18 research outputs found
Eco-friendly strategy for the joint valorization of invasive macroalgae and fast-growing wood to produce advanced biofuels
A novel sustainable scheme to jointly valorize Sargassum muticum (Sm) and Paulownia wood (PW) was proposed in this work, employing the advanced environmentally friendly microwave-assisted autohydrolysis (MA) as pretreatment. Sm is an invasive macroalga that has been drastically spread in the Atlantic coast of Europe, causing environmental damage. Conversely, Paulownia elongata x fortunei is a fast-growing biomass with a high biomass production and potential for biofuels production. Thus, the concomitant valorization of both biomasses may lead to benefits related to environmental protection and bioeconomy. A sequential approach was proposed: first stage of glucose production from Sm (treated by MA and enzymatic hydrolysis to obtain a glucose-rich liquor), and second stage with MA-pretreated PW followed by saccharification and fermentation, employing in this process the glucose rich solution obtained from algae, to obtain simultaneously second and third generation bioethanol. These approaches enabled to add the ethanol production from both biomasses, leading to up to 45.2 g ethanol/L (70% ethanol yield), boosting ethanol titers compared to using only one biomass (up to 27.8 g/L) and confirming the benefits of combining MA-processed biomass. Furthermore, up to 87% of the energy may be recovered, reflecting a suitable approach within an integrated strategy.Agencia Estatal de Investigación | Ref. PID2019-110031RB-I00Agencia Estatal de Investigación | Ref. CNS2022-136095Xunta de Galicia | Ref. ED431C 2017/62-GRCXunta de Galicia | Ref. ED481B-2022-020Agencia Estatal de Investigación | Ref. RYC2018-026177-IAgencia Estatal de Investigación | Ref. RYC2020-030690-IUniversidade de Vigo/CISU
Microwave-assisted extraction of hemicellulosic oligosaccharides and phenolics from Robinia pseudoacacia wood
Financiaciado para publicación en acceso aberto: Universidade de Vigo/CISUGMicrowave-assisted autohydrolysis is an environmentally friendly intensification technology that permits the selective solubilization of hemicelluloses in form of oligosaccharides in a short time and with low energy consumption. The purpose of this work was to evaluate the suitability of microwave-assisted autohydrolysis to produce oligosaccharides and phenolics with potential prebiotic and antioxidant activities from Robinia pseudoacacia wood. The influence of treatment time (0–30 min) and temperature (200–230 ◦C) on oligosaccharide production was studied and conditions of 230 ◦C and 0.25 min resulted in maximum content of xylooligosaccharides (7.69 g XO/L) and more efficient energy consumption. Furthermore, under those conditions, liquors showed high contents of phenols (80.28 mg GAE/g of RW) and flavonoids (44.51 RE/g) with significant antioxidant activities (112.07 and 102.30 mg TE/g, measured by ABTS and FRAP tests, respectively). Additionally, the solubilized hemicelluloses were structurally characterized by HPAEC-PAD, MALDI-TOF-MS, FTIR and TGA/DSC, and HPLC-ESI-MS analysis allowed the tentative identification of 17 phytochemicals.Ministerio de Economía y Competitividad | Ref. PID2019-110031RB-I00Xunta de Galicia | Ref. ED431C 2017/62-GRCMinisterio de Ciencia e Innovación | Ref. FPU21/02446Ministerio de Ciencia e Innovación | Ref. PRE 2020 093359Ministerio de Ciencia e Innovación | Ref. RYC2018-026177-IXunta de Galicia | Ref. ED481B-2022-02
Synergetic effect of hydrothermal and deep eutectic solvents (DES) pretreatments on Robinia wood fractionation for the manufacture of bioethanol and cellulose nanocrystals
This study dealt with the use of environmentally friendly processes based on microwave-assisted autohydrolysis (MAA) and deep eutectic solvents (DES) for the selective fractionation of Robinia pseudoacacia wood (RW) within a biorefinery approach. MAA enabled the recovery of 76% of hemicelluloses in the form of oligomers. Afterwards, different conditions were assessed for the optimal delignification of RW with the DES choline chloride combined with lactic acid reaching delignification ratios up to 86%. Two different methods were accomplished to valorize the cellulosic-rich solid fraction after delignification: (i) bioethanol via enzymatic-fermentative pathway (attaining 53.3 g ethanol/L, about 83% of ethanol yield), and (ii) cellulose nanocrystals (length of 27–550 nm, width of 2–12 nm). Hence, this study presents a novel multiproduct biorefinery to selectively separate the main components of RW and valorize its cellulosic fraction using eco-friendly proceduresUniversidade de Vigo/CISUGMinisterio de Economía| Ref. PID2019-110031RB-I00Xunta de Galicia | Ref. ED431C 2017/62-GRCMinisterio de Ciencia, Innovación y Universidades | Ref. FPU21/02446Ministerio de Ciencia, Innovación y Universidades | Ref. PRE2020-093359Ministerio de Ciencia, Innovación y Universidades | Ref. RYC2018-026177-IXunta de Galicia | Ref. ED481B-2022-02
Co-valorization of discarded wood pinchips and sludge from the pulp and paper industry for production of advanced biofuels
Several lignocellulosic wastes are generated in the pulp and paper industry (PPI), such as small wood chips (pinchips) and paper sludge, presenting a high cellulose content suitable to be converted into biofuels or bio-products in a forest biorefinery scheme. In this work, two schemes of biorefinery were proposed for their valorization, processing small eucalyptus wood pinchips in two different strategies: (i) autohydrolysis at 230ºC, and (ii) autohydrolysis at 195ºC followed by organosolv process (47.7% ethanol-water, 198ºC for 60 min). More than 95% of cellulose was recovered in both schemes. In the combined process, 76% of delignification was achieved and 78% of xylan was solubilized as xylooligosaccharides. To reduce operational cost of lignocellulosic biomass-to-ethanol fermentation, the mixture of the treated eucalyptus pinchips from two processes with sludge was also proposed to increase the initial glucan content and to supply a rich source of nitrogen (present in the sludge). For that, two experimental designs were carried out for ethanol production by simultaneous saccharification and fermentation (SSF) process. Ethanol from SSF assays using sludge as co-substrate at 0.6 g of sludge/g of treated wood pinchips and 16 FPU/g of pretreated solids allowed to obtain 59 g/L (90% of conversion) and 46 g/L (96% of conversion) when blended with the wood from autohydrolysis and with the wood from autohydrolysis followed by organosolv, respectively. Overall, this study shows an alternative process valorization of biomasses derived from PPI for production of advanced biofuels and bio-products (such as xylooligosaccharides and lignin) contributing to achieving a circular economy.Xunta de Galicia | Ref. ED431C 2017/62Xunta de Galicia | Ref. ED431F 2022/09Xunta de Galicia | Ref. ED481B-2022-020Agencia Estatal de Investigación | Ref. RYC2020-030690-IUniversidade de Vigo/CISU
Recuperacion de compostos bioactivos procedentes de podas de vide mediante o uso de disolventes intelixentes
Neste traballo investigouse a obtención de compostos antioxidantes a partir de podas da vide mediante extracción sólido-líquido utilizando solventes eutécticos profundos (DES) como un medio para desenvolver procesos de separación sostibles. A partir dunha selección preliminar de sete Deep Eutectic Solvents (DES) diferentes, seleccionouse unha combinación a base de glicerina-glicina-auga como sistema máis efectivo para recuperar compostos fenólicos a partir desta biomasa residual. Posteriormente, avaliouse o efecto de diferentes parámetros operativos como a temperatura (T), tempo (t), contido en auga e relación líquido-sólido (RLS) para determinar as condicións óptimas de extracción para recuperar antioxidantes naturais en termos de contido fenolóxico total (TPC), flavonoides (TFC) e actividade antioxidante dos extractos. As condicións óptimas (T = 70 ºC, t = 45 min, 40 % de auga e RLS = 15 mL/g) permitiron a recuperación dun alto nivel de compostos fenólicos (TPC = 27,49 mg GAE/g biomasa) e de flavonoides (TFC = 40,74 mg RE/g biomasa); así como unha notable actividade antioxidante (29,76; 36,09 e 20,51 mg TE/g biomasa para os ensaios de DPPH, ABTS e FRAP, respectivamente)
Recent advances in biorefineries based on lignin extraction using deep eutectic solvents: A review
Considering the urgent need for alternative biorefinery schemes based on sustainable development, this review aims to summarize the state-of-the-art in the use of deep eutectic solvent pretreatment to fractionate lignocellulose, with a focus on lignin recovery. For that, the key parameters influencing the process are discussed, as well as various strategies to enhance this pretreatment efficiency are explored. Moreover, this review describes the challenges and opportunities associated with the valorization of extraction-derived streams and highlights recent advancements in solvent recovery techniques. Furthermore, the utilization of computational models for process design and optimization is introduced, as the initial attempts at the economic and environmental assessment of this lignocellulosic bioprocess based on deep eutectic solvents. Overall, this review offers a comprehensive perspective on the recent advances in this emerging field and serves as a foundation for further research on the potential integration of deep eutectic pretreatment in sustainable multi-product biorefinery schemes.Agencia Estatal de Investigación | Ref. PID2019-110031RB-I00Agencia Estatal de Investigación | Ref. TED2021-132088B-I00Xunta de Galicia | Ref. ED431C 2021/46-GRCXunta de Galicia | Ref. ED431F 2022/09 k251Universidade de Vigo/CISU
Optimization the soda-AQ process for cellulose pulp production and energy content of black liquor from L. leucocephala K360
A commercial variety of Leucaena leucocephala K360 was used for pulp production and papermaking employing the soda-anthraquinone process. Also, the chemical and energy contents of the resultant black liquors were determined to simultaneously optimize: pulp and paper production and energy generation. A process temperature of (185 °C), an operating time of (120 min) and an active alkali concentration of (21%) provided sheets of paper with good strength (tensile index of 12.12 N m/g, burst index of 0.38 kPa m2/g, tear index of 1.29 mN m2/g and a Kappa number of 20.5) and black liquor with a greater calorific value (14.1 MJ/kg) than that obtained with higher active alkali concentrations.
However, reducing the active alkali concentration to a level in the low operation range led to less marked degradation of cellulose and allowed paper sheets with good properties to be obtained and energy to be optimally produced from the black liquor
Association of Candidate Gene Polymorphisms With Chronic Kidney Disease: Results of a Case-Control Analysis in the Nefrona Cohort
Chronic kidney disease (CKD) is a major risk factor for end-stage renal disease, cardiovascular disease and premature death. Despite classical clinical risk factors for CKD and some genetic risk factors have been identified, the residual risk observed in prediction models is still high. Therefore, new risk factors need to be identified in order to better predict the risk of CKD in the population. Here, we analyzed the genetic association of 79 SNPs of proteins associated with mineral metabolism disturbances with CKD in a cohort that includes 2, 445 CKD cases and 559 controls. Genotyping was performed with matrix assisted laser desorption ionizationtime of flight mass spectrometry. We used logistic regression models considering different genetic inheritance models to assess the association of the SNPs with the prevalence of CKD, adjusting for known risk factors. Eight SNPs (rs1126616, rs35068180, rs2238135, rs1800247, rs385564, rs4236, rs2248359, and rs1564858) were associated with CKD even after adjusting by sex, age and race. A model containing five of these SNPs (rs1126616, rs35068180, rs1800247, rs4236, and rs2248359), diabetes and hypertension showed better performance than models considering only clinical risk factors, significantly increasing the area under the curve of the model without polymorphisms. Furthermore, one of the SNPs (the rs2248359) showed an interaction with hypertension, being the risk genotype affecting only hypertensive patients. We conclude that 5 SNPs related to proteins implicated in mineral metabolism disturbances (Osteopontin, osteocalcin, matrix gla protein, matrix metalloprotease 3 and 24 hydroxylase) are associated to an increased risk of suffering CKD
Fast-growing Paulownia wood fractionation by microwave-assisted hydrothermal treatment: a kinetic assessment
Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGMicrowave hydrothermal treatment (MHT), a novel advanced technology, was proposed for the fractionation of Paulownia wood (PW) at temperatures ranging 200–230 °C and residence times of 0–50 min, corresponding to severities of 2.93–4.70. This procedure allowed 80% of xylan recovery as xylooligosaccharides and an average of 95% cellulose recovery in the pretreated PW biomass, showing the selectivity of the treatment, that was also compared to conduction–convection heating autohydrolysis. Finally, a kinetic model was proposed for the prediction of PW fractionation using MHT, with the ultimate goal of being applied to a wide range of feedstocks and minimizing the number of parameters used. For that, two strategies were approached, allowing the reduction of 80 to 34 parameters, without significant influence in the kinetic fitting. To the best of our knowledge, this is the first kinetic modelization of MHT of PW, taking into account all the lignocellulosic fractions.Agencia Estatal de Investigación | Ref. PID2019-110031RB-I00Ministerio de Economía y Competitividad (España) | Ref. CTM2015-68503-RMinisterio de Educación, Cultura y Deporte (España) | Ref. FPU16/04077Ministerio de Ciencia, Innovación y Universidades (España) | Ref. RYC2018-026177-IXunta de Galicia | Ref. ED431C 2017/6
Manufacturing of hemicellulosic oligosaccharides from fast-growing Paulownia wood via autohydrolysis: Microwave versus conventional heating
Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGMicrowave hydrothermal treatment (MHT) is considered a sustainable technology for the valorization of lignocellulosic materials, enabling the solubilization of hemicellulosic-derived compounds, especially in the form of oligosaccharides that may present potential in the chemical, pharmaceutical or nutraceutical industries. Hence, MHT at 200 and 230 °C, at severity (S0) among 2.92–4.66 were performed. S0 = 3.98 permitted the recovery of about 80% of the initial xylan as xylooligosaccharides. In order to compare the effectiveness of MHT, conventional hydrothermal treatment (CHT) was performed at conditions leading to the maximum recovery of oligosaccharides (S0 =3.98, non-isothermal regime at 203 °C). Despite the structural features of oligomers in the three liquors were very similar, the spent solids presented different enzymatic digestibility, which implied a different effect of the treatments, reaching up to 80% of glucan to glucose conversion for the solid after MHT at 230 °C for 0.5 min. Additionally, CHT consumed 2.1–2.8-fold greater energy than MHT, reflecting that microwave-assisted autohydrolysis is a sustainable and efficient technology to process PW.Agencia Estatal de Investigación | Ref. PID2019-110031RB-I00Ministerio de Educación, Cultura y Deporte | Ref. FPU16/04077Agencia Estatal de Investigación | Ref. RYC2018-026177-IXunta de Galicia | Ref. ED431C 2017/6