitor. Conclusions: ACE displays a key role on its own downregulation in response to SS. This response requires both the extra- and the intracellular domains and ACE Ser(1270), consistent with the idea that the extracellular domain behaves as a mechanosensor while the cytoplasmic domain elicits the downstream intracellular signaling by phosphorylation on Ser(1270).Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[01/00009-0]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[03/14115-2]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[06/52053-7]Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)[480120/2007-2

The Role of miRNAs in the Prognosis of Triple-Negative Breast Cancer: A Systematic Review and Meta-Analysis

Breast cancer is one of the most common malignancies among women around the world. The basal or triple-negative subtype (TNBC) is a heterogeneous group of tumors, characterized by its aggressive and metastatic nature, with low survival and worse prognosis. Research on genetic biomarkers, such as microRNAs (miRs) in TNBC, demonstrate their relevance in the prognosis of the disease. Therefore, the objective of this research was to verify the role of miRs in the prognosis of TNBC. A search was carried out in the PubMed (MEDLINE), Web of Science, and Scopus databases, with articles in the English language from 2010 to 2022. Only articles that analyzed the role of miRNAs in the prognosis of TNBC and that met the criteria of the MOOSE method were included. For the preparation and planning of this systematic review, a PRISMA checklist and the MOOSE method were used. The Newcastle–Ottawa Scale was used to analyze the quality of the included studies. The excluded criteria considered were: (1) studies that presented duplication in the databases; (2) reviews of the literature, clinical case reports, meta-analyses, conference abstracts, letters to the editor, theses, dissertations, and book chapters; (3) studies that stratified only women diagnosed with other subtypes of breast cancer subtypes; (4) experiments without a control or comparison group. After the bibliographic survey of the 2.274 articles found, 43 articles met the inclusion criteria, totaling 5421 patients with TNBC analyzed for this review. Six miRs (miR-155, miR-21, miR-27a/b/, miR-374a/b, miR-30a/c/e, and miR-301a) were included in the meta-analysis. A low expression of miR-155 was associated with reduced overall survival (OS) (HR: 0.68, 95% CI: 0.58–0.81). A high expression of miR-21 was a predictor of OS reduction (HR: 2.56; 95% CI: 1.49–4.40). In addition, high levels of miR-27a/b and miR-301a/b were associated with lower OS, while the decreased expression levels of miR-30 and miR-374a/b were associated with worse relapse-free survival (RFS) and shorter disease-free survival (DFS), respectively. The present study revealed that miRs play essential roles in the development of metastases, in addition to acting as suppressors of the disease, thus improving the prognosis of TNBC. However, the clinical application of these findings has not yet been investigated

The Role of miRNAs in the Prognosis of Triple-Negative Breast Cancer: A Systematic Review and Meta-Analysis

Breast cancer is one of the most common malignancies among women around the world. The basal or triple-negative subtype (TNBC) is a heterogeneous group of tumors, characterized by its aggressive and metastatic nature, with low survival and worse prognosis. Research on genetic biomarkers, such as microRNAs (miRs) in TNBC, demonstrate their relevance in the prognosis of the disease. Therefore, the objective of this research was to verify the role of miRs in the prognosis of TNBC. A search was carried out in the PubMed (MEDLINE), Web of Science, and Scopus databases, with articles in the English language from 2010 to 2022. Only articles that analyzed the role of miRNAs in the prognosis of TNBC and that met the criteria of the MOOSE method were included. For the preparation and planning of this systematic review, a PRISMA checklist and the MOOSE method were used. The Newcastle–Ottawa Scale was used to analyze the quality of the included studies. The excluded criteria considered were: (1) studies that presented duplication in the databases; (2) reviews of the literature, clinical case reports, meta-analyses, conference abstracts, letters to the editor, theses, dissertations, and book chapters; (3) studies that stratified only women diagnosed with other subtypes of breast cancer subtypes; (4) experiments without a control or comparison group. After the bibliographic survey of the 2.274 articles found, 43 articles met the inclusion criteria, totaling 5421 patients with TNBC analyzed for this review. Six miRs (miR-155, miR-21, miR-27a/b/, miR-374a/b, miR-30a/c/e, and miR-301a) were included in the meta-analysis. A low expression of miR-155 was associated with reduced overall survival (OS) (HR: 0.68, 95% CI: 0.58–0.81). A high expression of miR-21 was a predictor of OS reduction (HR: 2.56; 95% CI: 1.49–4.40). In addition, high levels of miR-27a/b and miR-301a/b were associated with lower OS, while the decreased expression levels of miR-30 and miR-374a/b were associated with worse relapse-free survival (RFS) and shorter disease-free survival (DFS), respectively. The present study revealed that miRs play essential roles in the development of metastases, in addition to acting as suppressors of the disease, thus improving the prognosis of TNBC. However, the clinical application of these findings has not yet been investigated

Os direitos sexuais e o enfrentamento da violência sexual

O artigo tem como temática a problematização acerca da emergência dos direitos sexuais como direitos humanos e dos processos de criminalização contemporâneos que se materializam na violência sexual, colocando em análise suas múltiplas formas de entendimento e delimitações. Busca-se visibilizar produções de saberes - sempre articulados a relações de poder - para pôr em questão processos de subjetivação, a partir da análise do funcionamento do poder biopolítico e seus efeitos na contemporaneidade