From the Ground Up: Strengthening Health Systems at District Level (Introduction)

This supplement introduces the African Health Initiative (AHI), a research program comprised of five unique district health system-strengthening activities in Ghana, Mozambique, Rwanda, Tanzania, and Zambia that beganin 2009. This supplement should be of interest to all engaged in improving delivery of district primary health care -- whether ministries of health, service providers, funders, or those who evaluate complex interventions. The five AHI projects, known as Population Health Implementation and Training (PHIT) Partnerships, are funded by the Doris Duke Charitable Foundation (DDCF) with a common goal: to produce significant, measureable health improvements in a defined geographic area over a five to seven year grant period.With the partnerships in their fourth year of funding, it is now possible to capture lessons learned in project design and implementation. Evaluation of the African Health Initiative's impact on population health, including mortality, however, mustawait the conclusion of the grant period

The Doris Duke Clinical Scientist Development Award: A Seven-Year Retrospective and Summary

During an interval of 15 years, the Lucille P. Markey Charitable Trust spent over $500 million on four programs in the basic biomedical sciences that support the education and research of graduate students, postdoctoral fellows, junior faculty, and senior researchers. The Markey Trust asked the NRC to evaluate these programs with two questions in mind: "Were these funds well spent?" and "What can others in the biomedical and philanthropic communities learn from the programs of the Markey Trust, both as an approach to funding biomedical research and as a model of philanthropy?" One of five resulting reports, this volume contains the proceedings of a workshop held in June 2005 to investigate methods used to evaluate funding of the biomedical scientists by philanthropic and public funders. In addition to the Markey Trust, representatives from the Howard Hughes Medical Institute, the American Heart Association, the Doris Duke Charitable Foundation, the National Institutes of Health, and six other funders of biomedical scientists presented information on evaluation methodologies and outcome

Medical Reserach Program Bulletin

This bulletin summarizes many of the past year’s activities and highlights some of our grantees. Our focus remains to help strengthen and support clinical research in order to speed the translation of basic research findings into new cures, therapies and preventions for human diseases. We are pleased to have been able to sponsor three new grant competitions, which in 2005 awarded grants ranging in size from $170,000 to$2.25 million

The formyl peptide chemoattractant receptor is encoded by a 2 kilobase messenger RNA Expression in Xenopus oocytes

AbstractActivation of the formyl peptide chemoattractant receptor (FPCR) of phagocytic cells mobilizes intracellular calcium stores and affects the plasma membrane potential. Affinity crosslinking of FPCR has demonstrated a 60–80 kDa glycoprotein, with core peptide of 32 kDa. It is not known whether functional FPCR is this single peptide or requires multiple subunits. We used Xenopus oocyte expression system to determine the size of mRNA required for synthesis of functional FPCR. Injection of oocytes with poly(A)+ RNA from HL60 cells differentiated to the granulocyte phenotype resulted in acquisition of formyl peptide-specific responses (inward transmembrane current with a reversal potential consistent with a chloride conductance, and calcium efflux). FPCR activity expressed in oocytes had a ligand concentration dependence, ligand structure dependence and pertussis toxin sensitivity similar to those reported in phagocytic cells. When RNA was size fractionated, a single peak of FPCR activity at 2 kilobases was observed after injection of mRNA into oocytes. Our data strongly suggest that FPCR is composed of a single-sized polypeptide