Kinetics of Glycoxidation of Bovine Serum Albumin by Glucose, Fructose and Ribose and Its Prevention by Food Components

The aim of this study was to compare the kinetics of the glycoxidation of bovine serum albumin (BSA) as a model protein by three sugars: glucose, fructose and ribose, using fluorometric measurements of the content of advanced glycation end products (AGEs), protein-bound fructosamine, dityrosine, N'-formylkynurenine, kynurenine, tryptophan, the content of advanced oxidation protein products (AOPP), protein carbonyl groups, as well as thiol groups. Moreover, the levels of glycoalbumin and AGEs were determined by using an enzyme-linked immunosorbent assay. Based on the kinetic results, the optimal incubation time for studies of the modification of the glycoxidation rate by additives was chosen, and the effects of 25 compounds of natural origin on the glycoxidation of BSA induced by various sugars were examined. The same compounds were found to have different effects on glycoxidation induced by various sugars, which suggests caution in extrapolation from experiments based on one sugar to other sugars. From among the compounds tested, the most effective inhibitors of glycoxidation were: polyphenols, pyridoxine and 1-cyano-4- hydroxycinnamic acid.The study has been supported by Grant 2011/01/M/NZ3/02065 from the Polish National Science Center and performed within the COST (European Cooperation in Science and Technology) action CM1001

Kinetics of Glycoxidation of Bovine Serum Albumin by Methylglyoxal and Glyoxal and its Prevention by Various Compounds

The aim of this study was to compare several methods for measurement of bovine serum albumin (BSA) modification by glycoxidation with reactive dicarbonyl compounds (methylglyoxal ‒ MGO and glyoxal ‒ GO), for studies of the kinetics of this process and to compare the effects of 19 selected compounds on BSA glycation by the aldehydes. The results confirm the higher reactivity of MGO with respect to GO and point to the usefulness of AGE, dityrosine and N′-formylkynurenine fluorescence for monitoring glycation and evaluation of protection against glycation. Different extent of protection against glycation induced by MGO and GO was found for many compounds, probably reflecting effects on various stages of the glycation process. Polyphenols (genistein, naringin and ellagic acid) were found to protect against aldehyde-induced glycation; 1-cyano-4-hydroxycinnamic acid was also an effective protector.The study has been supported by Grant 2011/01/M/NZ3/02065 from the Polish National Science Center. We are indebted to J. Skolimowski for the synthesis of nitroxide

Oxidative Modification of Proteins in Pediatric Cystic Fibrosis with Bacterial Infections

Pseudomonas aeruginosa and Staphylococcus aureus cause chronic lung infection in cystic fibrosis (CF) patients, inducing chronic oxidative stress. Several markers of plasma protein oxidative damage and glycoxidation and activities of erythrocyte antioxidant enzymes have been compared in stable CF patients chronically infected with Pseudomonas aeruginosa ( = 12) and Staphylococcus aureus ( = 10) in relation to healthy subjects ( = 11). Concentration of nitric oxide was also measured in the exhaled air from the lower respiratory tract of patients with CF. Elevated glycophore (4.22 ± 0.91 and 4.19 ± 1.04 versus control 3.18 ± 0.53 fluorescence units (FU)/mg protein; < 0.05) and carbonyl group levels (1.9 ± 0.64, 1.87 ± 0.45 versus control 0.94 ± 0.19 nmol/mg protein; < 0.05) as well as increased glutathione S-transferase activity (2.51 ± 0.88 and 2.57 ± 0.79 U/g Hb versus 0.77 ± 0.16 U/g Hb; < 0.05) were noted in Pseudomonas aeruginosa and Staphylococcus aureus infected CF. Kynurenine level (4.91 ± 1.22 versus 3.89 ± 0.54 FU/mg protein; < 0.05) was elevated only in Staphylococcus aureus infected CF. These results confirm oxidative stress in CF and demonstrate the usefulness of the glycophore level and protein carbonyl groups as markers of oxidative modifications of plasma proteins in this diseaseThe study has been supported by the Polish Ministry of Science and Higher Education with an Iuventus Plus Grant IP201104797

The reclamation of post-mining areas of Lubuski Region (Poland) on example of Sieniawa Lignite Mine

Tyt. z nagł.Bibliogr. s. [187].KWB &quot;Sieniawa&quot; jest jedną z najstarszych w Polsce kopalń, która eksploatowała węgiel brunatny metodą podziemną oraz metodą odkrywkową. Ten drugi sposób wydobycia realizowany jest do dzisiaj. Skutki związane z eksploatacją węgla brunatnego najbardziej widoczne są w przeobrażającym się krajobrazie, dlatego bardzo ważnym elementem działań kopalń na rzecz środowiska naturalnego jest systematyczne prowadzenie rekultywacji terenów poprzemysłowych. W artykule autorzy przedstawiają i charakteryzują dotychczasowe doświadczenia i przebieg procesu rekultywacji o kierunku leśnym terenów poeksploatacyjnych powstałych wskutek działalności Kopalni Węgla Brunatnego &quot;Sieniawa&quot;.Lignite Mine &quot;Sieniawa&quot; is one of the oldest mine in Poland, which extracted lignite using both underground and open pit method. The latter process has been carried out until present day. The effects of the lignite mining extraction are most evident in transformation of the landscape, and therefore systematic approach to the rehabilitation of brownfield sites is a very important element of the mine's environmental policy. The article presents the actual state of land reclamation for forestry of the post-mining areas of the lignite opencast mine of KWB &quot;Sieniawa&quot;.Dostępny również w formie drukowanej.SŁOWA KLUCZOWE: węgiel brunatny, rekultywacja, górnictwo odkrywkowe, KWB Sieniawa, lignite. KEYWORDS: Sieniawa Lignite Mine, land reclamation, open pit mining

Praktyka sozotechniczna w działalności górniczej KWB &quot;Sieniawa&quot;

Tyt. z nagłówka.Bibliogr. s. [86].Dostępny również w formie drukowanej.STRESZCZENIE: KWB &quot;Sieniawa&quot; jest jedyną w Polsce kopalnią, która eksploatowała węgiel brunatny metodą podziemną oraz metodą odkrywkową. Ten drugi sposób wydobycia realizowany jest do dzisiaj. Skutki związane z eksploatacją węgla brunatnego najbardziej widoczne są w przeobrażającym się krajobrazie, dlatego bardzo ważnym elementem działań kopalń na rzecz środowiska naturalnego jest systematyczne prowadzenie rekultywacji terenów poprzemysłowych. Dowodem takiego właściwego podejścia do problemów sozologicznych i sozotechniczych w branży górnictwa węgla brunatnego jest kopalnia &quot;Sieniawa&quot;. SŁOWA KLUCZOWE: węgiel brunatny, rekultywacja, sozotechnika, górnictwo odkrywkowe. ABSTRACT: Lignite Mine &quot;Sieniawa&quot; is the only mine in Poland, which extracted lignite using both underground and open pit method. The latter extraction is carried out until present day. The effects of the lignite mining process are most evident in transformation of the landscape, and therefore systematic approach to the rehabilitation of brownfield sites is a very important element of the mine's environmental policy. Lignite Mine &quot;Sieniawa&quot; may serve as an example of such correct approach to the sozological and sozotechnical problems encountered in open pit mining. KEYWORDS: land reclamation, lignite, sozotechnique, open pit mining

Dotychczasowe doświadczenia rekultywacji wyrobisk po eksploatacji złoża węgla brunatnego &quot;Sieniawa&quot;

Tyt. z nagłówka.Bibliografia s. [178].Dostępny również w formie drukowanej.STRESZCZENIE: W artykule autorzy przedstawiają i charakteryzują dotychczasowe doświadczenia i przebieg procesu rekultywacji terenów poeksploatacyjnych powstałych wskutek działalności najmniejszej a zarazem najciekawszej (dzięki swej historii) Kopalni Węgla Brunatnego &quot;Sieniawa&quot;. Prawidłowo przeprowadzona rekultywacja daje szansę na zminimalizowanie negatywnych skutków ingerencji górniczej w środowisko naturalne i powrót do warunków zbliżonych do stanu sprzed eksploatacji. SŁOWA KLUCZOWE: górnictwo odkrywkowe, węgiel brunatny, rekultywacja, tereny górnicze. ABSTRACT: The article presents the actual state of reclamation of the post-mining areas of the lignite opencast mine of KWB &quot;Sieniawa&quot; Sp. z o.o. Development of &quot;Sieniawa&quot; Mine is discussed the issues pertaining to the transformation the land surface resulting from the conducted mining operations, as well as the overall prevailing conditions that are related to land reclamation and development. Data that characterize the reclamation of lands for forestry, agriculture and water management purposes are presented. KEYWORDS: open pit mining, lignite, reclamation, post mining terrains

Monitoring the quality of water from isolated marshes resulting from opencast and underground mining of the Sieniawa lignite deposit

The „Sieniawa” lignite mine is one of the oldest Polish mines extracting lignite by means of both underground and opencast mining. The latter process is carried out until the present day. The transformation of the landscape is the most visible evidence of carrying out mining activities and therefore systematic approach to the reclamation of brownfield sites is a very important element of the mine’s environmental policy. The authors present previous experiences and the course of reclamation of post-mining terrains created during the operation of the “Sieniawa” lignite mine. The greatest emphasis is placed on numerous small anthropogenic reservoirs formed as a result of spontaneous flooding due to underground exploitation (surface subsidence) and filling of open pits with water

Modification of the deoxyribose test to detect strong iron binding

Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe2+ complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe2+ in the Fenton reaction, on the basis of examination of concentration dependence of deoxyribose degradation on Fe2+ concentration, at a constant concentration of a chelating agent

Fenton reaction

Toksyczność żelaza jest uwarunkowana reakcją Fentona czyli tworzeniem rodników hydroksylowych w reakcji z nadtlenkiem wodoru. Artykuł przedstawia skrótowo historię odkrycia Fentona, kontrowersję dotyczącą natury reaktywnego oksydanta tworzonego w reakcji Fentona, reaktywność rodnika hydroksylowego, udział jonów innych metali w reakcji Fentona i wykorzystanie reakcji Fentona do oczyszczania ścieków.The toxicity of iron is mainly due to the Fenton reaction i. e. formation of hydroxyl radicals in a reaction with hydrogen peroxide. The paper presents briefly the history of Fenton discovery, the controversy regarding the nature of the reactive oxidant formed in the Fenton reaction, the reactivity of the hydroxyl radical, participation of other metal ions in the Fenton reaction and the application of the Fenton reaction for wastewater treatment

Oxidative modification of proteins in pediatric cystic fibrosis with bacterial infections,” Oxidative Medicine and Cellular Longevity,

Pseudomonas aeruginosa and Staphylococcus aureus cause chronic lung infection in cystic fibrosis (CF) patients, inducing chronic oxidative stress. Several markers of plasma protein oxidative damage and glycoxidation and activities of erythrocyte antioxidant enzymes have been compared in stable CF patients chronically infected with Pseudomonas aeruginosa ( = 12) and Staphylococcus aureus ( = 10) in relation to healthy subjects ( = 11). Concentration of nitric oxide was also measured in the exhaled air from the lower respiratory tract of patients with CF. Elevated glycophore (4.22 ± 0.91 and 4.19 ± 1.04 versus control 3.18 ± 0.53 fluorescence units (FU)/mg protein; &lt; 0.05) and carbonyl group levels (1.9 ± 0.64, 1.87 ± 0.45 versus control 0.94 ± 0.19 nmol/mg protein; &lt; 0.05) as well as increased glutathione S-transferase activity (2.51 ± 0.88 and 2.57 ± 0.79 U/g Hb versus 0.77 ± 0.16 U/g Hb; &lt; 0.05) were noted in Pseudomonas aeruginosa and Staphylococcus aureus infected CF. Kynurenine level (4.91 ± 1.22 versus 3.89 ± 0.54 FU/mg protein; &lt; 0.05) was elevated only in Staphylococcus aureus infected CF. These results confirm oxidative stress in CF and demonstrate the usefulness of the glycophore level and protein carbonyl groups as markers of oxidative modifications of plasma proteins in this disease