Pulse electric field effect on cervical cancer cell: a plant based anti-cancer study

Cancer is listed in top ten global causes of death in 2016. The percentage of cancer incidence and mortality are steadily rising over the year. This necessitates alternate anti-cancer treatment as the conventional treatment have resulted in high number of long- term survivors who are left to deal with the aftereffect of their therapy. Drug delivery is a challenging aspect in medical community as the plasma membrane is non-permeable to external molecules. From this context, a study was proposed to use of natural plant extract Artocarpus altilis which will be uploaded into cervical cancer cell with the aid of electrical pulses or electroporation technique to investigate on the anti-cancer mechanism. Electroporation parameters utilized in this study ranging from 200V/cm, 400V/cm, 600V/cm, 800V/cm, 1000V/cm of pulse amplitude, while pulse duration ranging from 100μs, 500μs, 1ms, 5ms and 10ms with single pulse. Three parts of artocarpus altilis tested in this research study which is the peel, pulp and whole fruit. The concentration of all three parts which were tested on cervical cancer cell ranged from 10μg/ml, 20μg/ml, 30μg/ml, 40 μg/ml, and 50μg/ml with methanol as the solvent. It is found that pulse electric field increased the cell spreading up to 129.8% with 600V/cm of pulse amplitude, which stimulates to the highest cell length percentage. Apart from that, cell viability found to be decrease with an increase in pulse amplitude and pulse duration. 600V/cm of pulse amplitude and 10ms of pulse duration stimulates highest cell viability with 53.3% as compared to control group (without treatment). Cell viability of cervical cancer cell measured when cell subjected to electrical field alone, extract alone and both electrical field and extract and the result of the study revealed that lowest cell viability stimulated by the combination of electrical pulse and extract up to 28% of cell viability. Thus the outcome of this research revealed new targets and electrochemical pathways for cervical cancer treatment while with the discovery health of patients will be maintained and there will be an improvement in general life expectancy

Artocarpus altilis extract effect on cervical cancer cells

This paper elucidate on the effects of Artocarpus Altilis Pulp part on cervix HeLa cancer cell. IC50 values of pulp extract were determined on HeLa cell with different concentration (12.5µg/ml, 25µg/ml, 50µg/ml, 100µg/ml). Cell viability and cell growth were observed up to 72 hours with comparative to control cells. The results obtained in this research quantitatively revealed the dependence of cell proliferation on extract concentration. Control, 12.5µg/ml, 25µg/ml, 50µg/ml, 100µg/ml of concentration showed 100%, 90%, 80%, 50%, 44% cell viability after 72 hours in culture respectively. This study result demonstrates that Artocarpus Altilis has the ability to inhibit cervical cancer cell proliferation

Influence of artocarpus altilis fruit extract on cancer cell

Conventional chemopreventive agents causes adverse side effects in cancer patients. Thus, this study focuses on the effects of natural plant extract against cancer cell as a way to reduce or detrimental effects of orthodox drugs. The present study emphasizes on anti-cancer potentiality of Artocarpus altilis fruit extract against cervical cancer cell. Cervical cancer cell treated with methanol extract of artocarpus altilis fruit with the concentration varied from 10μg/ml, 20μg/ml, 30μg/ml, 40μg/ml and 50μg/ml. Treated and untreated (cells without treatment) cell proliferation and half maximal inhibitory concentration IC50 measured after 72 hours of incubation. Cells without treatment, 10μg/ml, 20μg/ml, 30μg/ml, 40μg/ml and 50 μg/ml of extract concentration showed 1.3, 0.98, 0.72, 0.65, 0.51, and 0.3 of cell proliferation factor accordingly with 40μg/ml of IC50 value. The result shows that artocarpus altilis is capable in opposing cervical cancer cell proliferation or growth as the proliferation factor decreases with the increasing dose of extract concentration which can be attributed to the presence of bioactive compound in Artocarpus altilis

Wireless Arduino Controlled Fire Fighter Robot Designed for Residential Area

Fire accidents have become a common problem in human life and may lead to permanent injury or death. The fire fighter robot is developed with the aim to save lives of fire fighters who risk their lives, entering dangerous environments. This paper presents the development of the fire fighter robot, which is suitable to extinguish fire in closed areas and residential areas. The robot’s movement can be manually controlled using Flysky GT2 Controller from outside of the affected area. The robot is in the form of a robust and compact vehicle which allows it to move front, back, left and right in small areas with a steady speed. The robot has a 12V water pressure pump capable of shooting water up to 3 to 5 litres per minute which allows it to control large amounts of fire. The robot also provides live streaming and GPS location tracking services during the movement of the robot. This live recording can be viewed in PC or in the user's mobile phone