Reduced expression of mir15a in the blood of patients with oral squamous cell carcinoma is associated with tumor staging

MicroRNAs (miRNAs) mirl 5a and let7a are iMportant regulators of bcl-2, ras and c-myc proteins Considering that these miRNAs are commonly altered in many human cancers and that these proteins are reported to be altered in oral squamous cell carcinoma (OSCC), we investigated them in a set of OSCC cases \u27I he miRNAs as well as the proteins were evaluated in the tumor and blood of 20 patients by real-time quantitative PCR and iMmunohistochemistry, respectively The expression of nfirl5a and bcl-2 proteins in the tumors was not associated with each other or with tumor staging On the other hand, we found reduced expression of this miRNA in the blood of patients with an advanced stage of OSCC and with lymph node metastasis The expression of let7a in the tumor and blood was not associated with tumor size lymph node metastasis, tumor staging and immunoexpression of ras and c-myc proteins In conclusion, the present study shows that reduced expression of Mir15a is associated with OSCC stagin

Catálogo Taxonômico da Fauna do Brasil: setting the baseline knowledge on the animal diversity in Brazil

The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others

Análise molecular do gene WWOX no carcinoma de células escamosas da cavidade bucal

Exportado OPUSMade available in DSpace on 2019-08-14T03:20:24Z (GMT). No. of bitstreams: 1 disserta__o_mestrado___fl_vio_juliano_garcia_santos_pimenta.pdf: 1042118 bytes, checksum: ae466ec86b10d42866f90d2f73f40374 (MD5) Previous issue date: 15Carcinoma de células escamosas (CCE) é a neoplasia maligna mais comum da cavidade bucal, e representa aproximadamente 90% de todas as neoplasias malignas da boca. Sabe-se que a carcinogênese bucal ocorre como um processo de acumulação de danos genéticos como a ativação de oncogenes, inativação de genes supressores de tumor e perda de heterozigosidade em vários cromossomos. Contudo, um mecanismo molecular específico envolvido no processo de carcinogênese do CCE ainda não foi completamente descrito. O gene WWOX é um supressor de tumor localizado no braço longo do cromossomo 16 (16q23.3-24.1). Esse gene expande a região do segundo mais expresso sítio de fragilidade comum, FRA16D. Alterações desse gene têm sido demonstradas em vários tipos de câncer como o carcinoma de células escamosas de esôfago e pulmão, que apresentam os fatores etiológicos semelhantes aos do CCE da cavidade bucal. Para avaliar o mecanismo do gene WWOX no CCE de boca, nós analisamos 20 tumores primários e 10 casos de mucosa bucal normal. A transcrição do mRNA foi alterada em 35% dos tumores, com ausências completa de transcrição de dois casos (#CA3 e #CA18), ausência dos exons 6-8 (#CA2, #CA5, #CA21 e #CA24), do exon 7 (#CA2) e perda parcial dos exons 8 e 9 (#CA12). Com o objetivo de determinar se os transcritos aberrantes foram traduzidos em proteínas, às amostras foram submetidas ao Western blot. Os transcritos alterados presentes nos tumores #CA2, #CA5, #CA12, #CA21 e #CA24 não foram detectados pelo Western Blot, sugerindo que eles não são traduzidos em proteínas. Análise da expressão da proteína através da imunoistoquímica revelou uma redução da expressão em 40% dos casos, quando comparado com a mucosa normal. Além disso, uma nova mutação somática (S329F) foi encontrada no #CA12. A presença de alterações na transcrição do mRNA foi correlacionado com a redução da expressão da proteína WWOX nos tumores. Os resultados demonstram que 50% dos CCE de boca apresentam alterações no gene WWOX, que poderia contribuir para o processo de carcinogênese do câncer de boca.Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity, representing approximately 90% of all oral carcinomas. It is acknowledged that oral carcinogenesis is a multi-step process of accumulated genetic damage, such as activation of oncogenes, tumor suppressor genes inactivation, and loss of heterozygosity at numerous chromosomal locations. However, the specific molecular mechanisms involved in OSCC tumorigenesis have not yet been completely elucidated. The WWOX gene is a candidate tumor suppressor gene located at 16q23.3-24.1, spanning the second most common fragile site, FRA16D. Alterations of this gene have been demonstrated in multiple types of cancer, including lung and esophageal squamous cell carcinoma, which share some predictive factors with OSCCs. To evaluate the role of the WWOX gene in OSCC, we analyzed 20 tumors and 10 normal oral mucosas. RNA transcription was altered in 35% of tumors, with complete absence of transcripts as well as absence of exons 6-8, exon 7, exons 6-8 and partial loss of exons 8 and 9. To determine if the aberrant transcripts were translated, Western blots were carried out; however only the normal protein was detected. Immunohistochemistry showed a reduction in WWOX protein expression affecting 40% of tumors when compared with normal mucosa. In addition, a novel somatic mutation (S329F) was found. The presence of alterations in mRNA transcription correlated with the reduced expression of WWOX protein in the tumors. These results show that WWOX gene is frequently altered in OSCC and may contribute to the carcinogenesis processes in oral cancer