Funneled angle landscapes for helical proteins

We use crystallographic data for four helical iron proteins (cytochrome c-b₅₆₂, cytochrome c′, sperm whale myoglobin, human cytoglobin) to calculate radial and angular signatures as each unfolds from the native state stepwise though four unfolded states. From these data we construct an angle phase diagram to display the evolution of each protein from its native state; and, in turn, the phase diagram is used to construct a funneled angle landscape for comparison with the topography of its folding energy landscape. We quantify the departure of individual helical and turning regions from the areal, angular profile of corresponding regions of the native state. This procedure allows us to identify the similarities and differences among individual helical and turning regions in the early stages of unfolding of the four helical heme proteins

Healthy Exosomes and their Effects on Diabetic Cardiomyocytes

Extracellular Vesicles, and more specifically, exosomes, are essential for effective cell-to-cell communication in a wide variety of tissues. In the last couple of decades, these nanovesicles have been proven to be active participants and regulators in many disease processes; therefore, their therapeutic effects have been widely studied and proven in various cardiovascular diseases both, in vitro and in vivo. Thus, this study aims at assessing the effects of running healthy mice exosomes on cardiomyocyte and cardiac tissue samples obtained from diabetic mice. Here, we successfully extract exosomes from mice plasma and detect their presence through the use of anti-CD9 and anti-CD81 antibodies. Further work includes concentrating exosome presence and utilizing a wider variety of exosome-specific antibodies, as well as exploring techniques for more effective exosome extraction from plasma

Supply Chain Resilience Strategies and their Impact on Sustainability: An Investigation from the Automobile Sector

This study proposes a framework comprising supply chain (SC) resilience strategies to handle low-frequency high impact (LFHI) disruptive events. It also evaluates the impact of SC resilience strategies’ implementation on the triple bottom line of SC sustainability. A hybrid three-phased method is proposed to meet the research objectives of the study. In the first phase, the study employs the Delphi technique to select SC resilience strategies and SC sustainability dimensions. In the second phase, the Best–Worst Method (BWM) is employed to assess the relative weights of resilience strategies. Finally, in the third stage, summative Likert scoring is used to understand the impact of SC resilience strategies on the SC sustainability triple bottom line (3BL).The outcomes reveal that firms give due importance to inter-organizational relationships and supplier nearness for supply continuity. In the sustainability context, the obtained scores proved that resilience strategies have the maximum impact on economic sustainability, followed by environmental sustainability. This is the first study that examines aspects of SC resilience strategies and quantifies their impact on the triple bottom line of SC sustainability. This study is specific to the automobile sector; sectoral diversity may expose similarities and dissimilarities in the approach. The outcome establishes that supplier–manufacturer relationships need to be strengthened further to tackle any future uncertainties. Besides, supplier location decisions may also be revisited. The strategies proposed will aid SC managers to make informed decisions to prepare for uncertain events. In the face of uncertain events, often SC’s trade-off sustainability in pursuit of resilience. It manifests that resilience is a prerequisite for SC sustainability. While planning SCs, organizations often choose either sustainability or resilience. Thus, this study acknowledges the need to develop effective SC resilience strategies that are in harmony with the sustainability agenda

The circular economy impact on small to medium enterprises

In recent years, the literature surrounding the circular economy has grown. While the notion of reducing, recycling and reusing have become adopted practices in many organisations under the umbrella of sustainability, having a circular economy is arguably the next generation step, in terms of sustainability. A systematic literature review on the circular economy identified a gap in the research, regarding the impact at the micro level to be placed on small to medium enterprises. The research concludes that a paradigm shift in circular thinking at the micro level is required, and that further research is needed to identify new skills, resources, approaches, and business models to enable subject matter experts (SMEs) to adopt a circular practice.N/

TREX-DM: a low background Micromegas-based TPC for low-mass WIMP detection

Dark Matter experiments are recently focusing their detection techniques in low-mass WIMPs, which requires the use of light elements and low energy threshold. In this context, we describe the TREX-DM experiment, a low background Micromegas-based TPC for low-mass WIMP detection. Its main goal is the operation of an active detection mass $\sim$0.3 kg, with an energy threshold below 0.4 keVee and fully built with previously selected radiopure materials. This work describes the commissioning of the actual setup situated in a laboratory on surface and the updates needed for a possible physics run at the Canfranc Underground Laboratory (LSC) in 2016. A preliminary background model of TREX-DM is also presented, based on a Geant4 simulation, the simulation of the detector's response and two discrimination methods: a conservative muon/electron and one based on a neutron source. Based on this background model, TREX-DM could be competitive in the search for low-mass WIMPs. In particular it could be sensitive, e.g., to the low-mass WIMP interpretation of the DAMA/LIBRA and other hints in a conservative scenario.Comment: Proceedings of the XIV International Conference on Topics in Astroparticle and Underground Physics (TAUP 2015), 7-11 September 2015, Torino, Ital

Identification of the orphan gene Prod 1 in basal and other salamander families.

The urodele amphibians (salamanders) are the only adult tetrapods able to regenerate the limb. It is unclear if this is an ancestral property that is retained in salamanders but lost in other tetrapods or if it evolved in salamanders. The three-finger protein Prod 1 is implicated in the mechanism of newt limb regeneration, and no orthologs have been found in other vertebrates, thus providing evidence for the second viewpoint. It has also been suggested that this protein could play a role in salamander-specific aspects of limb development. There are ten families of extant salamanders, and Prod 1 has only been identified in two of them to date. It is important to determine if it is present in other families and, particularly, the basal group of two families which diverged approximately 200 MYA

Integrating Industry 4.0 and Total Productive Maintenance for Global Sustainability

The integration of Total Productive Maintenance (TPM) and industry 4.0 (I4.0) is an emerging model, and the global pressure of various stakeholders raises scepticism of any emerging model towards providing sustainability. Therefore, this research aims to identify and rank the potential significant drivers of an integrated model of I4.0 and TPM to guide manufacturing enterprises towards sustainability. This research follows a four-phase methodology including literature review and expert opinion to select the sustainability indicators and I4.0 integrated TPM key drivers, followed by employing the Analytic hierarchy process (AHP) approach for weight determination of sustainability indicators. The research then deploys the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) to prioritise the I4.0 integrated TPM key drivers based on their effect on various sustainability indicators. Finally, a sensitivity analysis is conducted to check the robustness of the TOPSIS. The findings establish the top five most influential key drivers of an I4.0 integrated TPM system, which include Top management support, Formal I4.0 adoption program, Mid-management involvement and support, Solid TPM baseline knowledge, and High engagement of the production team. These top drives can lead manufacturing firms towards sustainability. The digitalisation of shop floor practices, such as TPM could be adapted by shop floor managers and policymakers of manufacturing companies to deliver sustainability-oriented outcomes. In addition, this research may aid decision-makers in the manufacturing sector in identifying the most important drivers of Industry 4.0 and TPM, which will assist them in more effectively implementing an integrated system of Industry 4.0 and TPM to practice sustainability. The scope of TPM applicability is wide, and the current research is limited to manufacturing companies. Therefore, there is a huge scope for developing and testing the integrated system of Industry 4.0 and TPM in other industrial settings, such as the textile, food and aerospace industries. This research makes a first-of-its-kind effort to examine how an I4.0 integrated TPM model affects manufacturing companies' sustainability and how such effects might be maximised

The appropriate response of Spanish Gitanos: short-run orientation beyond current socio-economic status

Humans differ greatly in their tendency to discount future events, but the reasons underlying such inter-individual differences remain poorly understood. Based on the evolutionary framework of Life History Theory, influential models predict that the extent to which individuals discount the future should be influenced by socio-ecological factors such as mortality risk, environmental predictability and resource scarcity. However, little empirical work has been conducted to compare the discounting behavior of human groups facing different socio-ecological conditions. In a lab-in-the-field economic experiment, we compared the delay discounting of a sample of Romani people from Southern Spain (Gitanos) with that of their non-Romani neighbors (i.e., the majority Spanish population). The Romani-Gitano population constitutes the main ethnic minority in all of Europe today and is characterized by lower socio-economic status (SES), lower life expectancy and poorer health than the majority, along with a historical experience of discrimination and persecution. According to those Life History Theory models, Gitanos will tend to adopt “faster” life history strategies (e.g., earlier marriage and reproduction) as an adaptation to such ecological conditions and, therefore, should discount the future more heavily than the majority. Our results support this prediction, even after controlling for the individuals’ current SES (income and education). Moreover, group-level differences explain a large share of the individual-level differences. Our data suggest that human inter-group discrimination might shape group members’ time preferences through its impact on the environmental harshness and unpredictability conditions they face

A Continuous Improvement Implementation Framework for Manufacturing Companies: a Delphi Study-Based Approach for Development and Validation

Purpose – Due to the absence of industry and region-specific implementation guides, change efforts, in particular Continuous Improvement implementations, continue to report high failure rates. The purpose of this paper is, therefore, to present a finalised implementation framework specifically tailored for manufacturing companies. Design/methodology/approach – A conceptual framework derived from the existing literature is validated, through a Delphi study, by an expert panel to review its content and suitability for a practical application. Findings – This paper proposes a finalised framework tailored to the practical needs of users. The results of the Delphi approach offer a phased implementation framework that provides an overview of the implementation process of Continuous Improvement initiatives and the key activities involved. The framework is suitable for use by both business leaders and Continuous Improvement practitioners with varying degrees of previous implementation experience. Research limitations/implications – The research was carried out in the UK and is specifically focused on the manufacturing industry. It is therefore not clear the applicability of the framework presented to other regions and industries. The framework, although validated through several phases of research, still requires practical application and testing. Originality/value – Business leaders and Continuous Improvement practitioners will benefit from having the necessary content presented in a manner that will promote its practical application. It has also been demonstrated that the framework is robust in its content, incorporating the literature findings as well as the experience of an expert panel encompassing 20 people