China-EU Energy Security Cooperation: The Case for Renewables

The idea of China and the European Union cooperating on energy security is one which may not seem logical or likely at first glance. The two actors are geographically distant from one another and their energy supply needs and routes do not intersect for the most part. Yet careful study of the possibilities reveals that there is not only the potential, but also the need for cooperation on energy security. This is particularly the case in the field of renewables, where there is an opportunity to achieve win-win synergies which can produce enhanced outcomes in terms of local and global energy production and consumption.Publication is a part of the project: Regional Center for International Debate in Lodz 2017–2018 Project co-financed by the Polish Ministry of Foreign Affair

The Effect of Oral Leucine on Protein Metabolism in Adolescents with Type 1 Diabetes Mellitus

Lack of insulin results in a catabolic state in subjects with insulin-dependent diabetes mellitus which is reversed by insulin treatment. Amino acid supply, especially branched chain amino acids such as leucine, enhances protein synthesis in both animal and human studies. This small study was undertaken to assess the acute effect of supplemental leucine on protein metabolism in adolescents with type 1 diabetes. L-[1-13C] Leucine was used to assess whole-body protein metabolism in six adolescent females (16–18 yrs) with type 1 diabetes during consumption of a basal diet (containing 58 μmoles leucine/kg/h) and the basal diet with supplemental leucine (232 μmoles leucine/kg/h). Net leucine balance was significantly higher with supplemental leucine (56.33 ± 12.13 μmoles leucine/kg body weight/hr) than with the basal diet (−11.7 ± −5.91, P < .001) due to reduced protein degradation (49.54 ± 18.80 μmoles leucine/kg body weight/hr) compared to the basal diet (109 ± 13.05, P < .001)

The Belt and Road Initiative and the Eurasian Economic Union: Exploring the "Greater Eurasian Partnership"

The Russian-led Eurasian Economic Union (EAEU) is a key partner in China's Belt, and Road Initiative (BRI), since it comprises the majority of territories which the BRI’s overland route, the Silk Road Economic Belt, needs to traverse as it crosses Central Asia on the way to Europe. The goal of this article is to explore the BRI in the context of BRI-EAEU coordination. The first part of the analysis focusses on the ways the Eurasian Economic Commission delineates the "Greater Eurasian Partnership" and counterposes it against China and the BRI. Then, the article compares two sets of interpretations of the BRI and "Greater Eurasian Partnership" obtained from interviews with elites in Kazakhstan and Russia. The interviews indicate that the BRI has had a much more forceful impact on local elites than Russia's idea of "Greater Eurasian Partnership.

China's "Belt and Road" Economic Diplomacy in the Persian Gulf: Strategic Hedging amidst Saudi-Iranian Regional Rivalry

Drawing on the literature on strategic hedging and adapting it to China’s use of economic diplomacy in the service of comprehensive national security goals within the regionalised foreign policy approach of the Belt and Road Initiative (BRI), we examine China’s approach to securing and expanding its interests in the Persian Gulf. To implement the trade and infrastructure connectivity goals of the BRI and to secure the continued flow of diversified energy supplies, China needs to boost relations with both regional powerhouses, Iran and Saudi Arabia, without alienating either of them or the regional hegemon, the United States. The resulting strategy of strategic hedging is based in the Chinese approach to economic diplomacy, which utilises Chinese commercial actors in the service of national strategic objectives. Relations require careful and ongoing management if China is to achieve outcomes which benefit all sides while avoiding becoming entangled in the region’s intractable geopolitical problems

Binding of Filamentous Actin to Anthrax Toxin Receptor 1 Decreases Its Association with Protective Antigen

ANTXR1 is a type I membrane protein that binds the protective antigen (PA) component of anthrax toxin. The cytosolic domain of ANTXR1 has a novel actin-binding region that influences the interaction of the ectodomain with PA. Here, we have investigated features of the cytosolic domain of ANTXR1 that reduce the association of the receptor with PA. We mutated a stretch of conserved acidic amino acids adjacent to the actin-binding region and found that the mutation increased the affinity for monomeric actin in vitro. ANTXR1 bearing this mutation exhibited increased association with the cytoskeleton and bound less PA compared to the wild-type receptor, confirming the inverse correlation between the two interactions. To determine whether binding of actin is sufficient to regulate the ectodomain, we replaced the actin-binding region of ANTXR1 with that from the yeast protein abp140 and with the WH2 domain of WAVE2. Although both of these domains bound monomeric actin in vitro, only the sequence from abp140 reduced binding of PA to a hybrid receptor. The actin binding regions of ANTXR1 and abp140, but not the WH2 domain, colocalized with actin stress fibers, which suggested that filamentous actin regulates ANTXR1. Consistent with this notion, disruption of actin filaments using latrunculin A increased the amount of PA bound to cells. This work provides evidence that cytoskeletal dynamics regulate ANTXR1 function

Imaging nanoscale nuclear structures with expansion microscopy

Commonly applied super-resolution light microscopies have provided insight into subcellular processes at the nanoscale. However, imaging depth, speed, throughput and cost remain significant challenges, limiting the numbers of three-dimensional (3D) nanoscale processes that can be investigated and the number of laboratories able to undertake such analysis. Expansion microscopy (ExM) solves many of these limitations, but its application to imaging nuclear processes has been constrained by concerns of unequal nuclear expansion. Here, we demonstrate the conditions for isotropic expansion of the nucleus at a resolution equal to or better than 120–130 nm (pre-expansion). Using the DNA damage response proteins BRCA1, 53BP1 (also known as TP53BP1) and RAD51 as exemplars, we quantitatively describe the 3D nanoscale organisation of over 50,000 DNA damage response structures. We demonstrate the ability to assess chromatin-regulated events and show the simultaneous assessment of four elements. This study thus demonstrates how ExM can contribute to the investigation of nanoscale nuclear processes

Imaging nanoscale nuclear structures with expansion microscopy

Commonly applied super-resolution light microscopies have provided insight into subcellular processes at the nanoscale. However, imaging depth, speed, throughput and cost remain significant challenges, limiting the numbers of three-dimensional (3D) nanoscale processes that can be investigated and the number of laboratories able to undertake such analysis. Expansion microscopy (ExM) solves many of these limitations, but its application to imaging nuclear processes has been constrained by concerns of unequal nuclear expansion. Here, we demonstrate the conditions for isotropic expansion of the nucleus at a resolution equal to or better than 120–130 nm (pre-expansion). Using the DNA damage response proteins BRCA1, 53BP1 (also known as TP53BP1) and RAD51 as exemplars, we quantitatively describe the 3D nanoscale organisation of over 50,000 DNA damage response structures. We demonstrate the ability to assess chromatin-regulated events and show the simultaneous assessment of four elements. This study thus demonstrates how ExM can contribute to the investigation of nanoscale nuclear processes