Characterisation of faecal protease activity in irritable bowel syndrome with diarrhoea: origin and effect of gut transit

OBJECTIVES: Faecal serine proteases (FSPs) may play a role in irritable bowel syndrome with diarrhoea (IBS-D), but their origin is unclear. We aimed to structurally characterise them and define the impact of colonic cleansing and transit time. DESIGN: Faecal samples were obtained from 30 healthy volunteers (HV) and 79 patients with IBS-D participating in a trial of ondansetron versus placebo. Colonic transit was measured using radio-opaque markers. Samples were also obtained from 24 HV before and after colonic cleansing with the osmotic laxative MoviPrep. FSPs were purified from faecal extracts using benzamidine-Sepharose affinity chromatography. SDS-PAGE profiled components were identified using trypsinolysis and tandem mass spectrometry. Functional protease activity in faecal extracts was measured using a colorimetric assay based on the proteolysis of azo-casein. RESULTS: Protein analysis identified the most abundant FSPs as being of human origin and probably derived from pancreatic juice. Functional assays showed increased faecal protease (FP) and amylase in patients with IBS-D compared with HV. Those with higher amylase had significantly higher FP and greater anxiety. FP activity correlated negatively with whole gut transit in patients with IBS-D (Spearman r=−0.32, p=0.005) and HV (r=−0.55, p=0.014). Colon cleansing caused a significant rise in FP activity in HV from a baseline of median (IQR) 253 (140–426) to 1031 (435–2296), levels similar to those seen in patients with IBS-D. FSP activity correlated positively with days/week with urgency. CONCLUSIONS: The most abundant FSPs are of human origin. Rapid transit through the colon and/or decreased (possibly bacterial) proteolytic degradation increases their faecal concentration and could contribute to visceral hypersensitivity in patients with IBS-D. CLINICALTRIALS.GOV: NCT00745004

The Epidemiology of Mountain Bike Park Injuries at the Whistler Bike Park, British Columbia (BC), Canada

Objective.—To describe the epidemiology of injuries sustained during the 2009 season at Whistler Mountain Bike Park. Methods.—A retrospective chart review was performed of injured bike park cyclists presenting to the Whistler Health Clinic between May 16 and October 12, 2009. Results.—Of 898 cases, 86% were male (median age, 26 years), 68.7% were Canadian, 19.4% required transport by the Whistler Bike Patrol, and 8.4% arrived by emergency medical services. Identification of 1759 specific injury diagnoses was made, including 420 fractures in 382 patients (42.5%). Upper extremity fractures predominated (75.4%), 11.2% had a traumatic brain injury, and 8.5% were transferred to a higher level of care: 7 by helicopter, 62 by ground, and 5 by personal vehicle. Two patients refused transfer. Conclusions.—Mountain bikers incurred many injuries with significant morbidity while riding in the Whistler Mountain Bike Park in 2009. Although exposure information is unavailable, these findings demonstrate serious risks associated with this sport and highlight the need for continued research into appropriate safety equipment and risk avoidance measures.Medicine, Faculty ofNon UBCEmergency Medicine, Department ofReviewedFacultyOthe