Research journal of pharmaceutical, biological and chemical sciences

The ongoing pollution of water resources with a variety of lubricating oils, the insufficiently developed methods of purification of natural and waste water poses the problem of finding ways to restore the natural qualities of the environment. The authors see a solution to this problem in the wide use of activity of individual associations of oil-oxidizing microorganisms (OOM) in combination with the plant-derived sorbents (buckwheat, oat, wheat and barley husk), which allows deep controlled oxidation of these contaminants down to CO2 and H2O. It was found that the multi-species OOM communities take more active part in biodegradation of mineral, semi-synthetic and synthetic oils, than those with the limited species composition. The growth, development and activity in the oil biodegradation is determined by the nature of the contamination. The population growth maxima lie between 5 and 14 days, and decrease to 2 to 6 hours under the influence of sorbents

Biodestruction of lubricated motor oils in sewage water with the use of pilot biological installation

This article presents the results of an experiment on the choice of technology for purification and final purification of wastewater of small sewage enterprises and large-scale productions from waste lubricating oils of mineral origin with a wide change in the pH of the medium, providing dissolved oxygen and biogenic elements. It is proved that a high degree of biodegradation of oil-contaminated effluents is achieved by the wide use of the newly created consortium of hydrocarbon-oxidizing microorganisms (HOM), which includes nine strains belonging to the genera: Alcaligenes(1 species), Bacillus (1 species), Brevibacterium (2 species), Clostridium (1 species), Flavobacterium (1 species), Micrococcus (1 species) and Pseudomonas (2 species). The association of this microorganismsis able to oxidize various fractions of oil ranging from n-alkalis, to asphaltenes, including mineral, semi-synthetic and synthetic oils of various origin. It has been established that the load change from 25 to 100 mg/dm3(COD from 450 to 650 mg/dm3) when using lubricating oil in stock ROB almost does not affect the degree of biodegradation of the original pollution. During the contacting of the ON-NOVO consortium with the oil-containing waste liquid in the bioreactor and further in the secondary settling tank, with a total duration of 1.5 to 4.5 hours, the degree of effluent purification reaches up to 77.4%. With the addition of a normalized amount of nutrients in the amount of 20 mg/dm3 (optimal zone), the degree of neutralization of waste water exceeds 86%. In terms of residual content of oxidized mineral oil, the cleaning intensity exceeds 94%.526-53

Biodestruction of lubricated motor oils in sewage water with the use of pilot biological installation

This article presents the results of an experiment on the choice of technology for purification and final purification of wastewater of small sewage enterprises and large-scale productions from waste lubricating oils of mineral origin with a wide change in the pH of the medium, providing dissolved oxygen and biogenic elements. It is proved that a high degree of biodegradation of oil-contaminated effluents is achieved by the wide use of the newly created consortium of hydrocarbon-oxidizing microorganisms (HOM), which includes nine strains belonging to the genera: Alcaligenes(1 species), Bacillus (1 species), Brevibacterium (2 species), Clostridium (1 species), Flavobacterium (1 species), Micrococcus (1 species) and Pseudomonas (2 species). The association of this microorganismsis able to oxidize various fractions of oil ranging from n-alkalis, to asphaltenes, including mineral, semi-synthetic and synthetic oils of various origin. It has been established that the load change from 25 to 100 mg/dm3(COD from 450 to 650 mg/dm3) when using lubricating oil in stock ROB almost does not affect the degree of biodegradation of the original pollution. During the contacting of the ON-NOVO consortium with the oil-containing waste liquid in the bioreactor and further in the secondary settling tank, with a total duration of 1.5 to 4.5 hours, the degree of effluent purification reaches up to 77.4%. With the addition of a normalized amount of nutrients in the amount of 20 mg/dm3 (optimal zone), the degree of neutralization of waste water exceeds 86%. In terms of residual content of oxidized mineral oil, the cleaning intensity exceeds 94%.526-53

The treatment of articular cartilage injuries with mesenchymal stem cells in different animal species

One of the major problems observed in veterinary practice is Articular cartilage injuries in animals. In terms of agriculture, it leads to their culling from the herd, even if they are highly productive animals. With companion animals, owners usually have to decide between euthanasia or long-term sometimes life-long treatment of the injury by a veterinarian. The use of Mesenchymal Stem Cells (MSCs) for the treatment of cartilage injury in veterinary medicine is based on the good results observed in preclinical studies, where large animals have been used as experimental models to study the regenerative activity of MSCs. According to the literature, MSCs in veterinary medicine have been used to treat cartilage injury of dogs and horses, whereas sheep and goats are generally models for reproducing the disease in preclinical experimental studies

Research journal of pharmaceutical, biological and chemical sciences

The ongoing pollution of water resources with a variety of lubricating oils, the insufficiently developed methods of purification of natural and waste water poses the problem of finding ways to restore the natural qualities of the environment. The authors see a solution to this problem in the wide use of activity of individual associations of oil-oxidizing microorganisms (OOM) in combination with the plant-derived sorbents (buckwheat, oat, wheat and barley husk), which allows deep controlled oxidation of these contaminants down to CO2 and H2O. It was found that the multi-species OOM communities take more active part in biodegradation of mineral, semi-synthetic and synthetic oils, than those with the limited species composition. The growth, development and activity in the oil biodegradation is determined by the nature of the contamination. The population growth maxima lie between 5 and 14 days, and decrease to 2 to 6 hours under the influence of sorbents

Intensification of the biotreatment processes of natural and waste water, contaminated with spent lubricating oils with sorbents of various nature

Biodegradation of spent lubricating oils (mineral, semisynthetic, synthetic) was investigated in natural and waste water of consortia of hydrocarbon-oxidizing microorganisms (HOM) under the influence of sorbents of various nature. It was found that the effectiveness of the latter's action on the biotreatment processes of water from oils is determined by the size of particles, concentration, formation of active contact surface of phases by them and their participation in neutralization of contaminants. In direct use of sorbents without HOM, are purified 7 to 19.6 % oils. This is achieved within 10-14 days of contact. The joint use with HOM of inert materials (river sand, coal powder, polyethylene chips, etc.), in the same contact times, water is neutralized from lubricating oils from 44.9 to 63.7 % observed under the influence of HOM only. At the same time, with buckwheat, oats, wheat and barley husks with the same level of pollution, the purification of water from lubricating oils reaches 93.7 %. It is related to the simultaneous participation of these sorbents in the formation of a highly active surface for immobilizing contaminants and microflora, to provide stimulating activity of the latter by involving products associated with these materials in oxidative processes.1-

Biodestruction of lubricated motor oils in sewage water with the use of pilot biological installation

526-532This article presents the results of an experiment on the choice of technology for purification and final purification of wastewater of small sewage enterprises and large-scale productions from waste lubricating oils of mineral origin with a wide change in the pH of the medium, providing dissolved oxygen and biogenic elements. It is proved that a high degree of biodegradation of oil-contaminated effluents is achieved by the wide use of the newly created consortium of hydrocarbon-oxidizing microorganisms (HOM), which includes nine strains belonging to the genera: Alcaligenes(1 species), Bacillus (1 species), Brevibacterium (2 species), Clostridium (1 species), Flavobacterium (1 species), Micrococcus (1 species) and Pseudomonas (2 species). The association of this microorganismsis able to oxidize various fractions of oil ranging from n-alkalis, to asphaltenes, including mineral, semi-synthetic and synthetic oils of various origin. It has been established that the load change from 25 to 100 mg/dm3(COD from 450 to 650 mg/dm3) when using lubricating oil in stock ROB almost does not affect the degree of biodegradation of the original pollution. During the contacting of the ON-NOVO consortium with the oil-containing waste liquid in the bioreactor and further in the secondary settling tank, with a total duration of 1.5 to 4.5 hours, the degree of effluent purification reaches up to 77.4%. With the addition of a normalized amount of nutrients in the amount of 20 mg/dm3 (optimal zone), the degree of neutralization of waste water exceeds 86%. In terms of residual content of oxidized mineral oil, the cleaning intensity exceeds 94%

Process module of oil-containing wastewater treatment of local sewage system and its controlling mathematical model

At contemporary level of development of oil-extracting, oil-processing and oil-chemical industry, with development of car-services' market and growth of number of motor transport establishments, fuel-fiiling stations, car washes and other similar objects, the issue of purification of such enterprises' waste waters becomes more and more critical; this stipulates the necessity of application of technologies, neutralizing this impact. Application of pure bio-technologies with installation of selected micro-organisms providing high indexes of water purification in manageable mode is the most perspective in the field of oil-contaminated industrial waste waters purification. The article "Bio-technical module of hydrocarbon containing waste waters of enterprises of individual sewage system" contains the data on optimization of oil and oil products bio-degradation of OJSC "Kazanorgsyntez" with application of jet-settler (JS), specially created for this purpose, and bringing of all bio-technol

Experimental study on thermal performance in three-flow cooling tower

Biofouling can significantly deteriorate the efficiency of cooling towers. A cooling tower with a three-flow cooling circuit has been developed. The fill pack consists of inclined-corrugated contact elements with perforation. One of the advantages of the proposed cooling tower is the possibility of uniform distribution of liquid over the cross-sectional area. The paper represents the results of an experimental study of the thermal performance of the three-flow cooling tower. It was found that when the wetting rate of the liquid increases, the thermal performance of the cooling tower can reach 47.8%. The developed three-flow cooling tower allows excluding the propagation of microorganisms and reducing the biofouling of industrial water systems along with the high performance for circulating water