246 research outputs found
Modeling the influence of environmental factors on human respiratory irritation from natural exposures to Karenia brevis aerosols
Presented at CERF 2015: Grand Challenges in Coastal & Estuarine Science, Portland, Oregon, November 8 - 12, 2015 and at the Eighth Symposium on Harmful Algae in the U.S., Long Beach, California, November 15 – 19, 2015.The Coupled Natural and Human Systems program of US National Science Foundation is supporting our effort to elucidate linkages between harmful algal blooms and associated impacts on human health, society and economy of coastal communities. The human respiratory system is negatively impacted by inhaled toxic aerosols from the Florida red tide dinoflagellate, Karenia brevis. It has been hypothesized that surf height, wind speed, and wind direction were the predominant factors determining the amount of toxin reaching exposed individuals. Recent findings indicate that there are more environmental factors influencing the level of respiratory impacts from brevetoxins. We report on analyses that include additional factors such as air temperature, water temperature, dew point, barometric pressure, K. brevis cell counts, annd distance and direction from K. brevis cells to the individual. Factors contributing most to differences between no respiratory irritation and mild respiratory irritation were water temperature, air temperature, and dew point. Those factors contributing most to differences between mild respiratory irritation and moderate respiratory irritation were wind direction, surf conditions, and wind speed. Data on respiratory impacts was provided by the Beach Conditions Reporting System (BCRS). This system provides twice daily reports from lifeguards and park rangers at public beaches. These subjective reports include observed levels of respiratory irritation among beach-goers. Other environmental data were provided by a variety of local sources including private and public weather stations and many monitoring and research efforts. The resultant model provides a means to forecast respiratory impacts from observations of HAB distribution, and meteorological and oceanographic conditions.ECOHAB FL – NA06NOS478024; NSF Coupled Human and Natural Systems - # 1009106; Research Experience for Undergraduates - # 0453955; FWCC – FL Red Tide Cooperative Agreement
Human responses to Florida red tides : policy awareness and adherence to local fertilizer ordinances
Author Posting. © The Author(s), 2014. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in Science of The Total Environment 493 (2014): 898-909, doi:10.1016/j.scitotenv.2014.06.083.To mitigate the damages of natural hazards, policy responses can be beneficial only if they are
effective. Using a self-administered survey approach, this paper focuses on the adherence to
local fertilizer ordinances (i.e., county or municipal rules regulating the application of fertilizer
to private lawns or facilities such as golf courses) implemented in jurisdictions along the
southwest Florida coast in response to hazardous blooms of Florida red tides (Karenia brevis).
These ordinances play a role in the context of evolving programs of water pollution control at
federal, state, water basin, and local levels. With respect to policy effectiveness, while the
strength of physical linkages is of critical importance, the extent to which humans affected are
aware of and adhere to the relevant rules, is equally critical. We sought to understand the
public’s depth of understanding about the rationales for local fertilizer ordinances. Respondents
in Sarasota, Florida, were asked about their fertilizer practices in an area that has experienced
several major blooms of Florida red tides over the past two decades. A highly educated, older
population of 305 residents and “snowbirds” reported relatively little knowledge about a local
fertilizer ordinance, its purpose, or whether it would change the frequency, size, or duration of
red tides. This finding held true even among subpopulations that were expected to have more
interest in or to be more knowledgeable about harmful algal blooms. In the face of uncertain
science and environmental outcomes, and with individual motivations at odds with evolving
public policies, the effectiveness of local community efforts to decrease the impacts of red tides
may be compromised. Targeted social-science research on human perceptions about the risks of
Florida red tides and education about the rationales for potential policy responses is warranted.This work was funded under sponsorship of the National Science Foundation (NSF), awards
#1009106 and #1004181and the National Institute for Environmental Health Sciences (NIEHS),
award # R21ES017413-01A2. Fleming received support from the European Regional
Development Fund and European Social Fund (European Centre for Environment and Human
Health, University of Exeter Medical School)
Fretting fatigue analysis and palliatives
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering; and, (Nav.E.)--Massachusetts Institute of Technology, Dept. of Ocean Engineering, 1999.Includes bibliographical references (leaves 96-100).by Gary W. Kirkpatrick.S.M.Nav.E
Biotechnological Advances in Luciferase Enzymes
This chapter explores the history of the bioengineering advances that have been applied to common luciferase enzymes and the improvements that have been accomplished by this work. The primary focus is placed on firefly luciferase (FLuc), Gaussia luciferase (GLuc), Renilla luciferase (RLuc), Oplophorus luciferase (OLuc; NanoLuc), and bacterial luciferase (Lux). Beginning with the cloning and exogenous expression of each enzyme, their step-wise modifications are presented and the new capabilities endowed by each incremental advancement are highlighted. Using the historical basis of this information, the chapter concludes with a prospective on the overall impact these advances have had on scientific research and provides an outlook on what capabilities future advances could unlock
The human health effects of Florida Red Tide (FRT) blooms : an expanded analysis
Author Posting. © The Author(s), 2014. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in Environment International 68 (2014): 144-153, doi:10.1016/j.envint.2014.03.016.Human respiratory and digestive illnesses can be caused by exposures to brevetoxins from blooms of the marine alga Karenia brevis, also known as Florida red tide (FRT). K. brevis requires macro-nutrients to grow; although the sources of these nutrients have not been resolved completely, they are thought to originate both naturally and anthropogenically. The latter sources comprise atmospheric depositions, industrial effluents, land runoffs, or submerged groundwater discharges. To date, there has been only limited research on the extent of human health risks and economic impacts due to FRT. We hypothesized that FRT blooms were associated with increases in the numbers of emergency room visits and hospital inpatient admissions for both respiratory and digestive illnesses. We sought to estimate these relationships and to calculate the costs of associated adverse health impacts. We developed environmental exposure-response models to test the effects of FRT blooms on human health, using data from diverse sources. We estimated the FRT bloom-associated illness costs, using extant data and parameters from the literature. When controlling for resident population, a proxy for tourism, and seasonal and annual effects, we found that increases in respiratory and digestive illnesses can be explained by FRT blooms. Specifically, FRT blooms were associated with human health and economic effects in older cohorts (≥ 55 years of age) in six southwest Florida counties. Annual costs of illness ranged from 700,000 annually, but these costs could exceed 2-24 million.This research was sponsored by the National Science Foundation under NSF/CNH Grant No. 1009106.L.E. Fleming acknowledges support from the European Regional Development Fund and the European Social Fund Convergence Programme for Cornwall and the Isles of Scilly
Changes in work habits of lifeguards in relation to Florida red tide
Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Harmful Algae 9 (2010): 419-425, doi:10.1016/j.hal.2010.02.005.The marine dinoflagellate, Karenia brevis, is responsible for Florida red tides. Brevetoxins, the
neurotoxins produced by K. brevis blooms, can cause fish kills, contaminate shellfish, and lead
to respiratory illness in humans. Although several studies have assessed different economic
impacts from Florida red tide blooms, no studies to date have considered the impact on beach
lifeguard work performance. Sarasota County experiences frequent Florida red tides and staffs
lifeguards at its beaches 365 days a year. This study examined lifeguard attendance records
during the time periods of March 1 to September 30 in 2004 (no bloom) and March 1 to
September 30 in 2005 (bloom). The lifeguard attendance data demonstrated statistically
significant absenteeism during a Florida red tide bloom. The potential economic costs resulting
from red tide blooms were comprised of both lifeguard absenteeism and presenteeism. Our
estimate of the costs of absenteeism due to the 2005 red tide in Sarasota County is about 100,000 at Sarasota County beaches alone. When surveyed, lifeguards reported not
only that they experienced adverse health effects of exposure to Florida red tide but also that
their attentiveness and abilities to take preventative actions decrease when they worked during a
bloom, implying presenteeism effects. The costs of presenteeism, which imply increased risks to
beachgoers, arguably could exceed those of absenteeism by an order of magnitude. Due to the
lack of data, however, we are unable to provide credible estimates of the costs of presenteeism or
the potential increased risks to bathers.This research was supported by the National Science Foundation under The Research Experience
for Undergraduate Program, grant number 0453955; the P01 ES 10594, DHHS NIH of the
National Institute of Environmental Health Sciences; the Center for Oceans and Human Health at
the Woods Hole Oceanographic Institution [National Science Foundation (NSF) OCE-0430724;
National Institute of Environmental Health Sciences (NIEHS) P50 ES012742]; and the Ocean
and Human Health Center at the University of Miami Rosenstiel School (NSF 0CE0432368;
NIEHS 1 P50 ES12736)
Building an Effective English Support Center: A Preliminary Needs Analysis of Student English Skills and Abilities Required for Participating in an Authentic International Collaborative Event - World Youth Meeting
This is a preliminary study to assess students\u27 English language needs in terms of individual skills and the skills required for carrying out an authentic international collaborative event. Furthermore, this study will seek to clarify the role of the English Support Center, the English Lounge, in providing English language assistance both before and during this event. The role of the Center and Learning Advisor in the authentic international collaborative event has been to provide English language assistance on a drop-in basis as well as giving English presentation skills lectures at the event. This study will focus on one work group, made up of first and second year presenters, as this group requires strong English skills to prepare and deliver an English presentation in partnership with students from overseas. The study will rely on scholarly sources, emails, Facebook comments, and surveys of current and former students, interviews with senior students as well as observations by the Learning Advisor both prior to and during the W.Y.M. 2012 on August 7th and 8th. The results of this study will be used to provide direction to the English Support Center, the English Lounge, leading to improvement in the service delivery prior to and during the event. Furthermore, based on the findings this researcher wants to propose areas where English Lounge classes, activities and counseling could be modified to provide better event specific support for one group, the first year presenters and other first year students as well
Lessening the hazards of Florida red tides: a common sense approach
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hoagland, P., Kirkpatrick, B., Jin, D., Kirkpatrick, G., Fleming, L. E., Ullmann, S. G., Beet, A., Hitchcock, G., Harrison, K. K., Li, Z. C., Garrison, B., Diaz, R. E., & Lovko, V. Lessening the hazards of Florida red tides: a common sense approach. Frontiers in Marine Science, 7, (2020): 538, doi:10.3389/fmars.2020.00538.In the Gulf of Mexico, especially along the southwest Florida coast, blooms of the dinoflagellate Karenia brevis are a coastal natural hazard. The organism produces a potent class of toxins, known as brevetoxins, which are released following cell lysis into ocean or estuarine waters or, upon aerosolization, into the atmosphere. When exposed to sufficient levels of brevetoxins, humans may suffer from respiratory, gastrointestinal, or neurological illnesses. The hazard has been exacerbated by the geometric growth of human populations, including both residents and tourists, along Florida’s southwest coast. Impacts to marine organisms or ecosystems also may occur, such as fish kills or deaths of protected mammals, turtles, or birds. Since the occurrence of a severe Karenia brevis bloom off the southwest Florida coast three-quarters of a century ago, there has been an ongoing debate about the best way for humans to mitigate the impacts of this hazard. Because of the importance of tourism to coastal Florida, there are incentives for businesses and governments alike to obfuscate descriptions of these blooms, leading to the social amplification of risk. We argue that policies to improve the public’s ability to understand the physical attributes of blooms, specifically risk communication policies, are to be preferred over physical, chemical, or biological controls. In particular, we argue that responses to this type of hazard must emphasize maintaining the continuity of programs of scientific research, environmental monitoring, public education, and notification. We propose a common-sense approach to risk communication, comprising a simplification of the public provision of existing sources of information to be made available on a mobile website.The research leading to these results was supported by the US National Science Foundation (NSF) under NSF Grant No. CNH 1009106. PH and DJ acknowledge the complementary support under NSF Grant No. PFI/BIC 1534054
Rapid, High-Throughput Detection of Endocrine Disrupting Chemicals Using Autobioluminescent Cellular Bioreporters
Overexposure to endocrine disruptor chemicals (EDCs) can result in serious health problems, yet they are commonly found in everyday items such as pesticides, personal care products, nutritional supplements, and plastics. The U.S. Environmental Protection Agency, along with other such agencies from around the world, have therefore mandated that new approaches be designed to screen these products for the presence of EDCs. However, despite the presence of several types of extant EDC detection assays, there still exists a backlog approaching 87,000 chemicals currently awaiting screening. Autobioluminescent detection systems, which utilize cellular bioreporters capable of autonomously modulating bioluminescent signals without the need for external stimulation or investigator interaction, provide an attractive means for addressing this backlog because of their reduced performance costs and increased throughput relative to alternative assay systems. This chapter reviews the variety of existing EDC detection assays and evaluates the performance of a representative autobioluminescent estrogen-responsive EDC bioreporter to provide an overview of how autobioluminescence can be used to improve EDC detection using in vitro assay systems
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