Genotoxicity evaluation of individual cigarette smoke toxicants using the in vitro γH2AX assay by High Content Screening

AbstractCigarette smoke is a complex mixture consisting of more than 5600 identified chemical constituents of which approximately 150 have been identified so far as “tobacco smoke toxicants”. Proposals made by the World Health Organisation Framework Convention on Tobacco Control mandate the lowering of nine tobacco smoke priority toxicants, including 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), and benzo[a]pyrene (B[a]P) and monitoring the levels of a further nine including cadmium.Here, we evaluated the genotoxic potential in human bronchial epithelial BEAS-2B cells of four cigarette smoke toxicants; NNK, NNN, B[a]P and cadmium using the novel in vitro γH2AX assay by High Content Screening (HCS). We also examined the genotoxicity of binary mixtures of NNK and NNN reporting their relative contribution to the genotoxic end-point.The results of this preliminary assessment showed that the in vitro γH2AX assay by HCS could be used as a pre-screening tool to detect and quantify the genotoxicity effect of cigarette smoke toxicants individually and in binary mixture. Moreover, the data produced could contribute to the prioritisation of toxicant reduction research in modified tobacco products

Isoscalar off-shell effects in threshold pion production from pd collisions

We test the presence of pion-nucleon isoscalar off-shell effects in the $pd\to \pi^+ t$ reaction around the threshold region. We find that these effects significantly modify the production cross section and that they may provide the missing strength needed to reproduce the data at threshold.Comment: 6 pages, REVTeX, twocolumn, including 3 figures (Postscript), uses psfig, updated and extended versio

High-level expression of Pinus sylvestris glutamine synthetase in Escherichia coli Production of polyclonal antibodies against the recombinant protein and expression studies in pine seedlings

AbstractIn a previous work we reported the molecular characterization of a glutamine synthetase (GS; EC 6.3.1.2.) complementary DNA from a woody plant (Cantón et al. (1993) Plant Mol. Biol. 22, 819–828). The isolated cDNA (pGSP114) encoding a Scots pine (Pinus sylvestris) cytosolic subunit, has been subcloned into the expression vector pET3c to overproduce the GS polypeptide in Escherichia coli cells. The recombinant GS protein showed the same molecular size as a native Scots pine GS subunit. Antibodies against the pET3c-GSP114 encoded protein were raised in rabbits by injecting purified preparations and specificity was determined by immunoprecipitation of GS activity present in pine crude extracts. In spite of the antibodies were able to recognize both cytosolic and chloroplastic GS in tomato plants, they were unable to immunodetect chloroplastic GS in green cotyledons of pine seedlings and cytosolic GS was the unique recognized polypeptide. Unlike to that found in other plant species, cytosolic GS was strongly expressed in green tissues as determined by protein and Northern analysis. Our results suggest a key role for cytosolic GS in photosynthetic tissues of conifers

Epidemiology and echinocandin susceptibility of Candida parapsilosis sensu lato species isolated from bloodstream infections at a Spanish University Hospital

Objectives: The aims of this work were to study the epidemiological profiles, differences in echinocandin susceptibilities and clinical relevance of the Candida parapsilosis sensu lato species isolated from proven fungaemia cases at La Fe University Hospital of Valencia (Spain) from 1995 to 2007.The aims of this work were to study the epidemiological profiles, differences in echinocandin susceptibilities and clinical relevance of the Candida parapsilosis sensu lato species isolated from proven fungaemia cases at La Fe University Hospital of Valencia (Spain) from 1995 to 2007. Results: The prevalence of these species was: C. parapsilosis sensu stricto, 74.4%; Candida orthopsilosis, 23.54%; and Candida metapsilosis, 2.05%. The incidence of the species complex as agents of fungaemia remained stationary until 2005 and doubled in 2006. The incidence of C. orthopsilosis showed an increasing trend during the study period, while C. parapsilosis sensu stricto incidence diminished. Also, an important epidemiological change was observed starting in 2004, when 86.5% of the C. parapsilosis sensu lato strains were found in adult patients, while before that year only 13.5% of the isolates were found in this population. Conclusions: Echinocandin drug susceptibility testing using the CLSI M27-A3 document showed a wide range of MIC values (0.015?4 mg/L), with micafungin being the most potent in vitro inhibitor followed by anidulafungin and caspofungin (MIC geometric mean of 0.68, 0.74 and 0.87 mg/L, respectively). C. metapsilosis was the most susceptible species of the complex to anidulafungin and micafungin in vitro (MIC50 for anidulafungin and micafungin: 0.06 mg/L), while there were no differences between C. parapsilosis sensu lato species when caspofungin MIC50s were compared (MIC50 1.00 mg/L). Differences in caspofungin in vitro susceptibility were observed between the different clinical service departments of La Fe Hospital.Echinocandin drug susceptibility testing using the CLSI M27-A3 document showed a wide range of MIC values (0.015?4 mg/L), with micafungin being the most potent in vitro inhibitor followed by anidulafungin and caspofungin (MIC geometric mean of 0.68, 0.74 and 0.87 mg/L, respectively). C. metapsilosis was the most susceptible species of the complex to anidulafungin and micafungin in vitro (MIC50 for anidulafungin and micafungin: 0.06 mg/L), while there were no differences between C. parapsilosis sensu lato species when caspofungin MIC50s were compared (MIC50 1.00 mg/L). Differences in caspofungin in vitro susceptibility were observed between the different clinical service departments of La Fe Hospital.Fil: Garcia, Guillermo Manuel. Public Health Research Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Canton, Emilia. Hospital Universitario la Fe; EspañaFil: Pemán, Javier. Hospital Universitario la Fe; EspañaFil: Dilger, Amanda. Public Health Research Institute; Estados UnidosFil: Romá, Eva. Hospital Universitario la Fe; EspañaFil: Perlin, David S.. Public Health Research Institute; Estados Unido

A Wearable Textile 3D Gesture Recognition Sensor Based on Screen-Printing Technology

[EN] Research has developed various solutions in order for computers to recognize hand gestures in the context of human machine interface (HMI). The design of a successful hand gesture recognition system must address functionality and usability. The gesture recognition market has evolved from touchpads to touchless sensors, which do not need direct contact. Their application in textiles ranges from the field of medical environments to smart home applications and the automotive industry. In this paper, a textile capacitive touchless sensor has been developed by using screen-printing technology. Two different designs were developed to obtain the best configuration, obtaining good results in both cases. Finally, as a real application, a complete solution of the sensor with wireless communications is presented to be used as an interface for a mobile phone.The work presented is funded by the Conselleria d'Economia Sostenible, Sectors Productius i Treball, through IVACE (Instituto Valenciano de Competitividad Empresarial) and cofounded by ERDF funding from the EU. Application No.: IMAMCI/2019/1. This work was also supported by the Spanish Government/FEDER funds (RTI2018-100910-B-C43) (MINECO/FEDER).Ferri Pascual, J.; Llinares Llopis, R.; Moreno Canton, J.; Ibáñez Civera, FJ.; Garcia-Breijo, E. (2019). A Wearable Textile 3D Gesture Recognition Sensor Based on Screen-Printing Technology. Sensors. 19(23):1-32. https://doi.org/10.3390/s19235068S1321923Chakraborty, B. K., Sarma, D., Bhuyan, M. K., & MacDorman, K. F. (2017). Review of constraints on vision‐based gesture recognition for human–computer interaction. IET Computer Vision, 12(1), 3-15. doi:10.1049/iet-cvi.2017.0052Zhang, Z. (2012). Microsoft Kinect Sensor and Its Effect. IEEE Multimedia, 19(2), 4-10. doi:10.1109/mmul.2012.24Rautaray, S. S. (2012). Real Time Hand Gesture Recognition System for Dynamic Applications. International Journal of UbiComp, 3(1), 21-31. doi:10.5121/iju.2012.3103Karim, R. A., Zakaria, N. F., Zulkifley, M. A., Mustafa, M. M., Sagap, I., & Md Latar, N. H. (2013). Telepointer technology in telemedicine : a review. BioMedical Engineering OnLine, 12(1), 21. doi:10.1186/1475-925x-12-21Santos, L., Carbonaro, N., Tognetti, A., González, J., de la Fuente, E., Fraile, J., & Pérez-Turiel, J. (2018). Dynamic Gesture Recognition Using a Smart Glove in Hand-Assisted Laparoscopic Surgery. Technologies, 6(1), 8. doi:10.3390/technologies6010008Singh, A., Buonassisi, J., & Jain, S. (2014). Autonomous Multiple Gesture Recognition System for Disabled People. International Journal of Image, Graphics and Signal Processing, 6(2), 39-45. doi:10.5815/ijigsp.2014.02.05Ohn-Bar, E., & Trivedi, M. M. (2014). Hand Gesture Recognition in Real Time for Automotive Interfaces: A Multimodal Vision-Based Approach and Evaluations. IEEE Transactions on Intelligent Transportation Systems, 15(6), 2368-2377. doi:10.1109/tits.2014.2337331Khan, S. A., & Engelbrecht, A. P. (2010). A fuzzy particle swarm optimization algorithm for computer communication network topology design. Applied Intelligence, 36(1), 161-177. doi:10.1007/s10489-010-0251-2Abraham, L., Urru, A., Normani, N., Wilk, M., Walsh, M., & O’Flynn, B. (2018). Hand Tracking and Gesture Recognition Using Lensless Smart Sensors. Sensors, 18(9), 2834. doi:10.3390/s18092834Zeng, Q., Kuang, Z., Wu, S., & Yang, J. (2019). A Method of Ultrasonic Finger Gesture Recognition Based on the Micro-Doppler Effect. Applied Sciences, 9(11), 2314. doi:10.3390/app9112314Lien, J., Gillian, N., Karagozler, M. E., Amihood, P., Schwesig, C., Olson, E., … Poupyrev, I. (2016). Soli. ACM Transactions on Graphics, 35(4), 1-19. doi:10.1145/2897824.2925953Sang, Y., Shi, L., & Liu, Y. (2018). Micro Hand Gesture Recognition System Using Ultrasonic Active Sensing. IEEE Access, 6, 49339-49347. doi:10.1109/access.2018.2868268Ferri, J., Lidón-Roger, J., Moreno, J., Martinez, G., & Garcia-Breijo, E. (2017). A Wearable Textile 2D Touchpad Sensor Based on Screen-Printing Technology. Materials, 10(12), 1450. doi:10.3390/ma10121450Nunes, J., Castro, N., Gonçalves, S., Pereira, N., Correia, V., & Lanceros-Mendez, S. (2017). Marked Object Recognition Multitouch Screen Printed Touchpad for Interactive Applications. Sensors, 17(12), 2786. doi:10.3390/s17122786Ferri, J., Perez Fuster, C., Llinares Llopis, R., Moreno, J., & Garcia‑Breijo, E. (2018). Integration of a 2D Touch Sensor with an Electroluminescent Display by Using a Screen-Printing Technology on Textile Substrate. Sensors, 18(10), 3313. doi:10.3390/s18103313Cronin, S., & Doherty, G. (2018). Touchless computer interfaces in hospitals: A review. Health Informatics Journal, 25(4), 1325-1342. doi:10.1177/1460458217748342Haslinger, L., Wasserthal, S., & Zagar, B. G. (2017). P3.1 - A capacitive measurement system for gesture regocnition. Proceedings Sensor 2017. doi:10.5162/sensor2017/p3.1Cherenack, K., & van Pieterson, L. (2012). Smart textiles: Challenges and opportunities. Journal of Applied Physics, 112(9), 091301. doi:10.1063/1.474272

Toros o semen

Otro componente fundamental para tratar de completar la Información sanitaria y reproductiva de un rodeo bovino ya sea para carne o leche son los toros o el semen empleado para IA. En ese sentido, es importante también averiguar el historial sanitario del rodeo, en lo que respecta las enfermedades de transmisión sexual (tricomonosis y campilobacteriosis, principalmente), así como otros aspectos de manejo que pudieran haber influido para que un toro no se hubiese comportado adecuadamente durante el servicio: condición corporal previa, alteraciones podales o articulares, patologías de tracto genital (orquitis, epididimitis, etc.), entre otras.EEA BalcarceFil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina.Fil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina.Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina

Pathology of cattle experimentally intoxicated with ground Ricinus communis seeds

Five Aberdeen Angus calves were inoculated intra-ruminally with ground seeds of Ricinus communis at doses of 1, 1.5, 2 or 3 gr per kg of body weight, or with saline solution (control), respectively. Grossly, all intoxicated animals showed hemorrhages in abdominal serosas, epicardium, endocardium, spleen, pre-stomachs, abomasum, and small and large intestine, and diffuse edema of the ruminal mucosa. Microscopically, in all animals inoculated with R. communis seeds, the main feature was the presence of pyknotic and karyorrhectic nuclei in the endothelium of central nervous system, hepatic, ruminal, intestinal, glomerular and alveolar capillaries, and in lymphoid cells of multiple organs. Apoptosis, confirmed by activated caspase-3 immunohistochemistry, was observed in these cells. No gross or microscopic lesions were observed in the control animal. The results of this study suggest that apoptosis is the main mechanism of cell death in cattle intoxicated with R. communis seeds.EEA BalcarceFil: Marin, Raúl. Universidad Nacional de Jujuy; Argentina.Fil: Schild, Carlos. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay.Fil: García, Juan. Universidad de la República. Centro Universitario de la Región del Este; Uruguay.Fil: Cantón, Germán. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Micheloud, Juan. INTA, Estación Experimental Agropecuaria Salta; Argentina.Fil: Morrell, Eleonora. INTA, Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Uzal, Francisco. Universidad de California; Estados Unidos

Retrospective analysis of cattle poisoning in Argentina (2000-2013)

A retrospective analysis (2000 to 2013) of cattle poisoning caused by toxic plants and other compounds was carried out in the Pampas region of Argentina by the Animal Health Group of INTA-EEA, Balcarce. During this period, 1263 reports of diseases of different etiologies (infectious, parasitic, toxic, metabolic and miscellaneous) were recorded in cattle, by collecting anamnestic, clinical and pathological information. A toxic etiology was diagnosed in 21.1% of these reports. Iatrogenic poisoning caused by ionophores was the most frequently recorded etiology. Consumption of toxic plants (Wedelia glauca, Solanum glaucophyllum, among others), mycotoxins (Claviceps purpurea, Claviceps paspali, Epichloë coenophiala, among others), and plants producing cyanide and nitrates/nitrites were also commonly diagnosed. The high frequency of toxic episodes and the difficulties in their diagnosis by practitioners in our livestock production systems emphasizes the importance of this report.EEA BalcarceFil: García, Juan Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Canton, German Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Spath, Ernesto Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Odriozola, Ernesto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Garcia, Bernardo L. Actividad Privada; ArgentinaFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentin