1,119 research outputs found
Automated Coronal Hole Identification via Multi-Thermal Intensity Segmentation
Coronal holes (CH) are regions of open magnetic fields that appear as dark
areas in the solar corona due to their low density and temperature compared to
the surrounding quiet corona. To date, accurate identification and segmentation
of CHs has been a difficult task due to their comparable intensity to local
quiet Sun regions. Current segmentation methods typically rely on the use of
single EUV passband and magnetogram images to extract CH information. Here, the
Coronal Hole Identification via Multi-thermal Emission Recognition Algorithm
(CHIMERA) is described, which analyses multi-thermal images from the
Atmospheric Image Assembly (AIA) onboard the Solar Dynamics Observatory (SDO)
to segment coronal hole boundaries by their intensity ratio across three
passbands (171 \AA, 193 \AA, and 211 \AA). The algorithm allows accurate
extraction of CH boundaries and many of their properties, such as area,
position, latitudinal and longitudinal width, and magnetic polarity of
segmented CHs. From these properties, a clear linear relationship was
identified between the duration of geomagnetic storms and coronal hole areas.
CHIMERA can therefore form the basis of more accurate forecasting of the start
and duration of geomagnetic storms
31P NMR of Backbone Conformation and Dynamics in DNA at Cre Binding Site in Terms of Sequence Context
The Cre sequence (ACGT) is a site responsible for the binding of specific transcription factors that determine the activation of genes. Due to its major role in gene transcription, it has become a subject of immense research. The binding of transcription factors to the Cre binding site has been determined to be dependent on DNA conformation. In this study, the effects of flanking sequence around the Cre binding site on the conformation and the dynamics of DNA were investigated. The Cre binding site was studied in its native form with differing flanking sequences to determine the BI/BII profile (conformation) and the magnitude of the energy transition barrier (dynamics) between the BI and BII conformations of each phosphate step of the following three dodecamer sequences: CreACAG, CreGGAG, and CreTATA. In order to obtain the BI/BII profile of each phosphate step, 2D 31P-NMR NOESY and HSQC experiments at various temperatures were utilized. Based of the basic principles of kinetics, the lower the energy barrier between the two conformations, the easier the transition between the BI and BII conformation. Therefore, it was hypothesized that low and high %BII character lead to a large energy barrier (high ∆G‡ values), whereas average %BII character leads to a small energy barrier (low ∆G‡ values). The results of the 2D 31P-NMR experiments of the three dodecamer sequences confirmed this relationship between the %BII character and the magnitude of the energy barrier (∆G‡). However, further conformation and dynamics studies must be conducted to further understand the correlation
I Am Always Building Castles In The Air
https://digitalcommons.library.umaine.edu/mmb-vp/1633/thumbnail.jp
Distance Amplitude Correction Factors for Immersion Ultrasonic Measurements through Curved Surfaces
Near net-shaped forgings offer significant advantages for component manufacture, including less material waste and reduced costs for machining to final shape. However, curved entry surfaces on near net shape forgings create complications for ultrasonic inspection methods. In immersion ultrasonic testing, entry surface curvature causes ultrasonic beam focusing or defocusing, which affects the detection sensitivity to interior material flaws, such as voids and inclusions, as compared to inspection through planar surfaces
Functional characterization of the \u3ci\u3eArabidopsis thaliana\u3c/i\u3e orthologue of Tsc13p, the enoyl reductase of the yeast microsomal fatty acid elongating system
The protein encoded by the Arabidopsis At3g55360 gene was selected as a candidate for the enoyl reductase of the microsomal elongase system based on its homology to the Tsc13p protein of S. cerevisiae. The studies presented here demonstrate that heterologous expression of At3g55360 functionally complements the temperature-sensitive phenotype of a yeast tsc13 mutant that is deficient in enoyl reductase activity. Furthermore, AtTSC13 is shown to interact physically with the Elo2p and Elo3p components of the yeast elongase complex. At3g55360 apparently encodes the sole enoyl reductase activity associated with microsomal fatty acid elongation in Arabidopsis. Consistent with this conclusion, AtTSC13 is ubiquitously expressed in Arabidopsis
A guide to chemokines and their receptors
The chemokines (or chemotactic cytokines) are a large family of small, secreted proteins that signal through cell surface G‐protein coupled heptahelical chemokine receptors. They are best known for their ability to stimulate the migration of cells, most notably white blood cells (leukocytes). Consequently, chemokines play a central role in the development and homeostasis of the immune system, and are involved in all protective or destructive immune and inflammatory responses. Classically viewed as inducers of directed chemotactic migration, it is now clear that chemokines can stimulate a variety of other types of directed and undirected migratory behaviour, such as haptotaxis, chemokinesis, and haptokinesis, in addition to inducing cell arrest or adhesion. However, chemokine receptors on leukocytes can do more than just direct migration, and these molecules can also be expressed on, and regulate the biology of, many non‐leukocytic cell types. Chemokines are profoundly affected by post‐translational modification, by interaction with the extracellular matrix (ECM), and by binding to heptahelical ‘atypical’ chemokine receptors that regulate chemokine localisation and abundance. This guide gives a broad overview of the chemokine and chemokine receptor families; summarises the complex physical interactions that occur in the chemokine network; and, using specific examples, discusses general principles of chemokine function, focussing particularly on their ability to direct leukocyte migration
Analysis of Neighbourhoods in Multi-layered Dynamic Social Networks
Social networks existing among employees, customers or users of various IT
systems have become one of the research areas of growing importance. A social
network consists of nodes - social entities and edges linking pairs of nodes.
In regular, one-layered social networks, two nodes - i.e. people are connected
with a single edge whereas in the multi-layered social networks, there may be
many links of different types for a pair of nodes. Nowadays data about people
and their interactions, which exists in all social media, provides information
about many different types of relationships within one network. Analysing this
data one can obtain knowledge not only about the structure and characteristics
of the network but also gain understanding about semantic of human relations.
Are they direct or not? Do people tend to sustain single or multiple relations
with a given person? What types of communication is the most important for
them? Answers to these and more questions enable us to draw conclusions about
semantic of human interactions. Unfortunately, most of the methods used for
social network analysis (SNA) may be applied only to one-layered social
networks. Thus, some new structural measures for multi-layered social networks
are proposed in the paper, in particular: cross-layer clustering coefficient,
cross-layer degree centrality and various versions of multi-layered degree
centralities. Authors also investigated the dynamics of multi-layered
neighbourhood for five different layers within the social network. The
evaluation of the presented concepts on the real-world dataset is presented.
The measures proposed in the paper may directly be used to various methods for
collective classification, in which nodes are assigned to labels according to
their structural input features.Comment: 16 pages, International Journal of Computational Intelligence System
- …