ANTIDIABETIC ACTIVITY ON METHANOLIC EXTRACTS OF SCHREBERA SWIETENOIDES ROXB. ROOTS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Objective: The aim of study was to evaluate acute toxicity and antidiabetic activity of methanolic extract of Schrebera swietenoides roots in streptozotocin induced diabetic rats.Methods: Acute toxicity of the methanolic extracts of Schrebera swietenoides roots was studied in Wistar albino rats at a dose level upto 2 g/kg b.w. The animals were also closely examined for signs of intoxication, lethargy, behavioral modification and morbidity. The plant was evaluated for antidiabetic activity against Streptozotocininduced diabetic model in different concentrations of 100,200 and 400 mg/kg body weight. The mean and percent blood glucose reduction levels were calculated and comparable with the standard drug, glibenclamide (0.45 mg/kg b. w).Results: The extracts did not produce any toxic signs during the observation period for 24 h. The oral administration of 400 mg/kg b. w of methanolic extract of Schrebera swietenoides roots showed significant (P<0.05) decrease in blood glucose levels up to 18th h, more significant (P<0.01) decrease in blood glucose levels at 4th and 12th h and highly significant (P<0.001) decrease in blood glucose levels at 8th h respectively.Conclusions: From the studies it is concluded that, the methanolic extract of Schrebera swietenoides roots is suitable to prepare ayurvedic formulations for the treatment of diabetes.Â

Development and validation of a new RP-HPLC method for the determination of process related impurities in pioglitazone hydrochloride

A reversed phase high performance liquid chromatographic method for the determination of process related impurities in pioglitazone hydrochloride was developed and validated. High-quality separation was achieved on a Luna C18 column (150 mm × 4.6 mm, 3 µm) using gradient elution at a flow rate of 1 mL/min and a column temperature of 45 °C. UV detection was performed at 254 nm. The validation was performed according to the International Conference on Harmonization (ICH) guidelines. In addition, the solution stability and method robustness were examined. The method gives satisfactory separation of impurities of pioglitazone hydrochloride and so it is suitable for quantification of the process related impurities as well as for the assay of the active compound

DEVELOPMENT AND VALIDATION OF RP-HPLC AND UV SPECTROPHOTOMETRIC METHODS FOR THE QUANTIFICATION OF CAPECITABINE

Objective: The objective of the present study was to develop and validate a new liquid chromatographic technique and four new spectrophotometric methods for the quantitative estimation of Capecitabine.Methods: In the first method, the chromatographic technique was carried out in isocratic technique on Shimadzu Model CBM-20A/20 Alite HPLC system, equipped with SPD M20A prominence PDA detector with Zorbax C18 (150 mm × 4.6 mm i. d, 5 µm particle size) column. The method was optimized with a mobile phase consisting of 0.1 % Acetic acid and Acetonitrile (35:65, v/v) with flow rate 0.5 ml/min. In second, third, fourth and fifth methods, spectrophotometric techniques were applied. The absorption maximum (λmax) was observed at 305 nm, 305 nm, 303 nm and 297 nm for method B (developed in 0.1 N hydrochloric acid), C (developed in sodium acetate buffer pH 4.0), D (developed in phosphate buffer pH 7.0) and E (developed in borate buffer pH 9.0) respectively. Different validation parameters such as linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ), robustness were also determined.Results: The linearity of the calibration curves for the analyte in the desired concentration range is good for both the HPLC (R2 = 0.9994) and UV methods. The LOD and LOQ were found to be 0.02354 μg/ml and 0.07162 μg/ml respectively. The % RSD values for the validation parameters (precision and accuracy) were less than 2.0%.Conclusion: The proposed chromatographic and spectrophotometric methods were validated and can be applied for the determination of Capecitabine in pharmaceutical formulations.Keywords: Capecitabine, UV spectrophotometric, ­­Forced degradation, RP-HPLC, Method validatio

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF IMATINIB MESYLATE AND ITS DIMER IMPURITY IN PHARMACEUTICAL FORMULATION BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

 Objective: The present study is to develop a simple, specific, and validated reverse-phase high-performance liquid chromatography (HPLC) method for the determination of imatinib mesylate and its dimer impurity in pharmaceutical dosage form.Methods: A HPLC instrument incorporated with column HiQ Sil C18 (250 mm × 4.6 mm, 5 μm), mobile phase as methanol and acetate buffer pH 3.5 in the ratio of 80:20 v/v was used for the determination of the imatinib mesylate and its dimer impurity. The detection wavelength was set at 273 nm. The flow rate of the mobile phase was 1.0 mL/min.Results: The retention time for imatinib mesylate was 8.060, and for dimer impurity, it was 11.398. The calibration plot was linear (R2=0.9971) and the % mean recoveries for imatinib mesylate were in the range of 99.83–101.57, and for dimer impurity, it was in the range of 98.16–99.18. The limit of detection concentration was found to be 0.570 μg/ml for imatinib mesylate and 0.033 μg/ml dimer impurity and limit of quantification concentration was 1.728 μg/ml for imatinib mesylate and 0.099 μg/ml dimer impurity.Conclusion: The projected method was validated and successfully functional for the estimation of imatinib mesylate and dimer impurity in formulations. It can be adopted apparently for routine quality control and research tests

EVALUATION OF ANTI-PYRETIC AND WOUNDHEALING ACTIVITIES OF EVOLVULUS NUMMULARIUS LEAVES IN ALBINO WISTAR RATS

Objective: The present study was to evaluate the anti-pyretic and wound healing activity of hydro alcoholic extracts of Evolvulus nummularius leaves in albino wistar rats. Methods: Anti-pyretic potential was determined by yeast-induced pyrexia and for testing the wound healing property, excision method used in wistar albino rats Results: The present result shows that the hydroalcoholc extract of leaf possesses significant anti-pyretic effect in yeast-provoked elevation of body temperature and The hydro alcoholic extract and leaf juices showed a significant wound healing activity which was well comparable with the standard drug used. Conclusions: The most significant inhibition of wound healing and also the significant reduction in hyperpyrexia in rats when treated with standard drugs as well as different extracts

Evaluaton of hypoglycaemic and wound healing activities of Tiliacora acuminata

Natural products particularly plant origin have played a vital role in prevention and mitigation of human diseases. The present study was carried to evaluate the antihyperglycaemic and wound healing activity of the leaf Extracts of Tiliacora acuminate (Lam) on rats, the aqueous leaf extract was found to produce significant reduction of blood glucose concentration between 2-4 h of administration in alloxan induced hyperglycaemic rats at tested dose levels. However, in normoglycaemic animals, the extract at 400mg/kg produced significant reduction of blood glucose between 2-4 h of administration. In the wound healing studies, the leaf juice was found to be more active than the extract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF IMATINIB MESYLATE AND ITS DIMER IMPURITY IN PHARMACEUTICAL FORMULATION BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

 Objective: The present study is to develop a simple, specific, and validated reverse-phase high-performance liquid chromatography (HPLC) method for the determination of imatinib mesylate and its dimer impurity in pharmaceutical dosage form.Methods: A HPLC instrument incorporated with column HiQ Sil C18 (250 mm × 4.6 mm, 5 μm), mobile phase as methanol and acetate buffer pH 3.5 in the ratio of 80:20 v/v was used for the determination of the imatinib mesylate and its dimer impurity. The detection wavelength was set at 273 nm. The flow rate of the mobile phase was 1.0 mL/min.Results: The retention time for imatinib mesylate was 8.060, and for dimer impurity, it was 11.398. The calibration plot was linear (R2=0.9971) and the % mean recoveries for imatinib mesylate were in the range of 99.83–101.57, and for dimer impurity, it was in the range of 98.16–99.18. The limit of detection concentration was found to be 0.570 μg/ml for imatinib mesylate and 0.033 μg/ml dimer impurity and limit of quantification concentration was 1.728 μg/ml for imatinib mesylate and 0.099 μg/ml dimer impurity.Conclusion: The projected method was validated and successfully functional for the estimation of imatinib mesylate and dimer impurity in formulations. It can be adopted apparently for routine quality control and research tests

Hepatoprotective activity of Macrothelypteris torresiana (Gaudich.) aerial parts against CCl4-induced hepatotoxicity in rodents and analysis of polyphenolic compounds by HPTLC

Macrothelypteris torresiana is a fern species belonging to family Thelypteridaceae. The present study was conducted to evaluate hepatoprotective potential of ethanol extract from M. torresiana aerial parts (EEMTAP) and detect the polyphenolic compounds present in the extract using high performance thin layer chromatography (HPTLC). Hepatoprotective potential of EEMTAP were tested at doses of 300 and 600 mg/kg, per os (p.o.), on Wistar albino rats. The extract and silymarin treated animal groups showed significant decrease in activities of different biochemical parameters like serum glutamic oxaloacetic transaminase (SGOT), serum glutamate-pyruvate transaminase (SGPT), alkaline phosphatase (ALP), which were elevated by carbon tetrachloride (CCl4) intoxication. The levels of total bilirubin and total protein alongwith the liver weight were also restored to normalcy by EEMTAP and silymarin treatment. After CCl4 administration the level of hepatic antioxidant enzymes such as Glutathione (GSH) and Catalase (CAT) were decreased whereas the level of hepatic lipid peroxidation (LPO) was elevated. The level of these hepatic antioxidant enzymes were also brought to normalcy by EEMTAP and silymarin treatment. Histological studies supported the biochemical findings and treatment with EEMTAP at doses 300 and 600 mg/kg, p.o. was found to be effective in restoring CCl4-induced hepatotoxicity in rats. A simple HPTLC analysis was conducted for the detection of polyphenolic compounds in EEMTAP, and the result revealed the presence of caffeic acid as phenolic acid and quercetin as flavonoid. The proposed HPTLC method is simple, concise and provides a good resolution of caffeic acid and quercetin from other constituents present in EEMTAP

C-Prenylflavonoids from Derris heyneana

2′,4′-Dihydroxy-3′-(3-methylbut-2-enyl)-4-methoxychalcone (1) and three known C-prenylflavonoids, namely, lupinifolin (flemichin B), hiravanone, and derrubone were isolated from the leaves of Derris heyneana (Febaceae). The structures were established by 2D NMR analysis

Flavonoids of Tephrosia Procumbens

Objective : The genus Tephrosia is a rich source for unusual flavonoids. The present work is aimed to isolate new molecules of biological interest. Materials and methods : The root powder of T. procumbens was extracted with chloroform and concentrated. The residue was subjected to column chromatography and fractional crystallization by organic solvents and their mixtures of increasing polarity which afforded crystalline compounds and were identified by spectroscopic methods. Results and conclusions : Among the isolates, two candidates calopogonium isoflavone B and 2\u27, 7\u27 8-trimethoxy-4\u27 , 5\u27 -methylenedioxy isoflavone are reported for the first time from this species