Comparison of dietary agents’ garlic and bitter melon on in vitro glycation and advanced glycation end products formation

Background: Protein glycation is a spontaneous post translational modification of proteins by excess sugars causing formation of advanced glycation end products (AGEs) in diabetic individuals and responsible for diabetes complications. Momordica charantia L (bitter melon) and garlic have been used historically for medicinal purposes particularly for treatment of diabetes and cancers and contain potent antioxidant activity hence we planned to compare the antiglycating activities.Methods: Human serum albumin (HSA) was used for in vitro glycation. Various concentrations of extracts of M. charantia L and aged garlic were analyzed.Results: Co-incubation of the M. charantia L and aged garlic extracts with HSA-fructose mixture gives contradictory results in tryptophan fluorescence, AGE specific fluorescence and protein bound carbonyl studies.Conclusions: M. charantia L seems to aggravate sugar mediated glycation of the protein and need further studies to pinpoint specific bioactive compounds responsible for the observed activities whereas aged garlic seems to have strong ant glycation properties

Anti-dyslipidemic activity of acacia tortilis seed extract in alloxan-induced diabetic rats

Background: The present study was carried out to evaluate the anti-dyslipidemic activities of seed extract of acacia tortilis (ATE) in alloxan inducd diabetic rats.Methods: The Rats were divided into five groups of six animals each. Groups I and II received normal saline, group III received ATE in dose of 100 mg/kg body weight, group IV received ATE in dose of 200 mg/kg b.w.; and group V received standard drug pioglitazone dose 3 mg/kg b.w. Drugs were administered orally once a day for 30 days. At the end of 0th, 10th, 20th  and 30th day, blood was collected to analyse serum glucose, serum insulin, total cholesterol (TC), serum phospholipid (PL), serum triglyceride (TG), Free fatty acids (FFA) and High density lipoprotein (HDL).Results: The results has been showed that ATE in above doses significantly increase the serum insulin and HDL level but significantly decreased the elevated level of TC, PL, TG , FFA, LDL and VLDL. It also decreased the atherogenic index and coronary risk index level significantly which was comparable with the pioglitazone.Conclusions: It is concluded that the seed extract of acacia tortilis at the dose of 100 and 200 mg/kg body weight produced significant anti-dyslipidemic activity in alloxan-induced diabetic rats

ASSESSMENT OF KNOWLEDGE AND AWARENESS ABOUT LEPROSY AMONG MEDICAL COLLEGE STUDENTS IN MEWAT REGION OF HARYANA

Introduction -Leprosy is a chronic infectious disease which still remains a serious public health problem due to its ability to cause disability. The prevention of leprosy ultimately lies in the early diagnosis and treatment of the individuals having leprosy. Changing leprosy scenario has led to requirement of leprosy education. However the knowledge of medical college students on leprosy remains unknown. Aim: To assess the post-sensitization knowledge and awareness of medical students after state leprosy sensitization program. Materials and methods: A questionnaire based, cross sectional study was conducted among 107 medical students of different years starting from 2nd year and onwards in the month of July 2017 after state leprosy sensitization program. Results: Among the medical students 70 (75%) were male students and 37(25%) were female students coming from different years of their admission in medical college .Regarding the basic knowledge of leprosy 100 % of 3nd year and final year students were having knowledge about the cause of leprosy and 100% of final year and 3rd year medical students know about other name of Leprosy (Hansen,s disease) respectively.100% of final year, 3rd year and 2nd year medical students had knowledge about multidrug therapy (MDT) in in-depth knowledge of leprosy respectively. Regarding the virulence of bacilli 87%, 60% and 63% of final, 3rd and 2nd year medical students had apt knowledge respectively. Conclusion-These observations suggest that the basic and in-depth knowledge of medical students about cause, types, prevention and treatment etc. of leprosy shows increasing trend owing to the part of their curriculum. However there is still a need to organize sensitization programs at regular intervals to impart basic and in-depth knowledge to new medical students, as well as to update the knowledge of those who already had such sensitization programs.Keywords: Awareness; Knowledge; Leprosy; Sensitization

ASSESSMENT OF KNOWLEDGE AND AWARENESS ABOUT LEPROSY AMONG MEDICAL COLLEGE STUDENTS IN MEWAT REGION OF HARYANA

Introduction -Leprosy is a chronic infectious disease which still remains a serious public health problem due to its ability to cause disability. The prevention of leprosy ultimately lies in the early diagnosis and treatment of the individuals having leprosy. Changing leprosy scenario has led to requirement of leprosy education. However the knowledge of medical college students on leprosy remains unknown. Aim: To assess the post-sensitization knowledge and awareness of medical students after state leprosy sensitization program. Materials and methods: A questionnaire based, cross sectional study was conducted among 107 medical students of different years starting from 2nd year and onwards in the month of July 2017 after state leprosy sensitization program. Results: Among the medical students 70 (75%) were male students and 37(25%) were female students coming from different years of their admission in medical college .Regarding the basic knowledge of leprosy 100 % of 3nd year and final year students were having knowledge about the cause of leprosy and 100% of final year and 3rd year medical students know about other name of Leprosy (Hansen,s disease) respectively.100% of final year, 3rd year and 2nd year medical students had knowledge about multidrug therapy (MDT) in in-depth knowledge of leprosy respectively. Regarding the virulence of bacilli 87%, 60% and 63% of final, 3rd and 2nd year medical students had apt knowledge respectively. Conclusion-These observations suggest that the basic and in-depth knowledge of medical students about cause, types, prevention and treatment etc. of leprosy shows increasing trend owing to the part of their curriculum. However there is still a need to organize sensitization programs at regular intervals to impart basic and in-depth knowledge to new medical students, as well as to update the knowledge of those who already had such sensitization programs.Keywords: Awareness; Knowledge; Leprosy; Sensitization

Comparison of dietary agents' garlic and bitter melon on in vitro glycation and advanced glycation end products formation

Background: Protein glycation is a spontaneous post translational modification of proteins by excess sugars causing formation of advanced glycation end products (AGEs) in diabetic individuals and responsible for diabetes complications. Momordica charantia L (bitter melon) and garlic have been used historically for medicinal purposes particularly for treatment of diabetes and cancers and contain potent antioxidant activity hence we planned to compare the antiglycating activities.Methods: Human serum albumin (HSA) was used for in vitro glycation. Various concentrations of extracts of M. charantia L and aged garlic were analyzed.Results: Co-incubation of the M. charantia L and aged garlic extracts with HSA-fructose mixture gives contradictory results in tryptophan fluorescence, AGE specific fluorescence and protein bound carbonyl studies.Conclusions: M. charantia L seems to aggravate sugar mediated glycation of the protein and need further studies to pinpoint specific bioactive compounds responsible for the observed activities whereas aged garlic seems to have strong ant glycation properties

Effect of Good Clinical Laboratory Practices Quality Training on Knowledge, Attitude and Practice among Laboratory ProfessionalsQuasi Experimental Study

Introduction: Good Clinical Laboratory Practices (GCLP) play a vital role in early and accurate diagnosis, providing highquality data, and timely sample processing. Adhering to a robust Quality Management System (QMS) that complies with GCLP standards is crucial for laboratory personnel in a clinical laboratory to deliver outstanding healthcare services and reliable, reproducible reports. Aim: To assess the Knowledge, Attitude, and Practice (KAP) of laboratory professionals towards quality in the laboratory through GCLP training. Materials and Methods: This pre-test, post-test quasiexperimental study was conducted in the Department of Biochemistry at ESIC Medical College and Hospital, Faridabad, Haryana, India, from February 2022 to June 2022. The study included 58 participants, consisting of 22 doctors and the remaining laboratory assistants. GCLP online training program was conducted every Friday in March 2022 for four weeks. An online questionnaire containing 34 questions was administered to all the participants before and after the training. Data were collected and analysed using a paired t-test. Results: A total of 58 responses were received from the participants via Google form before and after the training. The results indicate no significant difference in participants’ responses to 12 closed-ended questions regarding QMS before and after training. A similar trend was observed for 22 questions on a Likert scale, where participants rated their agreement, neutrality, or disagreement. Conclusion: The study demonstrates that all technical staff fully complied with GCLP guidelines and accreditation requirements. Furthermore, the laboratory staff acknowledges the importance of Standard Operating Procedures (SOPs), document maintenance, record-keeping, and identifying nonconformities, all of which contribute to effective traceability of the testing process in the clinical laboratory