Nucleotide sequence diversity of HLA class II genes in Australian Aborigines and populations of Asia-Oceania

The aim of this thesis was to investigate nucleotide sequence diversity of HLA class II genes in Australian Aborigines and indigenous peoples of Asia-Oceania. Nineteen study populations represented eight major ethnic groups including Australian Aborigines, Papua New Guinean highlanders, coastal Melanesians, Polynesians, Micronesians, Javanese, southern and northern Chinese, and a minority group from northwestern China. Using PCR-based technologies, the nucleotide sequence polymorphism in exon 2 DRB1, DRB3, DRB5, DQA1 and DQB1 genes was examined in all these populations. The DPB1 exon 2 polymorphism was examined in Australian Aborigines and a Chinese population. Six novel HLA class II alleles including four DRB1, one DRB5 and one DPBl were discovered in this study by the occurrence of unusual hybridization patterns in the PCR-SSO typing procedure and were confirmed by DNA Sequencing. These new alleles, DRB1*0412, 1408, 1409, 1410, DRB5*0203 and DBP1*2201 have been recognized by the WHO Nomenclature Committee. The nucleotide sequences and the deduced amino acid sequences of the novel class II alleles indicated that multiple molecular mechanisms were involved in generating these alleles including point mutation and hypermutational events of segmental transfer and intra-exonic recombination. In two cases (DRB1*0412 and DRB1*1410), hypermutational events have created unique peptide binding sites which are drastically different from all their putative progenitor molecules. Five of the six novel alleles were found in Australian Aborigines and four novel DRB1 alleles were detected in 45% of the Aboriginal individuals tested. PCR-SSO typing revealed some HLA class II polymorphisms previously difficult or impossible to detect with more traditional typing techniques. Remarkable differences in the V class II HLA allele frequency distributions, especially in the subtypes of major DR antigen groups, were observed between the study populations. Australian Aborigines showed the most divergent class II HLA profile; most of their DRB1 alleles did not overlap with other study populations. PNG highlanders and Javanese were highly homogeneous with quite restricted class II HLA distributions. Other Oceanic populations of Polynesians, Micronesians and coastal Melanesians were each characterized with unique class II HLA distribution but shared common features which indicated their historical ties. Distinctive HLA distributions were observed between Chinese populations from southern and northern China, while the minority group from northwestern China demonstrated a mixed ancestry of both Caucasoids and Orientals. Further information came from the analysis of HLA-DR, -DQ haplotypes. A total of 80 three-locus or four-locus DR-DQ combinations including 16 DR2-related, 12 DR4-related, 11 DR5- related, and 24 DR6-related haplotypes were inferred from the study populations. Haplotype frequencies were used to calculate genetic distances between these populations and to reconstruct population phylogeny, which proved a sensitive indicator of population affinities. The unusual linkage relationships detected in the study populations also had important implications for the understanding of MHC evolution. Knowledge of the nucleotide sequence polymorphism of HLA class II genes in general populations has fundamental importance in HLA-related clinical investigations. The apparent lack of susceptible alleles in the HLA gene pool of native Australians and Pacific islanders, or the high frequency of protective alleles, might partly explain the extremely low incidence of autoimmune diseases in these populations

HLA Class I and KIR Genes Do Not Protect Against HIV Type 1 Infection in Highly Exposed Uninfected Individuals With Hemophilia A

A recent genome-wide association study (GWAS) involving patients with hemophilia A who were exposed to but uninfected with human immunodeficiency virus type 1 (HIV-1) did not reveal genetic variants associated with resistance to HIV-1 infection, beyond homozygosity for CCR5-Δ32. Since variation in HLA class I and KIR genes is not well interrogated by standard GWAS techniques, we tested whether these 2 loci were involved in protection from HIV-1 infection in the same hemophilia cohort, using controls from the general population. Our data indicate that HLA class I alleles, presence or absence of KIR genes, and functionally relevant combinations of the HLA/KIR genotypes are not involved in resistance to parenterally transmitted HIV-1 infectio