Are aerobic fitness and repeated sprint ability linked to fatigue in professional soccer match-play? A pilot study

This investigation examined the association between aerobic fitness and repeated sprint ability and match-related fatigue in 9 professional outfield soccer players. Aerobic fitness using maximal aerobic speed (MAS) was determined via a continuous progressive incremental running test conducted on a motorised treadmill. A repeated sprint ability test (6 successive 6 s sprints separated by 20 s passive recovery) was performed on a non-motorised treadmill to determine mean and best sprint times and a percentage decrement score (%PD). A total of 114 observations of physical performance derived using computerised time motion analyses were collected from 33 matches. Correlations between fitness test and match-play measures were examined for 1) accumulated fatigue: percentage difference between halves for total distance covered per minute, distance run at high-intensities (HIR, actions for 1s duration, >19.1 km/h) per minute, mean recovery time between high-intensity runs, and percentage difference between the distance covered in HIR in the first 5- and 15-minute periods versus the final 5- and 15-minute periods respectively in normal time; and for 2) transient fatigue: percentage difference between the distance covered in HIR in a peak 5-minute period and the subsequent 5-minute period and for the latter compared to the mean for all other 5-minute periods. No significant relationships were observed between MAS and fatigue scores (magnitude of associations: trivial to large). For mean and best sprint times and %PD, the only reported significant correlation (r=0.77, magnitude of association: very large, p<0.05) was between %PD and the % difference across halves for mean recovery time between high-intensity runs (magnitude of other associations: small to large). Criterion measures from tests of aerobic fitness and repeated sprint ability might not accurately depict a player’s capacity to resist fatigue during professional soccer competition

Squad management, injury and match performance in a professional soccer team over a Championship-winning season

Squad management, injury and physical, tactical and technical match performance were investigated in a professional soccer team across five consecutive league seasons (2008–2013, 190 league games) with specific focus on a championship-winning season (2010/11). For each player, match participation and time-loss injuries were recorded, the latter prospectively diagnosed by the team's physician. Defending and attacking tactical and technical performance indicators investigated included ball possession and possession in opponents' half, passes, forward passes, completed passes and forward passes, crosses and completed crosses, goal attempts and goal attempts on target, successful final third entries, free-kicks and 50/50 duels won/lost. Physical performance measures included total distance and distance covered at high-speeds (≥19.1 km/h). Results showed that during the 2010/11 season, squad utilisation was lowest potentially owing to the observed lower match injury occurrence and working days lost to injury thereby increasing player availability. In 2010/11, the team won both its highest number of points and conceded its lowest number of goals especially over the second half of this season. The team also won its highest number of games directly via a goal from a substitute and scored and conceded a goal first on the highest and lowest number of occasions, respectively. While multivariate analysis of variance (MANOVA) detected a significant difference in some attacking and defensive performance indicators across the five seasons, these were generally not distinguishing factors in 2010/11. Similarly, univariate ANOVAs showed a significant difference in running distances covered across seasons, but the trend was for less activity in 2010/11

Penicillin kills chlamydia following the fusion of bacteria with Lysosomes and prevents genital inflammatory lesions in C. muridarum-infected mice

The obligate intracellular bacterium Chlamydia exists as two distinct forms. Elementary bodies (EBs) are infectious and extra-cellular, whereas reticulate bodies (RBs) replicate within a specialized intracellular compartment termed an ‘inclusion’. Alternative persistent intra-cellular forms can be induced in culture by diverse stimuli such as IFNγ or adenosine/EHNA. They do not grow or divide but revive upon withdrawal of the stimulus and are implicated in several widespread human diseases through ill-defined in vivo mechanisms. β-lactam antibiotics have also been claimed to induce persistence in vitro. The present report shows that upon penicillin G (pG) treatment, inclusions grow as fast as those in infected control cells. After removal of pG, Chlamydia do not revert to RBs. These effects are independent of host cell type, serovar, biovar and species of Chlamydia. Time-course experiments demonstrated that only RBs were susceptible to pG. pG-treated bacteria lost their control over host cell apoptotic pathways and no longer expressed pre-16S rRNA, in contrast to persistent bacteria induced with adenosine/EHNA. Confocal and live-video microscopy showed that bacteria within the inclusion fused with lysosomal compartments in pG-treated cells. That leads to recruitment of cathepsin D as early as 3 h post pG treatment, an event preceding bacterial death by several hours. These data demonstrate that pG treatment of cultured cells infected with Chlamydia results in the degradation of the bacteria. In addition we show that pG is significantly more efficient than doxycycline at preventing genital inflammatory lesions in C. muridarum-C57Bl/6 infected mice. These in vivo results support the physiological relevance of our findings and their potential therapeutic applications

Electron-nuclei spin dynamics in II-VI semiconductor quantum dots

We report on the dynamics of optically induced nuclear spin polarization in individual CdTe/ZnTe quantum dots loaded with one electron by modulation doping. The fine structure of the hot trion (charged exciton $X^-$ with an electron in the $P$-shell) is identified in photoluminescence excitation spectra. A negative polarisation rate of the photoluminescence, optical pumping of the resident electron and the built-up of dynamic nuclear spin polarisation (DNSP) are observed in time-resolved optical pumping experiments when the quantum dot is excited at higher energy than the hot trion triplet state. The time and magnetic field dependence of the polarisation rate of the $X^-$ emission allows to probe the dynamics of formation of the DNSP in the optical pumping regime. We demonstrate using time-resolved measurements that the creation of a DNSP at B=0T efficiently prevents longitudinal spin relaxation of the electron caused by fluctuations of the nuclear spin bath. The DNSP is built in the microsecond range at high excitation intensity. A relaxation time of the DNSP in about 10 microseconds is observed at $B=0T$ and significantly increases under a magnetic field of a few milli-Tesla. We discuss mechanisms responsible for the fast initialisation and relaxation of the diluted nuclear spins in this system

A Tool for Visual Understanding of Source Code Dependencies

Many program comprehension tools use graphs to visualize and analyze source code. The main issue is that existing approaches create graphs overloaded with too much information. Graphs contain hundreds of nodes and even more edges that cross each other. Understanding these graphs and using them for a given program comprehension task is tedious, and in the worst case developers stop using the tools. In this paper we present DA4Java, a graphbased approach for visualizing and analyzing static dependencies between Java source code entities. The main contribution of DA4Java is a set of features to incrementally compose graphs and remove irrelevant nodes and edges from graphs. This leads to graphs that contain significantly fewer nodes and edges and need less effort to understand

Lichens of six vernal pools in Acadia National Park, ME, USA

Whereas lichen-habitat relations have been well-documented globally, literature on lichens of vernal pools is scant. We surveyed six vernal pools at Acadia National Park on Mount Desert Island, Maine, USA for their lichen diversity. Sixty-seven species were identified, including seven species that are new reports for Acadia National Park: Fuscidea arboricola, Hypogymnia incurvoides, Lepraria finkii, Phaeographis inusta, Ropalospora viridis, Usnea flammea, and Violella fucata. Five species are considered uncommon or only locally common in New England: Everniastrum catawbiense, Hypogymnia krogiae, Pseudevernia cladonia, Usnea flammea, and Usnea merrillii. This work represents the first survey of lichens from vernal pools in Acadia National Park and strongly suggests that previous efforts at documenting species at the Park have underestimated its species diversity. More work should be conducted to determine whether a unique assemblage of lichens occurs in association with this unique habitat type