Development and characterization of microsatellite loci for Ocotea species (Lauraceae) threatened with extinction

The Atlantic rainforest species Ocotea catharinensis, Ocotea odorifera, and Ocotea porosa have been extensively harvested in the past for timber and oil extraction and are currently listed as threatened due to overexploitation. To investigate the genetic diversity and population structure of these species, we developed 8 polymorphic microsatellite markers for O. odorifera from an enriched microsatellite library by using 2 dinucleotide repeats. The microsatellite markers were tested for cross-amplification in O. catharinensis and O. porosa. The average number of alleles per locus was 10.2, considering all loci over 2 populations of O. odorifera. Observed and expected heterozygosities for O. odorifera ranged from 0.39 to 0.93 and 0.41 to 0.92 across populations, respectively. Cross-amplification of all loci was successfully observed in O. catharinensis and O. porosa except 1 locus that was found to lack polymorphism in O. porosa. Combined probabilities of identity in the studied Ocotea species were very low ranging from 1.0 x 10-24 to 7.7 x 10-24. The probability of exclusion over all loci estimated for O. odorifera indicated a 99.9% chance of correctly excluding a random nonparent individual. The microsatellite markers described in this study have high information content and will be useful for further investigations on genetic diversity within these species and for subsequent conservation purposes13351385142COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESsem informaçã

Construção de uma biblioteca de cDNA de nódulos de caupi e isolamento de genes expressos em condições de estresse térmico.

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Identificação de genes do feijoeiro envolvidos na simbiose com o Rhizobium tropici.

Made available in DSpace on 2011-04-09T14:12:19Z (GMT). No. of bitstreams: 1 46IdentificacaodegenesdofeijoeiroenvolvidosnasimbiosecomoRhizobiumtropici.pdf: 75409 bytes, checksum: a4304c15363174440b4da9153f784131 (MD5) Previous issue date: 2011-02-04201

Identificação de genes do feijoeiro envolvidos na simbiose com o Rhizobium tropici.

Made available in DSpace on 2011-04-09T14:12:19Z (GMT). No. of bitstreams: 1 46IdentificacaodegenesdofeijoeiroenvolvidosnasimbiosecomoRhizobiumtropici.pdf: 75409 bytes, checksum: a4304c15363174440b4da9153f784131 (MD5) Previous issue date: 2011-02-04201

Prospecção de genes diferencialmente expressos em nódulos de feijão-caupi submetido a estresse térmico e déficit hídrico.

Made available in DSpace on 2011-04-09T14:12:52Z (GMT). No. of bitstreams: 1 Prospeccaodegenesdiferencialmenteexpressosemnodulosdefeijaocaupisubmetido.pdf: 14846 bytes, checksum: 393799e2d68573cfab1b3540bf088a8f (MD5) Previous issue date: 2011-02-08201

Response of traditional upland rice varieties to inoculation with selected diazotrophic bacteria isolated from rice cropped at the northeast region of Brazil.

The largest numbers of the Brazilian traditional upland rice varieties are found in the Maranhão state, Northeast region of Brazil. However, no information is available on the diazotrophic bacterial population associated as well as the plant growth promoting potential when these traditional genotypes are inoculated with native strains. Here, we evaluated the response of ten traditional rice varieties to inoculation with ten diazotrophic strains, previously isolated from rice soil of this region and screened for their ability to produce indole-3-acetic acid (IAA) in vitro. The procedure for selection of the best diazotrophic strain/rice variety interaction involved three steps: gnotobiotic conditions, soil pot and field experiments. The gnotobiotic experiment showed that the Azospirillum amazonense strain AR3122 increased the biomass of the traditional varieties Cana Roxa and Cana Forte (28 and 48%, respectively) while this effect was less evident for the other combination of strains/rice varieties. The soil pot experiment showed that the combination of Burkholderia vietnamiensis strain AR 1122 and traditional variety Arroz 70 was superior to the other strains/varieties and the treatment fertilized with 100 kgNha−1. The best performance of the Burkholderia vietnamiensis strain AR1122/variety Arroz 70 was confirmed in the field experiment. There was an increase of up 10 and 29% in the grain yield in comparison to both the N fertilization and Herbaspirillum seropedicae ZAE 94 strain treatments, respectively. In contrast, the response of the commercial variety Bonanc¸ a to inoculation with strain AR1122 was much lower, suggesting that a biofertilizer inoculation program for traditional rice varieties should consider the genetic interaction between strain and rice variety. The diazotrophic B. vietmaniensis strain AR1122 was a good biofertilizer candidate for inoculation of traditional rice varieties and therefore should be used for further studies to confirm the strain-genotype effect envisaging a sustainable rice crop system mainly in the Northeast region of Brazil.201