The distribution and population ecology of semaphore crab Ilyoplax sayajiraoi Trivedi, Soni, Trivedi and Vachhrajani 2015, on mud flat region of Gulf of Khambhat, Gujarat, India

The semaphore crab Ilyoplax sayajiraoi is a newly discovered species distributed in the Gulf of Khambhat, Gujarat, India. Consequently, almost no information is available regarding its population structure. The distribution of species is limited to upper most reaches of the gulf and thus any alteration in the habitat will eventually affect its population. Studies on the population structure of I. sayajiraoi will provide baseline data for future studies and probably, it has potential to serve as a bioindicator species. Morphometric measurements of the carapace (width and length), cheliped and body weight were measured for specimens, collected monthly. The growth was not isometric, demonstrating a significant difference in carapace of males and females, giving sexual dimorphism. Relationship established between carapace width and weight indicate that species is allometric in nature. Sex ratio was 1.3 : 1 (M : F).  Ovigerous female and juvenile recruitment showed a bimodal distribution pattern for maintaining the overall size of population

Genetic, Biochemical, and Molecular Characterization of Methanosarcina barkeri Mutants Lacking Three Distinct Classes of Hydrogenase

The methanogenic archaeon Methanosarcina barkeri encodes three distinct types of hydrogenase, whose functions vary depending on the growth substrate. These include the F_(420)-dependent (Frh), methanophenazine-dependent (Vht), and ferredoxin-dependent (Ech) hydrogenases. To investigate their physiological roles, we characterized a series of mutants lacking each hydrogenase in various combinations. Mutants lacking Frh, Vht, or Ech in any combination failed to grow on H_2-CO_2, whereas only Vht and Ech were essential for growth on acetate. In contrast, a mutant lacking all three grew on methanol with a final growth yield similar to that of the wild type and produced methane and CO2 in the expected 3:1 ratio but had a ca. 33% lower growth rate. Thus, hydrogenases play a significant, but nonessential, role during growth on this substrate. As previously observed, mutants lacking Ech failed to grow on methanol-H_2 unless they were supplemented with biosynthetic precursors. Interestingly, this phenotype was abolished in the Δech Δfrh and Δech Δfrh Δvht mutants, consistent with the idea that hydrogenases inhibit methanol oxidation in the presence of H_2, which prevents production of the reducing equivalents needed for biosynthesis. Quantification of the methane and CO_2 produced from methanol by resting cell suspensions of various mutants supported this conclusion. On the basis of the global transcriptional profiles, none of the hydrogenases were upregulated to compensate for the loss of the others. However, the transcript levels of the F_(420) dehydrogenase operon were significantly higher in all strains lacking frh, suggesting a mechanism to sense the redox state of F_(420). The roles of the hydrogenases in energy conservation during growth with each methanogenic pathway are discussed

Morphometrics and distribution of antennal sensillae of both sexes of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Fall Armyworm, Spodoptera frugiperda, is a devastating invasive pest persisting as a menace hampering the progress of Indian agriculture. The morphometrics and distribution of antennal sensilla of both sexes of S. frugiperda were investigated in the study. The antenna was filiform for both sexes and was composed of scape, pedicel and flagellum. Male antennas had more subsegments (65-71) in the flagellum than female (56-70) antennas. Male and female S. frugiperda antennae possessed eight types of sensilla: sensilla trichoidea, sensilla chaetica, sensilla coeloconica, sensilla styloconica, sensilla basiconica, sensilla auricillica, sensilla squamiformia and Bӧhm’s bristles. Sensilla trichoidea was the most abundant sensilla found in the antenna of both sexes and was more abundant in males than in females. It was also noticed that male antenna was longer than the female antenna. The results of the present study helps to assess alternative management strategies with an electrophysiological response of the pest towards sex pheromones and in combination with plant info chemicals for monitoring and management of S. frugiperda in agricultural ecosystems.

Physical conditions in the ISM towards HD185418

We have developed a complete model of the hydrogen molecule as part of the spectral simulation code Cloudy. Our goal is to apply this to spectra of high-redshift star-forming regions where H2 absorption is seen, but where few other details are known, to understand its implication for star formation. The microphysics of H2 is intricate, and it is important to validate these numerical simulations in better-understood environments. This paper studies a well-defined line-of-sight through the Galactic interstellar medium (ISM) as a test of the microphysics and methods we use. We present a self-consistent calculation of the observed absorption-line spectrum to derive the physical conditions in the ISM towards HD185418, a line-of-sight with many observables. We deduce density, temperature, local radiation field, cosmic ray ionization rate, chemical composition and compare these conclusions with conditions deduced from analytical calculations. We find a higher density, similar abundances, and require a cosmic ray flux enhanced over the Galactic background value, consistent with enhancements predicted by MHD simulations.Comment: 31 pages, accepted for publication in Ap

Effect of Inhomogeneous Distribution of Alloying Elements on Integrity of Al-2.1 wt.% Mg Alloy Tubes and Welds

Al-2.1 wt.% Mg alloy is an important nuclear research reactor material. The tubular products of the alloy are usually made by port-hole die extrusion process and several quality control steps are involved during fabrication to assess the quality of the weld joint. The paper describe two cases of  failures of the alloy during fabrication. In one case, a thin wall tube failed during hydro-test at the weld-line while in another, a through-wall crack is observed at the heat affected zone (HAZ) of the weld joint between the thin tube and the tie-plate. In both the cases, the fracture surfaces have the appearance of brittle failure without any gross plastic deformation. Visual inspection, liquid penetrant testing, optical microscopy (OM), scanning electron microscopy (SEM) with energy dispersive spectrometry (EDS), Electron back scattered diffraction (EBSD) and micro-hardness measurement have been carried out for root cause analysis of the failures. EDS analysis at low KV indicates high Magnesium (Mg) and Silicon (Si) content at the fracture surface in both the cases. In the first case, segregation of these alloying elements at the weld-line in the port hole extruded tube has been observed. In the second case, the microstructure at the HAZ location shows grain boundary precipitation of low melting  phase containing Mg, Si and Fe. Presence of Mg and Si reduce the solidus temperature of the grain boundary phase, which is responsible for HAZ liquation leading to failure under tensile stress during cooling

Genetic, Biochemical, and Molecular Characterization of Methanosarcina barkeri Mutants Lacking Three Distinct Classes of Hydrogenase

The methanogenic archaeon Methanosarcina barkeri encodes three distinct types of hydrogenase, whose functions vary depending on the growth substrate. These include the F_(420)-dependent (Frh), methanophenazine-dependent (Vht), and ferredoxin-dependent (Ech) hydrogenases. To investigate their physiological roles, we characterized a series of mutants lacking each hydrogenase in various combinations. Mutants lacking Frh, Vht, or Ech in any combination failed to grow on H_2-CO_2, whereas only Vht and Ech were essential for growth on acetate. In contrast, a mutant lacking all three grew on methanol with a final growth yield similar to that of the wild type and produced methane and CO2 in the expected 3:1 ratio but had a ca. 33% lower growth rate. Thus, hydrogenases play a significant, but nonessential, role during growth on this substrate. As previously observed, mutants lacking Ech failed to grow on methanol-H_2 unless they were supplemented with biosynthetic precursors. Interestingly, this phenotype was abolished in the Δech Δfrh and Δech Δfrh Δvht mutants, consistent with the idea that hydrogenases inhibit methanol oxidation in the presence of H_2, which prevents production of the reducing equivalents needed for biosynthesis. Quantification of the methane and CO_2 produced from methanol by resting cell suspensions of various mutants supported this conclusion. On the basis of the global transcriptional profiles, none of the hydrogenases were upregulated to compensate for the loss of the others. However, the transcript levels of the F_(420) dehydrogenase operon were significantly higher in all strains lacking frh, suggesting a mechanism to sense the redox state of F_(420). The roles of the hydrogenases in energy conservation during growth with each methanogenic pathway are discussed

Energy Conservation via Hydrogen Cycling in the Methanogenic Archaeon Methanosarcina barkeri

Energy conservation via hydrogen cycling, which generates proton motive force by intracellular H_2 production coupled to extracellular consumption, has been controversial since it was first proposed in 1981. It was hypothesized that the methanogenic archaeon Methanosarcina barkeri is capable of energy conservation via H_2 cycling, based on genetic data that suggest that H_2 is a preferred, but nonessential, intermediate in the electron transport chain of this organism. Here, we characterize a series of hydrogenase mutants to provide direct evidence of H_2 cycling. M. barkeri produces H_2 during growth on methanol, a phenotype that is lost upon mutation of the cytoplasmic hydrogenase encoded by frhADGB, although low levels of H_2, attributable to the Ech hydrogenase, accumulate during stationary phase. In contrast, mutations that conditionally inactivate the extracellular Vht hydrogenase are lethal when expression of the vhtGACD operon is repressed. Under these conditions, H_2 accumulates, with concomitant cessation of methane production and subsequent cell lysis, suggesting that the inability to recapture extracellular H_2 is responsible for the lethal phenotype. Consistent with this interpretation, double mutants that lack both Vht and Frh are viable. Thus, when intracellular hydrogen production is abrogated, loss of extracellular H_2 consumption is no longer lethal. The common occurrence of both intracellular and extracellular hydrogenases in anaerobic microorganisms suggests that this unusual mechanism of energy conservation may be widespread in nature

Tumor-Type Agnostic, Targeted Therapies: BRAF Inhibitors Join the Group

In the present review, we briefly discuss the breakthrough advances in precision medicine using a tumor-agnostic approach and focus on BRAF treatment modalities, the mechanisms of resistance and the diagnostic approach in cancers with BRAF mutations. Tumor-type agnostic drug therapies work across cancer types and present a significant novel shift in precision cancer medicine. They are the consequence of carefully designed clinical trials that showed the value of tumor biomarkers, not just in diagnosis but in therapy guidance. Six tumor-agnostic drugs (with seven indications) have been approved through October 2022 by FDA. The first tumor-agnostic treatment modality was pembrolizumab for MSI-H/dMMR solid tumors, approved in 2017. This was followed by approvals of larotrectinib and entrectinib for cancers with NTRK fusions without a known acquired resistance mutation. In 2020, pembrolizumab was approved for all TMB-high solid cancers, while a PD-L1 inhibitor dostarlimab-gxly was approved for dMMR solid cancers in 2021. A combination of BRAF/MEK inhibitors (dabrafenib/trametinib) was approved as a tumor-agnostic therapy in June 2022 for all histologic types of solid metastatic cancers harboring BRAFV600E mutations. In September 2022, RET inhibitor selpercatinib was approved for solid cancers with RET gene fusions. CONCLUSION: Precision cancer medicine has substantially improved cancer diagnostics and treatment. Tissue type-agnostic drug therapies present a novel shift in precision cancer medicine. This approach rapidly expands to provide treatments for patients with different cancers harboring the same molecular alteration

Fission widths of hot nuclei from Langevin dynamics

Fission dynamics of excited nuclei is studied in the framework of Langevin equation. The one body wall-and-window friction is used as the dissipative force in the Langevin equation. In addition to the usual wall formula friction, the chaos weighted wall formula developed earlier to account for nonintegrability of single-particle motion within the nuclear volume is also considered here. The fission rate calculated with the chaos weighted wall formula is found to be faster by about a factor of two than that obtained with the usual wall friction. The systematic dependence of fission width on temperature and spin of the fissioning nucleus is investigated and a simple parametric form of fission width is obtained.Comment: RevTex, 12 pages including 9 Postscript figure