The morphology and exine ornamentation of fresh pollen from four dipterocarp species in Sabah

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The natural history, non-invasive sampling, activity patterns and population genetic structure of the Bornean banteng Bos javanicus lowi in Sabah, Malaysian Borneo

The banteng (Bos javanicus lowi) is an endangered wild bovid that is endemic to the island of Borneo. Within their last stronghold, the Malaysian state of Sabah, their population is believed to be less than 500 individuals, which are threatened with extinction by habitat loss and hunting. The banteng is highly elusive and rarely seen, and their preference for dense and remote tropical forest habitat makes them a highly challenging species to study. No extensive quantitative surveys have been undertaken in Sabah, and there is little information available to underpin their conservation and management. This thesis provides the first baseline data on the Bornean banteng in Sabah using ecological and molecular techniques. In Chapter 2, I created the first extensive natural history account of the banteng, which will help further the knowledge of this species. This compilation helped identify gaps in the knowledge, which were then addressed by this thesis. In Chapter 3, I test non-invasive survey techniques and individual identification, and estimate the population size in two forest reserves. In Chapter 4, I demonstrate that logged forests undergo dramatic changes in structure and ambient temperature, and that banteng mitigate these changes by altering their behaviour to avoid thermal-stress. Chapter 5 presents new information of the population genetic structure of banteng in four forest reserves in Sabah. Using mitochondrial markers I show that the ancestral lineage of the Bornean banteng reinforces the suggestion that they should be recognised as a separate subspecies to the Burmese and Javan banteng. I also show that the banteng experienced a population expansion following their colonisation of Borneo, and that the present genetic diversity indicates the population may be managed as two geographically-distinct units. Chapter 6 summarises the main findings of this thesis and the implications for the conservation of the Bornean banteng in Sabah

The influence of logging upon the foraging behaviour and diet of the endangered Bornean banteng

Logging causes soil compaction and alters the vegetation structure and plant diversity, and ungulates must adapt to modified conditions if they are to survive. We investigated the impact of logging upon the foraging ecology of the Bornean banteng using camera traps and botanical surveys. General linear models were used to explore the effects of site characteristics and plant diversity upon foraging duration, and plant specimens were identified to confirm dietary preferences. Foraging events were recorded over 40,168 nights in five forests. Foraging duration significantly decreased in open areas depauperate in species richness (F=7.82, p=<0.01); however, it increased with elevation (F=3.46, p=0.05). Their diet comprised eight invasive species (Mikania cordata and Chromolaena odorata, Cyperus difformis, Fimbristylis littoralis and Scleria sp., Desmodium triflorum, Eleusine indica and Selaginella sp.), which are characteristic of disturbed forest and fast to establish. Logging creates ideal conditions for these plants, which are preferentially selected by bantengs. Whilst logging may increase forage, the disturbances logging causes to the bantengs and the ensuing poaching may counteract any positive benefits. Developing forest management strategies that incorporate conservation of the bantengs may in turn encourage more favourable conditions for emergent timber species and also conserve this endangered wild cattle species

Herd demography, sexual segregation and the effects of forest management on Bornean banteng Bos javanicus lowi in Sabah, Malaystian Borneo

Between 1973 and 2010, 39.5% of Sabah’s (Malaysian Borneo) natural forest cover was lost to deforestation and conversion to agriculture, therefore the remaining population of endangered Bornean banteng Bos javanicus lowi is being driven towards extinction. The Bornean banteng’s herd demography, sexual segregation and the effects of forest management were investigated at 393 camera locations in 6 forest reserves using generalised estimating equations (GEE) fitted via a generalised linear model (GLM). A total of 43344 camera trap nights and 832 independent banteng events were captured at 93 locations. The identification of 183 bantengs included 22 herds (>1 individual) and 12 solitary bulls, with a herd size range of 2 to 21. Significantly larger herds were observed in forest with <8 yr of post-logging regeneration (PLR), whereas herds were smaller in forest with <3, 4 and 16 yr of PLR. Within these forests, herds were significantly larger along logging roads than in open sites and on forest trails. Herds were significantly larger in upland compared to lowland dipterocarp forest, but significantly smaller when closer to the forest border. Bachelor herds were observed as frequently as mixed-sex herds, and there was a significantly higher capture frequency of female herds in the dry season, supporting the theory of sexual segregation. Frequency of calf births was highest in March and September, and calf captures peaked in June and July. This study contributes to a better understanding of banteng ecology, and will assist in the production of effective management strategies aimed at providing suitable habitat for re-population and enabling banteng population persistence

Spatial and temporal behavioural responses of wild cattle to tropical forest degradation

<div><p>Identifying the consequences of tropical forest degradation is essential to mitigate its effects upon forest fauna. Large forest-dwelling mammals are often highly sensitive to environmental perturbation through processes such as fragmentation, simplification of habitat structure, and abiotic changes including increased temperatures where the canopy is cleared. Whilst previous work has focused upon species richness and rarity in logged forest, few look at spatial and temporal behavioural responses to forest degradation. Using camera traps, we explored the relationships between diel activity, behavioural expression, habitat use and ambient temperature to understand how the wild free-ranging Bornean banteng (<i>Bos javanicus lowi</i>) respond to logging and regeneration. Three secondary forests in Sabah, Malaysian Borneo were studied, varying in the time since last logging (6–23 years). A combination of generalised linear mixed models and generalised linear models were constructed using >36,000 trap-nights. Temperature had no significant effect on activity, however it varied markedly between forests, with the period of intense heat shortening as forest regeneration increased over the years. Bantengs regulated activity, with a reduction during the wet season in the most degraded forest (z = -2.6, Std. Error = 0.13, p = 0.01), and reductions during midday hours in forest with limited regeneration, however after >20 years of regrowth, activity was more consistent throughout the day. Foraging and use of open canopy areas dominated the activity budget when regeneration was limited. As regeneration advanced, this was replaced by greater investment in travelling and using a closed canopy. Forest degradation modifies the ambient temperature, and positively influences flooding and habitat availability during the wet season. Retention of a mosaic of mature forest patches within commercial forests could minimise these effects and also provide refuge, which is key to heat dissipation and the prevention of thermal stress, whilst retention of degraded forest could provide forage.</p></div

Body condition scoring of Bornean banteng in logged forests

© The Author(s) 2016. Background The Bornean banteng (Bos javanicus lowi) is an endangered subspecies that often inhabits logged forest; however very little is known about the effects of logging on their ecology, despite the differing effects this has on other ungulate species. A body condition scoring system was created for the Bornean banteng using camera trap photographs from five forests in Sabah, Malaysia, with various past and present management combinations to establish if banteng nutrition suffered as a result of forest disturbance. Results One hundred and eleven individuals were photographed over 38,009 camera trap nights from April 2011 to June 2014 in five forests. Banteng within forests that had a recent history of reduced-impact logging had higher body condition scores than banteng within conventionally logged forest. Conversely, when past logging was conducted using a conventional technique and the period of forest regeneration was relatively long; the banteng had higher body condition scores. Conclusion The body condition scoring system is appropriate for monitoring the long-term nutrition of the Bornean banteng and for evaluating the extent of the impact caused by present-day reduced-impact logging methods. Reduced-impact logging techniques give rise to individuals with the higher body condition scores in the shorter term, which then decline over time. In contrast the trend is opposite for conventional logging, which demonstrates the complex effects of logging on banteng body condition scores. This is likely to be due to differences in regeneration between forests that have been previously logged using differing methods

The foot-health of people with diabetes in regional and rural Australia:Baseline results from an observational cohort study

Background: There is limited Australian epidemiological research that reports on the foot-health characteristics ofpeople with diabetes, especially within rural and regional settings. The objective of this study was to explore theassociations between demographic, socio-economic and diabetes-related variables with diabetes-related footmorbidity in people residing in regional and rural Australia.Methods: Adults with diabetes were recruited from non-metropolitan Australian publicly-funded podiatry services. Theprimary variable of interest was the University of Texas diabetic foot risk classification designated to each participant atbaseline. Independent risk factors for diabetes-related foot morbidity were identified using multivariable analysis.Results: Eight-hundred and ninety-nine participants enrolled, 443 (49.3%) in Tasmania and 456 (50.7%) in Victoria.Mean age was 67 years (SD 12.7), 9.2% had type 1 diabetes, 506 (56.3%) were male, 498 (55.4%) had diabetes for longerthan 10 years and 550 (61.2%) either did not know the ideal HbA1c target or reported that it was ≥7.0. A majority hadperipheral neuropathy or worse foot morbidity (61.0%). Foot morbidity was associated with male sex (OR 2.42, 95% CI1.82–3.22), duration of diabetes > 20 years (OR 3.25, 95% CI 2.22–4.75), and Tasmanian residence (OR 3.38, 95% CI 2.35–4.86).Conclusions: A high proportion of the regional Australian clinical population with diabetes seen by the publiclyfunded podiatric services in this study were at high risk of future limb threatening foot morbidity, and participantsresiding in Northern Tasmania are more likely to have worse diabetes-related foot morbidity than those from regionalVictoria. Service models should be reviewed to ensure that diabetes-related foot services are appropriately developedand resourced to deliver interdisciplinary evidence-based care

Hunting pressure a key contributor to the impending extinction of Bornean wild cattle

Widespread and unregulated hunting of ungulates in Southeast Asia is resulting in population declines and localised extinctions. Increased access to previously remote tropical forest following logging and changes in land-use facilitates hunting of elusive wild cattle in Borneo, which preferentially select secluded habitat. We collated the first population parameters for the Endangered Bornean banteng Bos javanicus lowi and developed population models to simulate the effect of different hunting offtake rates on survival and the recovery of the population using reintroduced captive-bred individuals. Our findings suggest that the banteng population in Sabah is geographically divided into 4 management units based on connectivity: the Northeast, Sipitang (West), Central and Southeast, which all require active management to prevent further population decline and local extinction. With only 1% offtake, population growth ceased in the Northeast and Sipitang. In the Southeast and Central units, growth ceased at 2 and 4% offtake, respectively. Extinction was estimated at 21-39 yr when offtake was 5%, occurring first in Sipitang and last in the Central unit. Supplementing the population with captive-bred individuals suggested that inbreeding was likely to limit population growth if using ≤20 founder individuals. Translocating 2 individuals for a 10 yr period, starting after 20 yr of captive breeding resulted in a faster population recovery over 100 yr and a lower extinction probability. Our results suggest that shielding the population against further losses from hunting will be key to their survival in the wild, provided that active management in the form of captive breeding is developed in the interim

Using natural marks in a spatially explicit capture-recapture framework to estimate preliminary population density of cryptic endangered wild cattle in Borneo

The behaviour of cryptic tropical forest ungulates that are not identifiable from unique coat colour and patterns often impedes detectability and investigations of population density, which underpin conservation plans. The shy and endangered Bornean banteng has a declining trend, but quantifying this requires sufficient detections to estimate robust population parameters, which are currently unavailable. Using intensive camera trapping and individual identification from natural marks by two observers, we estimated the baseline population density of Bornean bantengs in Malua and Tabin forests in Sabah (Malaysian Borneo) using a spatially explicit capture-recapture framework. We also investigated the efficacy of two commonly-used survey methods (camera trapping and signs) that have previously failed to detect the species, by contrasting capture frequencies to estimate the probability of odds of capture. Density estimates and simulated 95% confidence limits were exceptionally low in both forests and with negligible differences arising from small disparities in the interpretation of natural marks. Density in Malua ranged from 0.5 individuals per 100 km2 (0.21–1.48) to 0.56 (0.15–2.09), and in Tabin between 0.61 (0.32–1.16) to 0.95 (0.54–1.66). The capture odds were significantly greater for camera traps (X2 = 20, p  4, p < 0.001); sign survey efficacy declined at higher elevations and under dense canopy. Using natural marks for individual identification was resource-demanding, but provided robust population density parameters for an otherwise challenging species to detect. Extremely low-density estimates of Bornean bantengs highlights the urgency for greater control of poaching, which is almost certainly decimating the population. Rapid implementation of actions to mitigate against further losses are essential for halting the declining trend. The estimation of density parameters in other forests in Sabah that contain bantengs would set the context for our density estimates. It would additionally provide a basis for long-term population monitoring, and facilitate investigations into the effectiveness of enforcement strategies

Trends and risk factors for human Q fever in Australia, 1991-2014

Australian abattoir workers, farmers, veterinarians and people handling animal birthing products or slaughtering animals continue to be at high risk of Q fever despite an effective vaccine being available. National Notifiable Diseases Surveillance System data were analysed for the period 1991–2014, along with enhanced risk factor data from notified cases in the states of New South Wales and Queensland, to examine changes in the epidemiology of Q fever in Australia. The national Q fever notification rate reduced by 20% [incident rate ratio (IRR) 0·82] following the end of the National Q fever Management Program in 2006, and has increased since 2009 (IRR 1·01–1·34). Highest rates were in males aged 40–59 years (5·9/100 000) and 87% of Q fever cases occurred in New South Wales and Queensland. The age of Q fever cases and proportion of females increased over the study period. Based on the enhanced risk factor data, the most frequently listed occupation for Q fever cases involved contact with livestock, followed by ‘no known risk’ occupations. More complete and comparable enhanced risk factor data, at the State/Territory and national levels, would aid in further understanding of the epidemiology of Q fever