Dust in dwarf galaxies: The case of NGC 4214

We have carried out a detailed modelling of the dust heating and emission in the nearby, starbursting dwarf galaxy NGC 4214. Due to its proximity and the great wealth of data from the UV to the millimeter range (from GALEX, HST, {\it Spitzer}, Herschel, Planck and IRAM) it is possible to separately model the emission from HII regions and their associated photodissociation regions (PDRs) and the emission from diffuse dust. Furthermore, most model parameters can be directly determined from the data leaving very few free parameters. We can fit both the emission from HII+PDR regions and the diffuse emission in NGC 4214 with these models with "normal" dust properties and realistic parameters.Comment: 4pages, 3 figures. To appear in 'The Spectral Energy Distribution of Galaxies' Proceedings IAU Symposium No 284, 201

Ten simple rules for aspiring graduate students.

Several supervillains have higher degrees—why don’t you? There can be a variety of reasons for wanting to go to grad school and for applying to a particular school and program. But often, one can only tell apart good and bad reasons from hindsight. Failing at something is perhaps the best way to know what can go wrong and what advice would have been useful when considering graduate school applications.We should know: one of us started graduate school 4 separate times, and another learned what a PhD was only after having started one; lost 2 supervisors before even starting to write her thesis, and yet another accepted a PhD offer from the lab where she was working, without considering any alternatives; finally, one of us had applied to graduate schools for 5 years (with 19 rejections) before finally landing a PhD offer from their dream school. We hope that our hard-earned lessons will help you to avoid some of the pitfalls that we ourselves fell prey to. In this article, we address how to choose a graduate program, how to apply strategically, and some of the key challenges that may arise along the way toward graduate school. Conveniently, our advice can be summarized as 10 simple rules . . . so here they are.The authors acknowledge the support of the Gates Cambridge Trust [AIL], Cancer Research UK [RRG], Alzheimer’s Society [CCN], Merck [CCN], and the Isaac Newton Trust [EG]

Analysis of Serum Th2 Cytokines in Infants with Non-IgE Mediated Food Allergy Compared to Healthy Infants

Background: The aim of this study is to assess the serum values of IL-4, IL-5, IL-10, and IL-13 in a group of infants with non-IgE mediated food allergies treated with a hydrolyzed formula and compare them with a group of healthy peers. Methods: A total of 53 infants aged 1 to 4 months, of which 34 with non-IgE mediated food allergies and 19 healthy infants were enrolled in this study. Infants were eligible if they had gastrointestinal symptoms of food allergy and needed to switch from their initial formula to hydrolyzed formulas with an improvement of symptoms. Controls were fed with either breastmilk or standard formula. Blood samples were taken within one week of a special diet for cases. Interleukinsin in peripheral blood was detected and analyzed using the real-time PCR MAMA method. Fecal calprotectin was evaluated using a quantitative assay. Results: Values of IL-4 and IL-13 were significantly higher in the non-IgE food allergy group compared to the control group (p < 0.05), while IL-5 and IL-10 were significantly lower than the control group (p < 0.05). Fecal calprotectin in the non-IgE food allergy group was significantly higher compared to the control group (p < 0.05). Conclusion: This study provides a theoretical basis that Th2 cytokine expression in infants with a non-IgE mediated food allergy is significantly different than in healthy infants; this finding supports the use of early dietetic treatment with hydrolyzed formulas

Improved Molecular Diagnosis of McCune-Albright Syndrome and Bone Fibrous Dysplasia by Digital PCR

McCune-Albright syndrome (MAS) is a rare congenital disorder characterized by the association of endocrine and nonendocrine anomalies caused by somatic activating variants of GNAS. The mosaic state of variants makes the clinical presentation extremely heterogeneous depending on involved tissues. Biological samples bearing a low level of mosaicism frequently lead to false-negative results with an underestimation of causative molecular alterations, and the analysis of biopsies is often needed to obtain a molecular diagnosis. To date, no reliable analytical method for the noninvasive testing of blood is available. This study was aimed at validating a novel and highly sensitive technique, the digital PCR (dPCR), to increase the detection rate of GNAS alterations in patients with a clinical suspicion of MAS and, in particular, in blood. We screened different tissues (blood, bone, cutis, ovary, and ovarian cyst) collected from 54 MAS patients by different technical approaches. Considering blood, Sanger was unable to detect mutations, the allele-specific PCR and the co-amplification at lower denaturation temperature had a 9.1% and 18.1% detection rate, respectively, whereas the dPCR reached a 37.8% detection rate. In conclusion, the dPCR resulted in a cost-effective, reliable, and rapid method allowing the selective amplification of low-frequency variants and able to improve GNAS mutant allele detection, especially in the blood