Seasonal Variations and Identification of Pathogenic Pollution Indicators from Royapuram Backwaters

Sewage and industrial effluents from Ennore, Chennai harbour and surrounding area have a greater impact on the Royapuram back water affecting the aquatic animals and in tum the human population. These waste carry enormous number of microbial pathogens and other heavy metals resulting in greater economic loss. The current study is aimed at analysing the total viable count of bacteria and pathogenic bacterial species in the water and sediment samples taken from different places of Royapuram fishing area, from where many kind of fishes and molluscans are taken for human consumption .The samples werc collected in pre monsoon, monsoon and post monsoon seasons. Water analysis was done by multiple tube tests to assess the MPN values. The bacteria were isolated using Zobell's agar medium, selective and non selective medias and identified using biochemical test. The organisms were identified to genus, level according to Bergey's manual. Results of field Investigations showed a definite association between the levels pollution and microbial population. Results showed a higher distribution of pathogenic and non pathogenic bacteria in the sediment than in the water sample both in monsoon and post monsoon seasons. Analysis showed the prevalence of human pathogens and fecal indicator organisms like Escherichia coli, Salmonella sp.,Vibrio sp., Shigella sp. in water and in the sediment samples

Identification of Gut Microflora from Green Mussel (Perna viridis)

The main source of Bacterial contamination of Estuary waters are domestic waste water and sewage discharge. In this present investigation, Green Mussels were collected from three different stations of Chennai Coast - Ennore, Royapuram and Muttukadu. The mussels were degutted and bacteria were isolated using selective and non-selective agar medium and further confirmed by biochemical tests. The organisms were ident illed to generic or group level according to Bergey's manual of systematic Bacteriology. Different group of fecal coli forms and Vibrio spp. have been isolated. Escherichia coli, Vibrio alginolyticus, V. parahaemolyticus and V. harveyi were the dominant species. Vibrio parahaemolyticus, a facultative pathogen widely complicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood was present in the gut of Green Mussels

A STUDY ON THE PRESENCE OF FECAL POLLUTION INDICATOR BACTERIA IN MUTTUKADU BACK WATERS, EAST COAST OF TAMIL NADU

ABSTRACT Coliform bacteria are a collection of relatively harmless microorganisms that live in large numbers in the intestines of man, warm and cold-blooded animals. A specific subgroup of this collection is the fecal coliform bacteria, the most common member being Escherichia coli. The presence of fecal coliform bacteria in aquatic environments indicates that the water has been contaminated with the fecal material of man or other animals. The presence of fecal contamination is an indicator that a potential health risk exists for individuals exposed to this water. The current study is aimed at analyzing the presence of fecal coliform in Muttukadu back waters, where different kinds of fishes, prawns and shell fishes are harvested for human consumption. The water samples were collected in pre-monsoon, monsoon and post-monsoon seasons. Water analysis to find the presence of fecal indicator was done by multiple tube tests to assess the MPN values. The presence of Escherichia coli is further confirmed by membrane filtration test and biochemical tests. Analysis showed higher number of fecal indicator in monsoon, followed by post monsoon seasons. The number was comparatively less in pre monsoon seaso